@article{22697, author = {{Knust, Steffen and Ruhm, Lukas and Kuhlmann, Andreas and Meinderink, Dennis and Bürger, Julius and Lindner, Jörg K. N. and Arcos de Pedro, Maria Teresa and Grundmeier, Guido}}, issn = {{0377-0486}}, journal = {{Journal of Raman Spectroscopy}}, pages = {{1237--1245}}, title = {{{In situ backside Raman spectroscopy of zinc oxide nanorods in an atmospheric‐pressure dielectric barrier discharge plasma}}}, doi = {{10.1002/jrs.6123}}, year = {{2021}}, } @article{22773, abstract = {{Ion beam irradiation of solid surfaces may result in the self-organized formation of well-defined topographic nanopatterns. Depending on the irradiation conditions and the material properties, isotropic or anisotropic patterns of differently shaped features may be obtained. Most intriguingly, the periodicities of these patterns can be adjusted in the range between less than twenty and several hundred nanometers, which covers the dimensions of many cellular and extracellular features. However, even though ion beam nanopatterning has been studied for several decades and is nowadays widely employed in the fabrication of functional surfaces, it has found its way into the biomaterials field only recently. This review provides a brief overview of the basics of ion beam nanopatterning, emphasizes aspects of particular relevance for biomaterials applications, and summarizes a number of recent studies that investigated the effects of such nanopatterned surfaces on the adsorption of biomolecules and the response of adhering cells. Finally, promising future directions and potential translational challenges are identified.}}, author = {{Yang, Yu and Keller, Adrian}}, issn = {{2076-3417}}, journal = {{Applied Sciences}}, pages = {{6575}}, title = {{{Ion Beam Nanopatterning of Biomaterial Surfaces}}}, doi = {{10.3390/app11146575}}, volume = {{11}}, year = {{2021}}, } @article{22926, abstract = {{Implant-associated infections are an increasingly severe burden on healthcare systems worldwide and many research activities currently focus on inhibiting microbial colonization of biomedically relevant surfaces. To obtain molecular-level understanding of the involved processes and interactions, we investigate the adsorption of synthetic adhesin-like peptide sequences derived from the type IV pili of the Pseudomonas aeruginosa strains PAK and PAO at abiotic model surfaces, i.e., Au, SiO2, and oxidized Ti. These peptides correspond to the sequences of the receptor-binding domain 128–144 of the major pilin protein, which is known to facilitate P. aeruginosa adhesion at biotic and abiotic surfaces. Using quartz crystal microbalance with dissipation monitoring (QCM-D), we find that peptide adsorption is material- as well as strain-dependent. At the Au surface, PAO(128–144) shows drastically stronger adsorption than PAK(128–144), whereas adsorption of both peptides is markedly reduced at the oxide surfaces with less drastic differences between the two sequences. These observations suggest that peptide adsorption is influenced by not only the peptide sequence, but also peptide conformation. Our results furthermore highlight the importance of molecular-level investigations to understand and ultimately control microbial colonization of surfaces.}}, author = {{Yang, Yu and Schwiderek, Sabrina and Grundmeier, Guido and Keller, Adrian}}, issn = {{2673-8023}}, journal = {{Micro}}, number = {{1}}, pages = {{129--139}}, title = {{{Strain-Dependent Adsorption of Pseudomonas aeruginosa-Derived Adhesin-like Peptides at Abiotic Surfaces}}}, doi = {{10.3390/micro1010010}}, volume = {{1}}, year = {{2021}}, } @article{23023, abstract = {{DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.}}, author = {{Xin, Yang and Zargariantabrizi, Amir Ardalan and Grundmeier, Guido and Keller, Adrian}}, issn = {{1420-3049}}, journal = {{Molecules}}, pages = {{4798}}, title = {{{Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy}}}, doi = {{10.3390/molecules26164798}}, volume = {{26}}, year = {{2021}}, } @inproceedings{21717, author = {{Schmolke, Tobias and Teutenberg, Dominik and Meschut, Gerson and Meinderink, Dennis and Koch, Leon and Ebbert, Christoph and Grundmeier, Guido}}, location = {{Online Event}}, title = {{{Entwicklung einer Methode zur Bewertung einer stahlintensiven Mischbau-Klebverbindung eines Batteriegehäuses gegenüber mechanischer und medialer Belastung und Berücksichtigung der Interphasenstruktur}}}, year = {{2021}}, } @article{34647, author = {{Brögelmann, T and Bobzin, K and Grundmeier, Guido and de los Arcos, T and Kruppe, N C and Schwiderek, S and Carlet, M}}, issn = {{0022-3727}}, journal = {{Journal of Physics D: Applied Physics}}, keywords = {{Surfaces, Coatings and Films, Acoustics and Ultrasonics, Condensed Matter Physics, Electronic, Optical and Magnetic Materials}}, number = {{3}}, publisher = {{IOP Publishing}}, title = {{{Durability of nanolayer Ti–Al–O–N hard coatings under simulated polycarbonate melt processing conditions}}}, doi = {{10.1088/1361-6463/ac2e31}}, volume = {{55}}, year = {{2021}}, } @article{34645, author = {{Tripathi, Tripurari Sharan and Wilken, Martin and Hoppe, Christian and de los Arcos, Teresa and Grundmeier, Guido and Devi, Anjana and Karppinen, Maarit}}, issn = {{1438-1656}}, journal = {{Advanced Engineering Materials}}, keywords = {{Condensed Matter Physics, General Materials Science}}, number = {{10}}, publisher = {{Wiley}}, title = {{{Atomic Layer Deposition of Copper Metal Films from Cu(acac) 2 and Hydroquinone Reductant}}}, doi = {{10.1002/adem.202100446}}, volume = {{23}}, year = {{2021}}, } @article{22538, author = {{de los Arcos de Pedro, Maria Teresa and Müller, Hendrik and Wang, Fuzeng and Damerla, Varun Raj and Hoppe, Christian and Weinberger, Christian and Tiemann, Michael and Grundmeier, Guido}}, issn = {{0924-2031}}, journal = {{Vibrational Spectroscopy}}, title = {{{Review of infrared spectroscopy techniques for the determination of internal structure in thin SiO2 films}}}, doi = {{10.1016/j.vibspec.2021.103256}}, year = {{2021}}, } @article{22539, author = {{Bobzin, K. and Kalscheuer, C. and Grundmeier, G. and de los Arcos de Pedro, Maria Teresa and Schwiderek, S. and Carlet, M.}}, issn = {{0257-8972}}, journal = {{Surface and Coatings Technology}}, title = {{{Design of a TiAlON multilayer coating: Oxidation stability and deformation behavior}}}, doi = {{10.1016/j.surfcoat.2021.127417}}, year = {{2021}}, } @article{22535, author = {{Knust, Steffen and Ruhm, Lukas and Kuhlmann, Andreas and Meinderink, Dennis and Bürger, Julius and Lindner, Jörg K. N. and de los Arcos de Pedro, Maria Teresa and Grundmeier, Guido}}, issn = {{0377-0486}}, journal = {{Journal of Raman Spectroscopy}}, pages = {{1237--1245}}, title = {{{In situ backside Raman spectroscopy of zinc oxide nanorods in an atmospheric‐pressure dielectric barrier discharge plasma}}}, doi = {{10.1002/jrs.6123}}, year = {{2021}}, } @article{25897, abstract = {{A comparison of infrared spectroscopic analytical approaches was made in order to assess their applicability for internal structure characterization of SiO2 thin films. Markers for porosity and/or disorder based on the analysis of the asymmetric stretching absorption band of SiO2 between 900−1350 cm−1 were discussed. The shape of this band, which shows a well-defined LO–TO splitting, depends not only on the inherent characteristics of the film under analysis but also on the particular geometry of the IR experiment and the specific surface selection rules of the substrate. Three types of SiO2 thin films with clearly defined porosity ranging from dense films to mesoporous films were investigated by transmission (at different incidence angles), direct specular reflection (at different angles), and diffuse reflection. Two different types of substrate, metallic and semiconducting, were used. The combined effect of substrate and specific technique in the final shape of the band, was discussed, and the efficacy for their applicability to the determination of porosity in thin SiO2 films was critically evaluated.}}, author = {{de los Arcos, Teresa and Müller, Hendrik and Wang, Fuzeng and Damerla, Varun Raj and Hoppe, Christian and Weinberger, Christian and Tiemann, Michael and Grundmeier, Guido}}, issn = {{0924-2031}}, journal = {{Vibrational Spectroscopy}}, title = {{{Review of infrared spectroscopy techniques for the determination of internal structure in thin SiO2 films}}}, doi = {{10.1016/j.vibspec.2021.103256}}, year = {{2021}}, } @article{22635, abstract = {{Photodynamic therapy (PDT) using TiO2 nanoparticles has become an important alternative treatment for different types of cancer due to their high photocatalytic activity and high absorption of UV-A light. To potentiate this treatment, we have coated commercial glass plates with TiO2 nanoparticles prepared by the sol–gel method (TiO2-m), which exhibit a remarkable selectivity for the irreversible trapping of cancer cells. The physicochemical