@article{62190,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Lignin, a widely available and renewable organic polymer, has several desirable properties and applications. However, as a by‐product of pulp and paper industry, it is mainly burned for energy. Limited understanding of the complex and heterogeneous structure and a shortage of tailored analysis methods hinder its utilization in higher value applications. This study describes and compares the use of two different static light scattering methods, laser diffraction and small‐angle light scattering (SALS), for studying lignin particle size in suspension. The results from laser diffraction showed that the selected particle concentration and absorption coefficient affect the measured sizes especially for particles &lt;1 µm in diameter. For irregularly shaped particles with broad size distributions, sampling is the most important parameter affecting the results. SALS proved an efficient method for obtaining information on particle aggregation by providing primary particle sizes as well as aggregate sizes. Characterization of samples with spherical particles and narrow size distributions is straightforward with both laser diffraction and SALS, whereas the interpretation of results for more heterogeneous samples is less obvious. Static light scattering methods could make lignin particle size analysis more rapid and automated, thus enhancing lignin valorization, but should be applied carefully to avoid systematic errors.</jats:p>}},
  author       = {{Makkonen, Janita and Ahvenainen, Patrik and Bertella, Stefania and Kellock, Miriam and Saha, Sanjib and Huber, Klaus and Farooq, Muhammad and Österberg, Monika and Penttilä, Paavo}},
  issn         = {{0934-0866}},
  journal      = {{Particle &amp; Particle Systems Characterization}},
  publisher    = {{Wiley}},
  title        = {{{Static Light Scattering for Lignin Particle Size Characterization}}},
  doi          = {{10.1002/ppsc.202500085}},
  year         = {{2025}},
}

@article{62180,
  author       = {{Koch, Leon and Rajput, Satyendra and Richter, Antonio and König, Benedikt and Nayar, Divya and Ebbinghaus, Simon and Huber, Klaus}},
  issn         = {{1520-6106}},
  journal      = {{The Journal of Physical Chemistry B}},
  number       = {{25}},
  pages        = {{6115--6126}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Self-Assembly of Pseudo Isocyanine Chloride in the Presence of Attractive Polyethylene Glycol Crowders}}},
  doi          = {{10.1021/acs.jpcb.4c06843}},
  volume       = {{129}},
  year         = {{2025}},
}

@article{62189,
  author       = {{Kollmann, Fabian and Büngeler, Anne and Splett, Miriam and Strube, Oliver I. and Huber, Klaus}},
  issn         = {{1525-7797}},
  journal      = {{Biomacromolecules}},
  number       = {{5}},
  pages        = {{3104--3112}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Analysis of the Growth Mechanism of Eumelanin Particles by Time-Resolved Static and Dynamic Light Scattering}}},
  doi          = {{10.1021/acs.biomac.5c00158}},
  volume       = {{26}},
  year         = {{2025}},
}

@article{62177,
  author       = {{Huber, Klaus and Martens, C.M. and Tuinier, R.}},
  issn         = {{0021-9797}},
  journal      = {{Journal of Colloid and Interface Science}},
  publisher    = {{Elsevier BV}},
  title        = {{{Coil dimensions of macromolecules in the presence of crowding colloids: Impact of crowder size}}},
  doi          = {{10.1016/j.jcis.2025.137340}},
  volume       = {{691}},
  year         = {{2025}},
}

@article{62179,
  author       = {{Koch, Leon and Baier, Dominik and Rajput, Satyendra and König, Benedikt and Tiemann, Michael and Ebbinghaus, Simon and Nayar, Divya and Huber, Klaus}},
  issn         = {{1520-6106}},
  journal      = {{The Journal of Physical Chemistry B}},
  number       = {{40}},
  pages        = {{10213--10228}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Disaggregation at High Volume Exclusion: An “Overcrowding” Effect}}},
  doi          = {{10.1021/acs.jpcb.5c01245}},
  volume       = {{129}},
  year         = {{2025}},
}

@article{57620,
  author       = {{Zhang, Bingru and Martens, Kevin and Kneer, Luisa and Nguyen, Linh and Kempter, Susanne and Huber, Klaus and Kitzerow, Heinz-Siegfried}},
  issn         = {{1542-1406}},
  journal      = {{Molecular Crystals and Liquid Crystals}},
  pages        = {{1--9}},
  publisher    = {{Informa UK Limited}},
  title        = {{{Investigation of nano-rods fabricated by the DNA origami method using static and dynamic light scattering}}},
  doi          = {{10.1080/15421406.2024.2418067}},
  year         = {{2024}},
}

