@article{32406,
  abstract     = {{<jats:p>The efficient loading of DNA nanostructures with intercalating or groove-binding drugs is an important prerequisite for various applications in drug delivery. However, unambiguous verification and quantification of successful drug loading...</jats:p>}},
  author       = {{Hanke, Marcel and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2040-3364}},
  journal      = {{Nanoscale}},
  keywords     = {{General Materials Science}},
  pages        = {{11552--11560}},
  publisher    = {{Royal Society of Chemistry (RSC)}},
  title        = {{{Direct visualization of the drug loading of single DNA origami nanostructures by AFM-IR nanospectroscopy}}},
  doi          = {{10.1039/d2nr02701a}},
  volume       = {{14}},
  year         = {{2022}},
}

@article{30103,
  author       = {{Huang, Jingyuan and Orive, Alejandro Gonzalez and Krüger, Jan Tobias and Hoyer, Kay-Peter and Keller, Adrian and Grundmeier, Guido}},
  issn         = {{0010-938X}},
  journal      = {{Corrosion Science}},
  keywords     = {{General Materials Science, General Chemical Engineering, General Chemistry}},
  pages        = {{110186}},
  publisher    = {{Elsevier BV}},
  title        = {{{Influence of proteins on the corrosion of a conventional and selective laser beam melted FeMn alloy in physiological electrolytes}}},
  doi          = {{10.1016/j.corsci.2022.110186}},
  volume       = {{200}},
  year         = {{2022}},
}

@article{41504,
  author       = {{Huang, Jingyuan and Gonzalez Orive, Alejandro and Krüger, Jan Tobias and Hoyer, Kay-Peter and Keller, Adrian and Grundmeier, Guido}},
  issn         = {{0010-938X}},
  journal      = {{Corrosion Science}},
  keywords     = {{General Materials Science, General Chemical Engineering, General Chemistry}},
  publisher    = {{Elsevier BV}},
  title        = {{{Influence of proteins on the corrosion of a conventional and selective laser beam melted FeMn alloy in physiological electrolytes}}},
  doi          = {{10.1016/j.corsci.2022.110186}},
  volume       = {{200}},
  year         = {{2022}},
}

@article{26011,
  author       = {{Hense, Dominik and Büngeler, Anne and Kollmann, Fabian and Hanke, Marcel and Orive, Alejandro and Keller, Adrian and Grundmeier, Guido and Huber, Klaus and Strube, Oliver I.}},
  issn         = {{1525-7797}},
  journal      = {{Biomacromolecules}},
  pages        = {{4084–4094}},
  title        = {{{Self-Assembled Fibrinogen Hydro- and Aerogels with Fibrin-like 3D Structures}}},
  doi          = {{10.1021/acs.biomac.1c00489}},
  volume       = {{22}},
  year         = {{2021}},
}

@article{26759,
  abstract     = {{<jats:p>Coatings of modified TiO2 nanoparticles (TiO2-m) have been shown to effectively and selectively trap non-adherent cancer cells, with an enormous potential for applications in photodynamic therapy (PDT). Leukemia cells have a remarkable affinity for TiO2-m coatings, adhering to the surface by membrane structures and exhibiting morphologic characteristics of amoeboid locomotion. However, the details of the cell–substrate interaction induced by the TiO2-m coating remain elusive. With the aim to obtain a better understanding of this phenomenon, leukemia cell adhesion to such coatings was characterized by atomic force microscopy (AFM) for short contact times up to 60 min. The cell and membrane morphological parameters mean cell height, contact area, cell volume, and membrane roughness were determined at different contact times. These results reveal cell expansion and contraction phases occurring during the initial stage of adhesion. Subsequently, the leukemic cells reach what appears to be a new resting state, characterized by pinning of the cell membrane by TiO2-m nanoparticle aggregates protruding from the coating surface.</jats:p>}},
  author       = {{Garcia Diosa, Jaime Andres and Gonzalez Orive, Alejandro and Grundmeier, Guido and Camargo Amado, Ruben Jesus and Keller, Adrian}},
  issn         = {{2076-3417}},
  journal      = {{Applied Sciences}},
  pages        = {{9898}},
  title        = {{{Morphological Dynamics of Leukemia Cells on TiO2 Nanoparticle Coatings Studied by AFM}}},
  doi          = {{10.3390/app11219898}},
  volume       = {{11}},
  year         = {{2021}},
}

