@article{22646,
  abstract     = {{<jats:title>Abstract</jats:title>
<jats:p>The surface-assisted hierarchical self-assembly of DNA origami lattices represents a versatile and straightforward method for the organization of functional nanoscale objects such as proteins and nanoparticles. Here, we demonstrate that controlling the binding and exchange of different monovalent and divalent cation species at the DNA-mica interface enables the self-assembly of highly ordered DNA origami lattices on mica surfaces. The development of lattice quality and order is quantified by a detailed topological analysis of high-speed atomic force microscopy (HS-AFM) images. We find that lattice formation and quality strongly depend on the monovalent cation species. Na<jats:sup>+</jats:sup> is more effective than Li<jats:sup>+</jats:sup> and K<jats:sup>+</jats:sup> in facilitating the assembly of high-quality DNA origami lattices, because it is replacing the divalent cations at their binding sites in the DNA backbone more efficiently. With regard to divalent cations, Ca<jats:sup>2+</jats:sup> can be displaced more easily from the backbone phosphates than Mg<jats:sup>2+</jats:sup> and is thus superior in guiding lattice assembly. By independently adjusting incubation time, DNA origami concentration, and cation species, we thus obtain a highly ordered DNA origami lattice with an unprecedented normalized correlation length of 8.2. Beyond the correlation length, we use computer vision algorithms to compute the time course of different topological observables that, overall, demonstrate that replacing MgCl<jats:sub>2</jats:sub> by CaCl<jats:sub>2</jats:sub> enables the synthesis of DNA origami lattices with drastically increased lattice order.</jats:p>}},
  author       = {{Xin, Yang and Martinez Rivadeneira, Salvador and Grundmeier, Guido and Castro, Mario and Keller, Adrian}},
  issn         = {{1998-0124}},
  journal      = {{Nano Research}},
  pages        = {{3142--3150}},
  title        = {{{Self-assembly of highly ordered DNA origami lattices at solid-liquid interfaces by controlling cation binding and exchange}}},
  doi          = {{10.1007/s12274-020-2985-4}},
  volume       = {{13}},
  year         = {{2020}},
}

@article{22647,
  author       = {{Kielar, Charlotte and Zhu, Siqi and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1433-7851}},
  journal      = {{Angewandte Chemie International Edition}},
  pages        = {{14336--14341}},
  title        = {{{Quantitative Assessment of Tip Effects in Single‐Molecule High‐Speed Atomic Force Microscopy Using DNA Origami Substrates}}},
  doi          = {{10.1002/anie.202005884}},
  volume       = {{59}},
  year         = {{2020}},
}

@article{22648,
  abstract     = {{<p>DNA origami lattice formation at solid–liquid interfaces is surprisingly resilient toward the incorporation of DNA origami impurities with different shapes.</p>}},
  author       = {{Xin, Yang and Ji, Xueyin and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2040-3364}},
  journal      = {{Nanoscale}},
  pages        = {{9733--9743}},
  title        = {{{Dynamics of lattice defects in mixed DNA origami monolayers}}},
  doi          = {{10.1039/d0nr01252a}},
  volume       = {{12}},
  year         = {{2020}},
}

@article{22649,
  author       = {{Xin, Yang and Kielar, Charlotte and Zhu, Siqi and Sikeler, Christoph and Xu, Xiaodan and Möser, Christin and Grundmeier, Guido and Liedl, Tim and Heuer‐Jungemann, Amelie and Smith, David M. and Keller, Adrian}},
  issn         = {{1613-6810}},
  journal      = {{Small}},
  pages        = {{1905959}},
  title        = {{{Cryopreservation of DNA Origami Nanostructures}}},
  doi          = {{10.1002/smll.201905959}},
  volume       = {{16}},
  year         = {{2020}},
}

@article{22650,
  author       = {{Keller, Adrian and Linko, Veikko}},
  issn         = {{1433-7851}},
  journal      = {{Angewandte Chemie International Edition}},
  pages        = {{15818--15833}},
  title        = {{{Challenges and Perspectives of DNA Nanostructures in Biomedicine}}},
  doi          = {{10.1002/anie.201916390}},
  volume       = {{59}},
  year         = {{2020}},
}

@article{22651,
  author       = {{Keller, Adrian and Grundmeier, Guido}},
  issn         = {{0169-4332}},
  journal      = {{Applied Surface Science}},
  pages        = {{144991}},
  title        = {{{Amyloid aggregation at solid-liquid interfaces: Perspectives of studies using model surfaces}}},
  doi          = {{10.1016/j.apsusc.2019.144991}},
  volume       = {{506}},
  year         = {{2020}},
}

