[{"language":[{"iso":"eng"}],"keyword":["Ultra-high-performance liquid chromatography","ion mobility separation","mass spectrometry","LC-MS hyphenation","sequence-defined oligomers"],"article_type":"original","department":[{"_id":"163"}],"user_id":"94","_id":"32416","status":"public","abstract":[{"lang":"eng","text":"In recent years, sequence-defined oligomers (SDOs) gained increasing interest due to their perfectly controlled molecular structure, thus providing defined properties. In order to tune the properties, different functionalities need to be incorporated into the oligomers and the chain tacticity needs to be controlled. Beside the synthesis of SDOs, suitable methods need to be found to analyze the molecular structure. In this work, oligomers exhibiting an alternating or block-wise sequence of side chain functionalities were analyzed using a hyphenation of ultra-high-performance liquid chromatography and electrospray ionization mass spectrometry enhanced by ion mobility separation (IMS). Moieties in the side chains were varied according to polarity and bulkiness. Moreover, chain tacticity was varied. Drift times in the IMS cell and the corresponding collision cross section (CCS) values were shown to be individual parameters allowing the identification of SDOs, even in the case that SDO structures only differ in sequence or tacticity of side chain functionalities. Thus, a library of CCS values was obtained as reference used for the analysis of complex mixtures of SDOs."}],"publication":"International Journal of Polymer Analysis and Characterization","type":"journal_article","doi":"10.1080/1023666x.2022.2100968","title":"Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity","date_created":"2022-07-26T06:38:52Z","author":[{"first_name":"Marie-Theres","full_name":"Berg, Marie-Theres","last_name":"Berg"},{"last_name":"Herberg","id":"94","full_name":"Herberg, Artjom","first_name":"Artjom"},{"full_name":"Kuckling, Dirk","id":"287","last_name":"Kuckling","first_name":"Dirk"}],"date_updated":"2023-01-10T08:14:52Z","publisher":"Informa UK Limited","page":"1-12","citation":{"ama":"Berg M-T, Herberg A, Kuckling D. Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity. <i>International Journal of Polymer Analysis and Characterization</i>. Published online 2022:1-12. doi:<a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">10.1080/1023666x.2022.2100968</a>","chicago":"Berg, Marie-Theres, Artjom Herberg, and Dirk Kuckling. “Hyphenation of Ultra-High-Performance Liquid Chromatography and Ion Mobility Mass Spectrometry for the Analysis of Sequence-Defined Oligomers with Different Functionalities and Tacticity.” <i>International Journal of Polymer Analysis and Characterization</i>, 2022, 1–12. <a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">https://doi.org/10.1080/1023666x.2022.2100968</a>.","ieee":"M.-T. Berg, A. Herberg, and D. Kuckling, “Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity,” <i>International Journal of Polymer Analysis and Characterization</i>, pp. 1–12, 2022, doi: <a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">10.1080/1023666x.2022.2100968</a>.","apa":"Berg, M.-T., Herberg, A., &#38; Kuckling, D. (2022). Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity. <i>International Journal of Polymer Analysis and Characterization</i>, 1–12. <a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">https://doi.org/10.1080/1023666x.2022.2100968</a>","short":"M.-T. Berg, A. Herberg, D. Kuckling, International Journal of Polymer Analysis and Characterization (2022) 1–12.","mla":"Berg, Marie-Theres, et al. “Hyphenation of Ultra-High-Performance Liquid Chromatography and Ion Mobility Mass Spectrometry for the Analysis of Sequence-Defined Oligomers with Different Functionalities and Tacticity.” <i>International Journal of Polymer Analysis and Characterization</i>, Informa UK Limited, 2022, pp. 1–12, doi:<a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">10.1080/1023666x.2022.2100968</a>.","bibtex":"@article{Berg_Herberg_Kuckling_2022, title={Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity}, DOI={<a href=\"https://doi.org/10.1080/1023666x.2022.2100968\">10.1080/1023666x.2022.2100968</a>}, journal={International Journal of Polymer Analysis and Characterization}, publisher={Informa UK Limited}, author={Berg, Marie-Theres and Herberg, Artjom and Kuckling, Dirk}, year={2022}, pages={1–12} }"},"year":"2022","publication_identifier":{"issn":["1023-666X","1563-5341"]},"publication_status":"published"},{"keyword":["Size-exclusion