@article{26985,
  author       = {{Garcia-Diosa, Jaime Andrés and Orive, Alejandro Gonzalez and Grundmeier, Guido and Keller, Adrian and Camargo-Amado, Rubén Jesús}},
  issn         = {{0257-8972}},
  journal      = {{Surface and Coatings Technology}},
  pages        = {{127823}},
  title        = {{{Influence of thickness, homogeneity, and morphology of TiO2-m nanoparticle coatings on cancer cell adhesion}}},
  doi          = {{10.1016/j.surfcoat.2021.127823}},
  year         = {{2021}},
}

@article{22636,
  abstract     = {{<jats:p>The effects that solid–liquid interfaces exert on the aggregation of proteins and peptides are of high relevance for various fields of basic and applied research, ranging from molecular biology and biomedicine to nanotechnology. While the influence of surface chemistry has received a lot of attention in this context, the role of surface topography has mostly been neglected so far. In this work, therefore, we investigate the aggregation of the type 2 diabetes-associated peptide hormone hIAPP in contact with flat and nanopatterned silicon oxide surfaces. The nanopatterned surfaces are produced by ion beam irradiation, resulting in well-defined anisotropic ripple patterns with heights and periodicities of about 1.5 and 30 nm, respectively. Using time-lapse atomic force microscopy, the morphology of the hIAPP aggregates is characterized quantitatively. Aggregation results in both amorphous aggregates and amyloid fibrils, with the presence of the nanopatterns leading to retarded fibrillization and stronger amorphous aggregation. This is attributed to structural differences in the amorphous aggregates formed at the nanopatterned surface, which result in a lower propensity for nucleating amyloid fibrillization. Our results demonstrate that nanoscale surface topography may modulate peptide and protein aggregation pathways in complex and intricate ways.</jats:p>}},
  author       = {{Hanke, Marcel and Yang, Yu and Ji, Yuxin and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1422-0067}},
  journal      = {{International Journal of Molecular Sciences}},
  pages        = {{5142}},
  title        = {{{Nanoscale Surface Topography Modulates hIAPP Aggregation Pathways at Solid–Liquid Interfaces}}},
  doi          = {{10.3390/ijms22105142}},
  volume       = {{22}},
  year         = {{2021}},
}

@article{22637,
  abstract     = {{<jats:title>Abstract</jats:title>
               <jats:p>Doxorubicin (DOX) is a common drug in cancer chemotherapy, and its high DNA-binding affinity can be harnessed in preparing DOX-loaded DNA nanostructures for targeted delivery and therapeutics. Although DOX has been widely studied, the existing literature of DOX-loaded DNA-carriers remains limited and incoherent. Here, based on an in-depth spectroscopic analysis, we characterize and optimize the DOX loading into different 2D and 3D scaffolded DNA origami nanostructures (DONs). In our experimental conditions, all DONs show similar DOX binding capacities (one DOX molecule per two to three base pairs), and the binding equilibrium is reached within seconds, remarkably faster than previously acknowledged. To characterize drug release profiles, DON degradation and DOX release from the complexes upon DNase I digestion was studied. For the employed DONs, the relative doses (DOX molecules released per unit time) may vary by two orders of magnitude depending on the DON superstructure. In addition, we identify DOX aggregation mechanisms and spectral changes linked to pH, magnesium, and DOX concentration. These features have been largely ignored in experimenting with DNA nanostructures, but are probably the major sources of the incoherence of the experimental results so far. Therefore, we believe this work can act as a guide to tailoring the release profiles and developing better drug delivery systems based on DNA-carriers.</jats:p>}},
  author       = {{Ijäs, Heini and Shen, Boxuan and Heuer-Jungemann, Amelie and Keller, Adrian and Kostiainen, Mauri A and Liedl, Tim and Ihalainen, Janne A and Linko, Veikko}},
  issn         = {{0305-1048}},
  journal      = {{Nucleic Acids Research}},
  pages        = {{3048--3062}},
  title        = {{{Unraveling the interaction between doxorubicin and DNA origami nanostructures for customizable chemotherapeutic drug release}}},
  doi          = {{10.1093/nar/gkab097}},
  volume       = {{49}},
  year         = {{2021}},
}