@article{22684,
  author       = {{Huang, Jingyuan and Suma, Antonio and Cui, Meiying and Grundmeier, Guido and Carnevale, Vincenzo and Zhang, Yixin and Kielar, Charlotte and Keller, Adrian}},
  issn         = {{2688-4062}},
  journal      = {{Small Structures}},
  pages        = {{2000038}},
  title        = {{{Arranging Small Molecules with Subnanometer Precision on DNA Origami Substrates for the Single‐Molecule Investigation of Protein–Ligand Interactions}}},
  doi          = {{10.1002/sstr.202000038}},
  volume       = {{1}},
  year         = {{2020}},
}

@article{22652,
  author       = {{Hämisch, Benjamin and Büngeler, Anne and Kielar, Charlotte and Keller, Adrian and Strube, Oliver and Huber, Klaus}},
  issn         = {{0743-7463}},
  journal      = {{Langmuir}},
  pages        = {{12113--12122}},
  title        = {{{Self-Assembly of Fibrinogen in Aqueous, Thrombin-Free Solutions of Variable Ionic Strengths}}},
  doi          = {{10.1021/acs.langmuir.9b01515}},
  volume       = {{35}},
  year         = {{2019}},
}

@article{22653,
  abstract     = {{<p>Merging of bridging staples with adjacent oligonucleotide sequences leads to a moderate increase of DNA origami stability, while enzymatic ligation after assembly yields a reinforced nanostructure with superior stability at up to 37 °C and in the presence of 6 M urea.</p>}},
  author       = {{Ramakrishnan, Saminathan and Schärfen, Leonard and Hunold, Kristin and Fricke, Sebastian and Grundmeier, Guido and Schlierf, Michael and Keller, Adrian and Krainer, Georg}},
  issn         = {{2040-3364}},
  journal      = {{Nanoscale}},
  pages        = {{16270--16276}},
  title        = {{{Enhancing the stability of DNA origami nanostructures: staple strand redesign versus enzymatic ligation}}},
  doi          = {{10.1039/c9nr04460d}},
  volume       = {{11}},
  year         = {{2019}},
}

@article{22654,
  abstract     = {{<jats:p>DNA origami nanostructures are widely employed in various areas of fundamental and applied research. Due to the tremendous success of the DNA origami technique in the academic field, considerable efforts currently aim at the translation of this technology from a laboratory setting to real-world applications, such as nanoelectronics, drug delivery, and biosensing. While many of these real-world applications rely on an intact DNA origami shape, they often also subject the DNA origami nanostructures to rather harsh and potentially damaging environmental and processing conditions. Furthermore, in the context of DNA origami mass production, the long-term storage of DNA origami nanostructures or their pre-assembled components also becomes an issue of high relevance, especially regarding the possible negative effects on DNA origami structural integrity. Thus, we investigated the effect of staple age on the self-assembly and stability of DNA origami nanostructures using atomic force microscopy. Different harsh processing conditions were simulated by applying different sample preparation protocols. Our results show that staple solutions may be stored at −20 °C for several years without impeding DNA origami self-assembly. Depending on DNA origami shape and superstructure, however, staple age may have negative effects on DNA origami stability under harsh treatment conditions. Mass spectrometry analysis of the aged staple mixtures revealed no signs of staple fragmentation. We, therefore, attribute the increased DNA origami sensitivity toward environmental conditions to an accumulation of damaged nucleobases, which undergo weaker base-pairing interactions and thus lead to reduced duplex stability.</jats:p>}},
  author       = {{Kielar, Charlotte and Xin, Yang and Xu, Xiaodan and Zhu, Siqi and Gorin, Nelli and Grundmeier, Guido and Möser, Christin and Smith, David M. and Keller, Adrian}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  pages        = {{2577}},
  title        = {{{Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability}}},
  doi          = {{10.3390/molecules24142577}},
  volume       = {{24}},
  year         = {{2019}},
}

@article{22655,
  author       = {{Ramakrishnan, S and Shen, B and Kostiainen, MA and Grundmeier, Guido and Keller, Adrian and Linko, V}},
  issn         = {{1439-4227}},
  journal      = {{ChemBioChem}},
  number       = {{22}},
  pages        = {{2818--2823}},
  title        = {{{Real-Time Observation of Superstructure-Dependent DNA Origami Digestion by DNase I Using High-Speed Atomic Force Microscopy.}}},
  doi          = {{10.1002/cbic.201900369}},
  volume       = {{20}},
  year         = {{2019}},
}