properties of the deposited TiO2-m nanoparticle coatings have been characterized by a number of complementary surface-analytical techniques and their interaction with leukemia and healthy blood cells were investigated. Scanning electron and atomic force microscopy verify the formation of a compact layer of TiO2-m nanoparticles. The particles are predominantly in the anatase phase and have hydroxyl-terminated surfaces as revealed by Raman, X-ray photoelectron, and infrared spectroscopy, as well as X-ray diffraction. We find that lymphoblastic leukemia cells adhere to the TiO2-m coating and undergo amoeboid-like migration, whereas lymphocytic cells show distinctly weaker interactions with the coating. This evidences the potential of this nanomaterial coating to selectively trap cancer cells and renders it a promising candidate for the development of future prototypes of PDT devices for the treatment of leukemia and other types of cancers with non-adherent cells.}}, author = {{Garcia Diosa, Jaime Andres and Gonzalez Orive, Alejandro and Weinberger, Christian and Schwiderek, Sabrina and Knust, Steffen and Tiemann, Michael and Grundmeier, Guido and Keller, Adrian and Camargo Amado, Ruben Jesus}}, issn = {{1552-4973}}, journal = {{Journal of Biomedical Materials Research Part B: Applied Biomaterials}}, pages = {{2142–2153}}, title = {{{TiO2 nanoparticle coatings on glass surfaces for the selective trapping of leukemia cells from peripheral blood}}}, doi = {{10.1002/jbm.b.34862}}, volume = {{109}}, year = {{2021}}, } @article{22859, author = {{Grothe, Richard and Striewe, Jan Andre and Meinderink, Dennis and Tröster, Thomas and Grundmeier, Guido}}, journal = {{The Journal of Adhesion}}, publisher = {{Taylor & Francis }}, title = {{{Enhanced corrosion resistance of adhesive/galvanised steel interfaces by nanocrystalline ZnO thin film deposition and molecular adhesion promoting films}}}, doi = {{10.1080/00218464.2021.1957676}}, year = {{2021}}, } @article{22644, abstract = {{The aggregation of human islet amyloid polypeptide (hIAPP) plays a major role in the pathogenesis of type 2 diabetes mellitus (T2DM), and numerous strategies for controlling hIAPP aggregation have been investigated so far. In particular, several organic and inorganic nanoparticles (NPs) have shown the potential to influence the aggregation of hIAPP and other amyloidogenic proteins and peptides. In addition to conventional NPs, DNA nanostructures are receiving more and more attention from the biomedical field. Therefore, in this work, we investigated the effects of two different DNA origami nanostructures on hIAPP aggregation. To this end, we employed in situ turbidity measurements and ex situ atomic force microscopy (AFM). The turbidity measurements revealed a retarding effect of the DNA nanostructures on hIAPP aggregation, while the AFM results showed the co-aggregation of hIAPP with the DNA origami nanostructures into hybrid peptide–DNA aggregates. We assume that this was caused by strong electrostatic interactions between the negatively charged DNA origami nanostructures and the positively charged peptide. Most intriguingly, the influence of the DNA origami nanostructures on hIAPP aggregation differed from that of genomic double-stranded DNA (dsDNA) and appeared to depend on DNA origami superstructure. DNA origami nanostructures may thus represent a novel route for modulating amyloid aggregation in vivo.}}, author = {{Hanke, Marcel and Gonzalez Orive, Alejandro and Grundmeier, Guido and Keller, Adrian}}, issn = {{2079-4991}}, journal = {{Nanomaterials}}, pages = {{2200}}, title = {{{Effect of DNA Origami Nanostructures on hIAPP Aggregation}}}, doi = {{10.3390/nano10112200}}, volume = {{10}}, year = {{2020}}, } @article{22645, abstract = {{Immobile Holliday junctions represent not only the most fundamental building block of structural DNA nanotechnology but are also of tremendous importance for the in vitro investigation of genetic recombination and epigenetics. Here, we present a detailed study on the room-temperature assembly of immobile Holliday junctions with the help of the single-strand annealing protein Redβ. Individual DNA single strands are initially coated with protein monomers and subsequently hybridized to form a rigid blunt-ended four-arm junction. We investigate the efficiency of this approach for different DNA/protein ratios, as well as for different DNA sequence lengths. Furthermore, we also evaluate the potential of Redβ to anneal sticky-end modified Holliday junctions into hierarchical assemblies. We demonstrate the Redβ-mediated annealing of Holliday junction dimers, multimers, and extended networks several microns in size. While these hybrid DNA–protein nanostructures may find applications in the crystallization of DNA–protein complexes, our work shows the great potential of Redβ to aid in the synthesis of functional DNA nanostructures under mild reaction conditions.