@article{22638,
  author       = {{Xin, Y and Shen, B and Kostiainen, MA and Grundmeier, Guido and Castro, M and Linko, V and Keller, Adrian}},
  issn         = {{0947-6539}},
  journal      = {{Chemistry – A European Journal}},
  number       = {{33}},
  pages        = {{8564--8571}},
  title        = {{{Scaling Up DNA Origami Lattice Assembly.}}},
  doi          = {{10.1002/chem.202100784}},
  volume       = {{27}},
  year         = {{2021}},
}

@article{22639,
  author       = {{Yang, Y and Knust, S and Schwiderek, S and Qin, Q and Yun, Q and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2079-4991}},
  journal      = {{Nanomaterials}},
  number       = {{2}},
  pages        = {{ 357 }},
  title        = {{{Protein Adsorption at Nanorough Titanium Oxide Surfaces: The Importance of Surface Statistical Parameters beyond Surface Roughness.}}},
  doi          = {{10.3390/nano11020357}},
  volume       = {{11}},
  year         = {{2021}},
}

@article{22640,
  author       = {{Piskunen, Petteri and Shen, Boxuan and Keller, Adrian and Toppari, J. Jussi and Kostiainen, Mauri A. and Linko, Veikko}},
  issn         = {{2574-0970}},
  journal      = {{ACS Applied Nano Materials}},
  pages        = {{529--538}},
  title        = {{{Biotemplated Lithography of Inorganic Nanostructures (BLIN) for Versatile Patterning of Functional Materials}}},
  doi          = {{10.1021/acsanm.0c02849}},
  volume       = {{4}},
  year         = {{2021}},
}

@article{22641,
  author       = {{Smith, DM and Keller, Adrian}},
  issn         = {{2699-9307}},
  journal      = {{Advanced NanoBiomed Research}},
  pages        = {{2000049}},
  title        = {{{DNA Nanostructures in the Fight Against Infectious Diseases.}}},
  doi          = {{10.1002/anbr.202000049}},
  volume       = {{1}},
  year         = {{2021}},
}

@article{22642,
  author       = {{Xin, Y and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2699-9307}},
  journal      = {{Advanced NanoBiomed Research}},
  number       = {{2}},
  pages        = {{2170023}},
  title        = {{{Adsorption of SARS-CoV-2 Spike Protein S1 at Oxide Surfaces Studied by High-Speed Atomic Force Microscopy.}}},
  doi          = {{10.1002/anbr.202170023}},
  volume       = {{1}},
  year         = {{2021}},
}

@article{22643,
  author       = {{Yang, Yu and Yu, Mingrui and Böke, Frederik and Qin, Qin and Hübner, René and Knust, Steffen and Schwiderek, Sabrina and Grundmeier, Guido and Fischer, Horst and Keller, Adrian}},
  issn         = {{0169-4332}},
  journal      = {{Applied Surface Science}},
  pages        = {{147671}},
  title        = {{{Effect of nanoscale surface topography on the adsorption of globular proteins}}},
  doi          = {{10.1016/j.apsusc.2020.147671}},
  volume       = {{535}},
  year         = {{2021}},
}