@article{22656,
  author       = {{Julin, S and Korpi, A and Shen, B and Liljeström, V and Ikkala, O and Keller, Adrian and Linko, V and Kostiainen, MA}},
  issn         = {{2040-3364}},
  journal      = {{Nanoscale}},
  number       = {{10}},
  pages        = {{4546--4551}},
  title        = {{{DNA origami directed 3D nanoparticle superlattice via electrostatic assembly.}}},
  doi          = {{10.1039/c8nr09844a}},
  volume       = {{11}},
  year         = {{2019}},
}

@article{22657,
  author       = {{Hajiraissi, Roozbeh and Hanke, Marcel and Gonzalez Orive, Alejandro and Duderija, Belma and Hofmann, Ulrike and Zhang, Yixin and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2470-1343}},
  journal      = {{ACS Omega}},
  pages        = {{2649--2660}},
  title        = {{{Effect of Terminal Modifications on the Adsorption and Assembly of hIAPP(20–29)}}},
  doi          = {{10.1021/acsomega.8b03028}},
  volume       = {{4}},
  year         = {{2019}},
}

@article{22658,
  author       = {{Kielar, Charlotte and Ramakrishnan, Saminathan and Fricke, Sebastian and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1944-8244}},
  journal      = {{ACS Applied Materials & Interfaces}},
  pages        = {{44844--44853}},
  title        = {{{Dynamics of DNA Origami Lattice Formation at Solid–Liquid Interfaces}}},
  doi          = {{10.1021/acsami.8b16047}},
  volume       = {{10}},
  year         = {{2018}},
}

@article{22659,
  author       = {{Ramakrishnan, Saminathan and Ijäs, Heini and Linko, Veikko and Keller, Adrian}},
  issn         = {{2001-0370}},
  journal      = {{Computational and Structural Biotechnology Journal}},
  pages        = {{342--349}},
  title        = {{{Structural stability of DNA origami nanostructures under application-specific conditions}}},
  doi          = {{10.1016/j.csbj.2018.09.002}},
  volume       = {{16}},
  year         = {{2018}},
}

@article{22660,
  author       = {{Kielar, Charlotte and Reddavide, Francesco V. and Tubbenhauer, Stefan and Cui, Meiying and Xu, Xiaodan and Grundmeier, Guido and Zhang, Yixin and Keller, Adrian}},
  issn         = {{1433-7851}},
  journal      = {{Angewandte Chemie International Edition}},
  pages        = {{14873--14877}},
  title        = {{{Pharmacophore Nanoarrays on DNA Origami Substrates as a Single-Molecule Assay for Fragment-Based Drug Discovery}}},
  doi          = {{10.1002/anie.201806778}},
  volume       = {{57}},
  year         = {{2018}},
}

@article{22661,
  author       = {{Kollmann, Fabian and Ramakrishnan, Saminathan and Shen, Boxuan and Grundmeier, Guido and Kostiainen, Mauri A. and Linko, Veikko and Keller, Adrian}},
  issn         = {{2470-1343}},
  journal      = {{ACS Omega}},
  pages        = {{9441--9448}},
  title        = {{{Superstructure-Dependent Loading of DNA Origami Nanostructures with a Groove-Binding Drug}}},
  doi          = {{10.1021/acsomega.8b00934}},
  volume       = {{3}},
  year         = {{2018}},
}

@inbook{22662,
  author       = {{Ramakrishnan, Saminathan and Grundmeier, Guido and Keller, Adrian}},
  booktitle    = {{DNA Nanotechnology: Methods and Protocols}},
  editor       = {{Zuccheri, Giampaolo }},
  isbn         = {{9781493985814}},
  issn         = {{1064-3745}},
  publisher    = {{Humana Press}},
  title        = {{{Directed Protein Adsorption Through DNA Origami Masks}}},
  doi          = {{10.1007/978-1-4939-8582-1_17}},
  volume       = {{1811}},
  year         = {{2018}},
}

@article{22663,
  author       = {{Kielar, Charlotte and Xin, Yang and Shen, Boxuan and Kostiainen, Mauri A. and Grundmeier, Guido and Linko, Veikko and Keller, Adrian}},
  issn         = {{1433-7851}},
  journal      = {{Angewandte Chemie International Edition}},
  pages        = {{9470--9474}},
  title        = {{{On the Stability of DNA Origami Nanostructures in Low-Magnesium Buffers}}},
  doi          = {{10.1002/anie.201802890}},
  volume       = {{57}},
  year         = {{2018}},
}

@article{22664,
  author       = {{Brassat, Katharina and Ramakrishnan, Saminathan and Bürger, Julius and Hanke, Marcel and Doostdar, Mahnaz and Lindner, Jörg and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{0743-7463}},
  journal      = {{Langmuir}},
  pages        = {{14757--14765}},
  title        = {{{On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays}}},
  doi          = {{10.1021/acs.langmuir.8b00793}},
  volume       = {{34}},
  year         = {{2018}},
}