@article{62255,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Cellular stress and ageing involve an increase in crowding and aggregation of amylogenic proteins. We here investigate if crowding is the intrinsic cause of aggregation and utilise a previously established non-protein aggregation sensor, namely pseudoisocyanine chloride (PIC). PIC shows fibrillization in cells into a highly fluorescent J-aggregated state and is sensitive to crowding. Surprisingly, cell stress conditions stabilise the monomeric rather than the aggregated state of PIC both in the cytoplasm and in stress granules. Regarding the different physiochemical changes of the cytoplasm occurring upon cell stress, involving volume reduction, phase separation and solidification, the intrinsic crowding effect is not the key factor to drive associated self-assembly processes.</jats:p>}},
  author       = {{Pollak, Roland and Koch, Leon and König, Benedikt and Ribeiro, Sara S. and Samanta, Nirnay and Huber, Klaus and Ebbinghaus, Simon}},
  issn         = {{2399-3669}},
  journal      = {{Communications Chemistry}},
  number       = {{1}},
  publisher    = {{Springer Science and Business Media LLC}},
  title        = {{{Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor}}},
  doi          = {{10.1038/s42004-024-01315-y}},
  volume       = {{7}},
  year         = {{2024}},
}

@article{62252,
  author       = {{Alfano, Caterina and Fichou, Yann and Huber, Klaus and Weiss, Matthias and Spruijt, Evan and Ebbinghaus, Simon and De Luca, Giuseppe and Morando, Maria Agnese and Vetri, Valeria and Temussi, Piero Andrea and Pastore, Annalisa}},
  issn         = {{0009-2665}},
  journal      = {{Chemical Reviews}},
  number       = {{6}},
  pages        = {{3186--3219}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Molecular Crowding: The History and Development of a Scientific Paradigm}}},
  doi          = {{10.1021/acs.chemrev.3c00615}},
  volume       = {{124}},
  year         = {{2024}},
}

@article{62251,
  author       = {{Müller, Wenke and Sroka, Weronika and Schweins, Ralf and Nöcker, Bernd and Poon, Jia-Fei and Huber, Klaus}},
  issn         = {{0743-7463}},
  journal      = {{Langmuir}},
  number       = {{17}},
  pages        = {{8872--8885}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Impact of Additive Hydrophilicity on Mixed Dye-Nonionic Surfactant Micelles: Micelle Morphology and Dye Localization}}},
  doi          = {{10.1021/acs.langmuir.4c00012}},
  volume       = {{40}},
  year         = {{2024}},
}

@article{62250,
  author       = {{Saha, Sanjib and Büngeler, Anne and Hense, Dominik and Strube, Oliver I. and Huber, Klaus}},
  issn         = {{0743-7463}},
  journal      = {{Langmuir}},
  number       = {{8}},
  pages        = {{4152--4163}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{On the Mechanism of Self-Assembly of Fibrinogen in Thrombin-free Aqueous Solution}}},
  doi          = {{10.1021/acs.langmuir.3c03132}},
  volume       = {{40}},
  year         = {{2024}},
}

@article{62254,
  author       = {{Koch, Leon and Saha, Sanjib and Huber, Klaus}},
  issn         = {{1948-7185}},
  journal      = {{The Journal of Physical Chemistry Letters}},
  number       = {{39}},
  pages        = {{9987--9993}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Impact of Temperature on the Self-Assembly of Fibrinogen in Thrombin-Free Solutions}}},
  doi          = {{10.1021/acs.jpclett.4c02180}},
  volume       = {{15}},
  year         = {{2024}},
}

@article{62253,
  author       = {{Koch, Leon and Pollak, Roland and Ebbinghaus, Simon and Huber, Klaus}},
  issn         = {{0743-7463}},
  journal      = {{Langmuir}},
  number       = {{31}},
  pages        = {{16151--16159}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Early Stages of FUS Droplet Formation via Liquid–Liquid Phase Separation}}},
  doi          = {{10.1021/acs.langmuir.4c01243}},
  volume       = {{40}},
  year         = {{2024}},
}

@article{41649,
  author       = {{Büngeler, Anne and Kollmann, Fabian and Huber, Klaus and Strube, Oliver I.}},
  issn         = {{1525-7797}},
  journal      = {{Biomacromolecules}},
  keywords     = {{Materials Chemistry, Polymers and Plastics, Biomaterials, Bioengineering}},
  number       = {{3}},
  pages        = {{1020--1029}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Targeted Synthesis of the Type-A Particle Substructure from Enzymatically Produced Eumelanin}}},
  doi          = {{10.1021/acs.biomac.1c01390}},
  volume       = {{23}},
  year         = {{2022}},
}

@article{41817,
  abstract     = {{<jats:p>Pseudo isocyanine chloride monomers equilibrate with H-oligomers and, separated by a threshold, with H-oligomers and fiber-like J-aggregates. The mechanism and thermodynamics of J-aggregate formation is interpreted with the concept of chain growth.</jats:p>}},
  author       = {{Hämisch, Benjamin and Huber, Klaus}},
  issn         = {{1744-683X}},
  journal      = {{Soft Matter}},
  keywords     = {{Condensed Matter Physics, General Chemistry}},
  number       = {{35}},
  pages        = {{8140--8152}},
  publisher    = {{Royal Society of Chemistry (RSC)}},
  title        = {{{Mechanism and equilibrium thermodynamics of H- and J-aggregate formation from pseudo isocyanine chloride in water}}},
  doi          = {{10.1039/d1sm00979f}},
  volume       = {{17}},
  year         = {{2021}},
}