@article{22773,
  abstract     = {{<jats:p>Ion beam irradiation of solid surfaces may result in the self-organized formation of well-defined topographic nanopatterns. Depending on the irradiation conditions and the material properties, isotropic or anisotropic patterns of differently shaped features may be obtained. Most intriguingly, the periodicities of these patterns can be adjusted in the range between less than twenty and several hundred nanometers, which covers the dimensions of many cellular and extracellular features. However, even though ion beam nanopatterning has been studied for several decades and is nowadays widely employed in the fabrication of functional surfaces, it has found its way into the biomaterials field only recently. This review provides a brief overview of the basics of ion beam nanopatterning, emphasizes aspects of particular relevance for biomaterials applications, and summarizes a number of recent studies that investigated the effects of such nanopatterned surfaces on the adsorption of biomolecules and the response of adhering cells. Finally, promising future directions and potential translational challenges are identified.</jats:p>}},
  author       = {{Yang, Yu and Keller, Adrian}},
  issn         = {{2076-3417}},
  journal      = {{Applied Sciences}},
  pages        = {{6575}},
  title        = {{{Ion Beam Nanopatterning of Biomaterial Surfaces}}},
  doi          = {{10.3390/app11146575}},
  volume       = {{11}},
  year         = {{2021}},
}

@article{22926,
  abstract     = {{<jats:p>Implant-associated infections are an increasingly severe burden on healthcare systems worldwide and many research activities currently focus on inhibiting microbial colonization of biomedically relevant surfaces. To obtain molecular-level understanding of the involved processes and interactions, we investigate the adsorption of synthetic adhesin-like peptide sequences derived from the type IV pili of the Pseudomonas aeruginosa strains PAK and PAO at abiotic model surfaces, i.e., Au, SiO2, and oxidized Ti. These peptides correspond to the sequences of the receptor-binding domain 128–144 of the major pilin protein, which is known to facilitate P. aeruginosa adhesion at biotic and abiotic surfaces. Using quartz crystal microbalance with dissipation monitoring (QCM-D), we find that peptide adsorption is material- as well as strain-dependent. At the Au surface, PAO(128–144) shows drastically stronger adsorption than PAK(128–144), whereas adsorption of both peptides is markedly reduced at the oxide surfaces with less drastic differences between the two sequences. These observations suggest that peptide adsorption is influenced by not only the peptide sequence, but also peptide conformation. Our results furthermore highlight the importance of molecular-level investigations to understand and ultimately control microbial colonization of surfaces.</jats:p>}},
  author       = {{Yang, Yu and Schwiderek, Sabrina and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2673-8023}},
  journal      = {{Micro}},
  number       = {{1}},
  pages        = {{129--139}},
  title        = {{{Strain-Dependent Adsorption of Pseudomonas aeruginosa-Derived Adhesin-like Peptides at Abiotic Surfaces}}},
  doi          = {{10.3390/micro1010010}},
  volume       = {{1}},
  year         = {{2021}},
}

@article{23023,
  abstract     = {{<jats:p>DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.</jats:p>}},
  author       = {{Xin, Yang and Zargariantabrizi, Amir Ardalan and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  pages        = {{4798}},
  title        = {{{Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy}}},
  doi          = {{10.3390/molecules26164798}},
  volume       = {{26}},
  year         = {{2021}},
}

@article{22635,
  abstract     = {{Photodynamic therapy (PDT) using TiO2 nanoparticles has become an important alternative treatment for different types of cancer due to their high photocatalytic activity and high absorption of UV-A light. To potentiate this treatment, we have coated commercial glass plates with TiO2 nanoparticles prepared by the sol–gel method (TiO2-m), which exhibit a remarkable selectivity for the irreversible trapping of cancer cells. The physicochemical properties of the deposited TiO2-m nanoparticle coatings have been characterized by a number of complementary surface-analytical techniques and their interaction with leukemia and healthy blood cells were investigated. Scanning electron and atomic force microscopy verify the formation of a compact layer of TiO2-m nanoparticles. The particles are predominantly in the anatase phase and have hydroxyl-terminated surfaces as revealed by Raman, X-ray photoelectron, and infrared spectroscopy, as well as X-ray diffraction. We find that lymphoblastic leukemia cells adhere to the TiO2-m coating and undergo amoeboid-like migration, whereas lymphocytic cells show distinctly weaker interactions with the coating. This evidences the potential of this nanomaterial coating to selectively trap cancer cells and renders it a promising candidate for the development of future prototypes of PDT devices for the treatment of leukemia and other types of cancers with non-adherent cells.}},
  author       = {{Garcia Diosa, Jaime Andres and Gonzalez Orive, Alejandro and Weinberger, Christian and Schwiderek, Sabrina and Knust, Steffen and Tiemann, Michael and Grundmeier, Guido and Keller, Adrian and Camargo Amado, Ruben Jesus}},
  issn         = {{1552-4973}},
  journal      = {{Journal of Biomedical Materials Research Part B: Applied Biomaterials}},
  pages        = {{2142–2153}},
  title        = {{{TiO2 nanoparticle coatings on glass surfaces for the selective trapping of leukemia cells from peripheral blood}}},
  doi          = {{10.1002/jbm.b.34862}},
  volume       = {{109}},
  year         = {{2021}},
}