}}, author = {{Ramakrishnan, Saminathan and Subramaniam, Sivaraman and Kielar, Charlotte and Grundmeier, Guido and Stewart, A. Francis and Keller, Adrian}}, issn = {{1420-3049}}, journal = {{Molecules}}, pages = {{5099}}, title = {{{Protein-Assisted Room-Temperature Assembly of Rigid, Immobile Holliday Junctions and Hierarchical DNA Nanostructures}}}, doi = {{10.3390/molecules25215099}}, volume = {{25}}, year = {{2020}}, } @article{22646, abstract = {{Abstract The surface-assisted hierarchical self-assembly of DNA origami lattices represents a versatile and straightforward method for the organization of functional nanoscale objects such as proteins and nanoparticles. Here, we demonstrate that controlling the binding and exchange of different monovalent and divalent cation species at the DNA-mica interface enables the self-assembly of highly ordered DNA origami lattices on mica surfaces. The development of lattice quality and order is quantified by a detailed topological analysis of high-speed atomic force microscopy (HS-AFM) images. We find that lattice formation and quality strongly depend on the monovalent cation species. Na+ is more effective than Li+ and K+ in facilitating the assembly of high-quality DNA origami lattices, because it is replacing the divalent cations at their binding sites in the DNA backbone more efficiently. With regard to divalent cations, Ca2+ can be displaced more easily from the backbone phosphates than Mg2+ and is thus superior in guiding lattice assembly. By independently adjusting incubation time, DNA origami concentration, and cation species, we thus obtain a highly ordered DNA origami lattice with an unprecedented normalized correlation length of 8.2. Beyond the correlation length, we use computer vision algorithms to compute the time course of different topological observables that, overall, demonstrate that replacing MgCl2 by CaCl2 enables the synthesis of DNA origami lattices with drastically increased lattice order.}}, author = {{Xin, Yang and Martinez Rivadeneira, Salvador and Grundmeier, Guido and Castro, Mario and Keller, Adrian}}, issn = {{1998-0124}}, journal = {{Nano Research}}, pages = {{3142--3150}}, title = {{{Self-assembly of highly ordered DNA origami lattices at solid-liquid interfaces by controlling cation binding and exchange}}}, doi = {{10.1007/s12274-020-2985-4}}, volume = {{13}}, year = {{2020}}, } @article{22647, author = {{Kielar, Charlotte and Zhu, Siqi and Grundmeier, Guido and Keller, Adrian}}, issn = {{1433-7851}}, journal = {{Angewandte Chemie International Edition}}, pages = {{14336--14341}}, title = {{{Quantitative Assessment of Tip Effects in Single‐Molecule High‐Speed Atomic Force Microscopy Using DNA Origami Substrates}}}, doi = {{10.1002/anie.202005884}}, volume = {{59}}, year = {{2020}}, } @article{22648, abstract = {{

DNA origami lattice formation at solid–liquid interfaces is surprisingly resilient toward the incorporation of DNA origami impurities with different shapes.

}}, author = {{Xin, Yang and Ji, Xueyin and Grundmeier, Guido and Keller, Adrian}}, issn = {{2040-3364}}, journal = {{Nanoscale}}, pages = {{9733--9743}}, title = {{{Dynamics of lattice defects in mixed DNA origami monolayers}}}, doi = {{10.1039/d0nr01252a}}, volume = {{12}}, year = {{2020}}, } @article{22649, author = {{Xin, Yang and Kielar, Charlotte and Zhu, Siqi and Sikeler, Christoph and Xu, Xiaodan and Möser, Christin and Grundmeier, Guido and Liedl, Tim and Heuer‐Jungemann, Amelie and Smith, David M. and Keller, Adrian}}, issn = {{1613-6810}}, journal = {{Small}}, pages = {{1905959}}, title = {{{Cryopreservation of DNA Origami Nanostructures}}}, doi = {{10.1002/smll.201905959}}, volume = {{16}}, year = {{2020}}, } @article{22650, author = {{Keller, Adrian and Linko, Veikko}}, issn = {{1433-7851}}, journal = {{Angewandte Chemie International Edition}}, pages = {{15818--15833}}, title = {{{Challenges and Perspectives of DNA Nanostructures in Biomedicine}}}, doi = {{10.1002/anie.201916390}}, volume = {{59}}, year = {{2020}}, }