@article{41818,
  author       = {{Hense, Dominik and Büngeler, Anne and Kollmann, Fabian and Hanke, Marcel and Orive, Alejandro and Keller, Adrian and Grundmeier, Guido and Huber, Klaus and Strube, Oliver I.}},
  issn         = {{1525-7797}},
  journal      = {{Biomacromolecules}},
  keywords     = {{Materials Chemistry, Polymers and Plastics, Biomaterials, Bioengineering}},
  number       = {{10}},
  pages        = {{4084--4094}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Self-Assembled Fibrinogen Hydro- and Aerogels with Fibrin-like 3D Structures}}},
  doi          = {{10.1021/acs.biomac.1c00489}},
  volume       = {{22}},
  year         = {{2021}},
}

@article{41816,
  author       = {{Wagner, Maximilian and Krieger, Anja and Minameyer, Martin and Hämisch, Benjamin and Huber, Klaus and Drewello, Thomas and Gröhn, Franziska}},
  issn         = {{0024-9297}},
  journal      = {{Macromolecules}},
  keywords     = {{Materials Chemistry, Inorganic Chemistry, Polymers and Plastics, Organic Chemistry}},
  number       = {{6}},
  pages        = {{2899--2911}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Multiresponsive Polymer Nanoparticles Based on Disulfide Bonds}}},
  doi          = {{10.1021/acs.macromol.1c00299}},
  volume       = {{54}},
  year         = {{2021}},
}

@article{41815,
  author       = {{Hämisch, Benjamin and Pollak, Roland and Ebbinghaus, Simon and Huber, Klaus}},
  issn         = {{2570-4206}},
  journal      = {{ChemSystemsChem}},
  keywords     = {{General Earth and Planetary Sciences, General Environmental Science}},
  number       = {{3}},
  publisher    = {{Wiley}},
  title        = {{{Thermodynamic Analysis of the Self‐Assembly of Pseudo Isocyanine Chloride in the Presence of Crowding Agents}}},
  doi          = {{10.1002/syst.202000051}},
  volume       = {{3}},
  year         = {{2021}},
}

@article{41819,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Block copolymers were prepared with two anionic polyelectrolyte blocks: sodium polyacrylate (PA) and sodium polystyrene sulfonate (PSS), in order to investigate their phase behavior in aqueous solution in the presence of Ca<jats:sup>2+</jats:sup> cations. Depending on the concentration of polymer and Ca<jats:sup>2+</jats:sup> and on the ratio of the block lengths in the copolymer, spherical micelles were observed. Micelle formation arises from the specific interaction of Ca<jats:sup>2+</jats:sup> with the PA block only. An extensive small-angle scattering study was performed in order to unravel the structure and dimensions of the block copolymer micelles. Deuteration of the PA block enabled us to perform contrast variation experiments using small-angle neutron scattering at variable ratios of light and heavy water which were combined with information from small-angle X-ray scattering and dynamic light scattering.</jats:p>}},
  author       = {{Carl, Nico and Prévost, Sylvain and Schweins, Ralf and Huber, Klaus}},
  issn         = {{0303-402X}},
  journal      = {{Colloid and Polymer Science}},
  keywords     = {{Materials Chemistry, Colloid and Surface Chemistry, Polymers and Plastics, Physical and Theoretical Chemistry}},
  number       = {{7}},
  pages        = {{663--679}},
  publisher    = {{Springer Science and Business Media LLC}},
  title        = {{{Contrast variation of micelles composed of Ca2+ and block copolymers of two negatively charged polyelectrolytes}}},
  doi          = {{10.1007/s00396-019-04596-1}},
  volume       = {{298}},
  year         = {{2020}},
}

@article{41821,
  author       = {{Sistemich, Linda and Kutsch, Miriam and Hämisch, Benjamin and Zhang, Ping and Shydlovskyi, Sergii and Britzen-Laurent, Nathalie and Stürzl, Michael and Huber, Klaus and Herrmann, Christian}},
  issn         = {{0022-2836}},
  journal      = {{Journal of Molecular Biology}},
  keywords     = {{Molecular Biology, Structural Biology}},
  number       = {{7}},
  pages        = {{2164--2185}},
  publisher    = {{Elsevier BV}},
  title        = {{{The Molecular Mechanism of Polymer Formation of Farnesylated Human Guanylate-binding Protein 1}}},
  doi          = {{10.1016/j.jmb.2020.02.009}},
  volume       = {{432}},
  year         = {{2020}},
}

@article{41820,
  author       = {{Hämisch, Benjamin and Pollak, Roland and Ebbinghaus, Simon and Huber, Klaus}},
  issn         = {{0947-6539}},
  journal      = {{Chemistry – A European Journal}},
  keywords     = {{General Chemistry, Catalysis, Organic Chemistry}},
  number       = {{31}},
  pages        = {{7041--7050}},
  publisher    = {{Wiley}},
  title        = {{{Self‐Assembly of Pseudo‐Isocyanine Chloride as a Sensor for Macromolecular Crowding In Vitro and In Vivo}}},
  doi          = {{10.1002/chem.202000113}},
  volume       = {{26}},
  year         = {{2020}},
}