@article{22644,
  abstract     = {{<jats:p>The aggregation of human islet amyloid polypeptide (hIAPP) plays a major role in the pathogenesis of type 2 diabetes mellitus (T2DM), and numerous strategies for controlling hIAPP aggregation have been investigated so far. In particular, several organic and inorganic nanoparticles (NPs) have shown the potential to influence the aggregation of hIAPP and other amyloidogenic proteins and peptides. In addition to conventional NPs, DNA nanostructures are receiving more and more attention from the biomedical field. Therefore, in this work, we investigated the effects of two different DNA origami nanostructures on hIAPP aggregation. To this end, we employed in situ turbidity measurements and ex situ atomic force microscopy (AFM). The turbidity measurements revealed a retarding effect of the DNA nanostructures on hIAPP aggregation, while the AFM results showed the co-aggregation of hIAPP with the DNA origami nanostructures into hybrid peptide–DNA aggregates. We assume that this was caused by strong electrostatic interactions between the negatively charged DNA origami nanostructures and the positively charged peptide. Most intriguingly, the influence of the DNA origami nanostructures on hIAPP aggregation differed from that of genomic double-stranded DNA (dsDNA) and appeared to depend on DNA origami superstructure. DNA origami nanostructures may thus represent a novel route for modulating amyloid aggregation in vivo.</jats:p>}},
  author       = {{Hanke, Marcel and Gonzalez Orive, Alejandro and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2079-4991}},
  journal      = {{Nanomaterials}},
  pages        = {{2200}},
  title        = {{{Effect of DNA Origami Nanostructures on hIAPP Aggregation}}},
  doi          = {{10.3390/nano10112200}},
  volume       = {{10}},
  year         = {{2020}},
}

@article{22645,
  abstract     = {{<jats:p>Immobile Holliday junctions represent not only the most fundamental building block of structural DNA nanotechnology but are also of tremendous importance for the in vitro investigation of genetic recombination and epigenetics. Here, we present a detailed study on the room-temperature assembly of immobile Holliday junctions with the help of the single-strand annealing protein Redβ. Individual DNA single strands are initially coated with protein monomers and subsequently hybridized to form a rigid blunt-ended four-arm junction. We investigate the efficiency of this approach for different DNA/protein ratios, as well as for different DNA sequence lengths. Furthermore, we also evaluate the potential of Redβ to anneal sticky-end modified Holliday junctions into hierarchical assemblies. We demonstrate the Redβ-mediated annealing of Holliday junction dimers, multimers, and extended networks several microns in size. While these hybrid DNA–protein nanostructures may find applications in the crystallization of DNA–protein complexes, our work shows the great potential of Redβ to aid in the synthesis of functional DNA nanostructures under mild reaction conditions.</jats:p>}},
  author       = {{Ramakrishnan, Saminathan and Subramaniam, Sivaraman and Kielar, Charlotte and Grundmeier, Guido and Stewart, A. Francis and Keller, Adrian}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  pages        = {{5099}},
  title        = {{{Protein-Assisted Room-Temperature Assembly of Rigid, Immobile Holliday Junctions and Hierarchical DNA Nanostructures}}},
  doi          = {{10.3390/molecules25215099}},
  volume       = {{25}},
  year         = {{2020}},
}