chromatography","triple detection","branching analysis","star polymers","poly(N-isopropylacrylamide)","β-cyclodextrin"],"language":[{"iso":"eng"}],"_id":"32865","user_id":"94","department":[{"_id":"163"}],"abstract":[{"text":"For the first time, poly(N-isopropylacrylamide) (PNIPAAm) star polymers with a β-cyclodextrin core are characterized in detail by size-exclusion chromatography (SEC) with triple detection to experimentally verify the number of arms. A combination of a refractive index detector, multi-angle laser light scattering detector, and an online-viscosimeter was used for branching analysis. At first, the SEC system was calibrated and the detector setup was validated using linear polystyrene reference polymers. The applicability of the established triple detection SEC for branching analysis was shown by the analysis of two commercially available polystyrene star polymers. Due to the high molar masses of the star polymers, both the contraction ratio g and g′ could be determined independently, thus allowing the calculation of the viscosity shielding ratio ε. Finally, the branching analysis of the PNIPAAm star polymers could experimentally confirm the assumed arm number of up to 21 arms. Moreover, an increasingly compact molecular structure and the influence of the arm number on the viscosity shielding ratio could be shown.","lang":"eng"}],"status":"public","type":"journal_article","publication":"International Journal of Polymer Analysis and Characterization","title":"Branching analysis of β-cyclodextrin-based poly(<i>N</i>-isopropylacrylamide) star polymers using triple detection SEC","doi":"10.1080/1023666x.2022.2110133","publisher":"Informa UK Limited","date_updated":"2023-01-10T08:13:52Z","author":[{"first_name":"Artjom","last_name":"Herberg","id":"94","full_name":"Herberg, Artjom"},{"first_name":"Dirk","last_name":"Kuckling","full_name":"Kuckling, Dirk","id":"287"}],"date_created":"2022-08-17T06:28:55Z","year":"2022","citation":{"apa":"Herberg, A., &#38; Kuckling, D. (2022). Branching analysis of β-cyclodextrin-based poly(<i>N</i>-isopropylacrylamide) star polymers using triple detection SEC. <i>International Journal of Polymer Analysis and Characterization</i>, 1–19. <a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">https://doi.org/10.1080/1023666x.2022.2110133</a>","mla":"Herberg, Artjom, and Dirk Kuckling. “Branching Analysis of β-Cyclodextrin-Based Poly(<i>N</i>-Isopropylacrylamide) Star Polymers Using Triple Detection SEC.” <i>International Journal of Polymer Analysis and Characterization</i>, Informa UK Limited, 2022, pp. 1–19, doi:<a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">10.1080/1023666x.2022.2110133</a>.","bibtex":"@article{Herberg_Kuckling_2022, title={Branching analysis of β-cyclodextrin-based poly(<i>N</i>-isopropylacrylamide) star polymers using triple detection SEC}, DOI={<a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">10.1080/1023666x.2022.2110133</a>}, journal={International Journal of Polymer Analysis and Characterization}, publisher={Informa UK Limited}, author={Herberg, Artjom and Kuckling, Dirk}, year={2022}, pages={1–19} }","short":"A. Herberg, D. Kuckling, International Journal of Polymer Analysis and Characterization (2022) 1–19.","ama":"Herberg A, Kuckling D. Branching analysis of β-cyclodextrin-based poly(<i>N</i>-isopropylacrylamide) star polymers using triple detection SEC. <i>International Journal of Polymer Analysis and Characterization</i>. Published online 2022:1-19. doi:<a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">10.1080/1023666x.2022.2110133</a>","chicago":"Herberg, Artjom, and Dirk Kuckling. “Branching Analysis of β-Cyclodextrin-Based Poly(<i>N</i>-Isopropylacrylamide) Star Polymers Using Triple Detection SEC.” <i>International Journal of Polymer Analysis and Characterization</i>, 2022, 1–19. <a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">https://doi.org/10.1080/1023666x.2022.2110133</a>.","ieee":"A. Herberg and D. Kuckling, “Branching analysis of β-cyclodextrin-based poly(<i>N</i>-isopropylacrylamide) star polymers using triple detection SEC,” <i>International Journal of Polymer Analysis and Characterization</i>, pp. 1–19, 2022, doi: <a href=\"https://doi.org/10.1080/1023666x.2022.2110133\">10.1080/1023666x.2022.2110133</a>."},"page":"1-19","publication_status":"published","publication_identifier":{"issn":["1023-666X","1563-5341"]}}]