---
_id: '66092'
abstract:
- lang: eng
text: "\r\n DNA origami nanostructures (DONs) have promising
applications in biomedicine and biosensing, which often require their efficient
binding to target cells. By immobilizing the glycopeptide antibiotic vancomycin
on DONs, DON binding to Gram‐positive and Gram‐negative bacteria can be facilitated.
Here, we investigate how this multivalent binding is affected by the number and
arrangement of the vancomycin modifications on two‐dimensional DONs. We find that
for both Gram‐positive\r\n Bacillus subtilis\r\n
\ and Gram‐negative\r\n Escherichia
coli\r\n , binding increases with the number
of vancomycin modifications per DON. In general, binding to\r\n E.
coli\r\n is stronger than to\r\n B.
subtilis\r\n , which may be attributed to differences
in the architectures of the cell envelopes. Interestingly, for both bacteria,
the total number of vancomycin modifications appears to be more important than
their arrangement, as DONs with 18 vancomycin molecules on one side show similar
binding as DONs with 18 vancomycin molecules distributed over both sides. This
enables the attachment of multiple probe molecules to the vancomycin‐free side
of the DONs for enhancing detection efficiency without compromising binding affinity.
These results may thus provide guidelines for the design and synthesis of vancomycin‐modified
DONs for antimicrobial drug delivery and pathogen detection.\r\n "
article_number: e70436
author:
- first_name: Özge
full_name: Coşkuner Leineweber, Özge
last_name: Coşkuner Leineweber
- first_name: Ulrike
full_name: Hofmann, Ulrike
last_name: Hofmann
- first_name: Guido
full_name: Grundmeier, Guido
id: '194'
last_name: Grundmeier
- first_name: Yixin
full_name: Zhang, Yixin
last_name: Zhang
- first_name: Adrian Clemens
full_name: Keller, Adrian Clemens
id: '48864'
last_name: Keller
orcid: 0000-0001-7139-3110
citation:
ama: Coşkuner Leineweber Ö, Hofmann U, Grundmeier G, Zhang Y, Keller AC. Vancomycin‐Mediated
Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria.
ChemBioChem. 2026;27(13). doi:10.1002/cbic.70436
apa: Coşkuner Leineweber, Ö., Hofmann, U., Grundmeier, G., Zhang, Y., & Keller,
A. C. (2026). Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive
and Gram‐Negative Bacteria. ChemBioChem, 27(13), Article e70436.
https://doi.org/10.1002/cbic.70436
bibtex: '@article{Coşkuner Leineweber_Hofmann_Grundmeier_Zhang_Keller_2026, title={Vancomycin‐Mediated
Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria},
volume={27}, DOI={10.1002/cbic.70436},
number={13e70436}, journal={ChemBioChem}, publisher={Wiley}, author={Coşkuner
Leineweber, Özge and Hofmann, Ulrike and Grundmeier, Guido and Zhang, Yixin and
Keller, Adrian Clemens}, year={2026} }'
chicago: Coşkuner Leineweber, Özge, Ulrike Hofmann, Guido Grundmeier, Yixin Zhang,
and Adrian Clemens Keller. “Vancomycin‐Mediated Binding of DNA Origami Nanostructures
to Gram‐Positive and Gram‐Negative Bacteria.” ChemBioChem 27, no. 13 (2026).
https://doi.org/10.1002/cbic.70436.
ieee: 'Ö. Coşkuner Leineweber, U. Hofmann, G. Grundmeier, Y. Zhang, and A. C. Keller,
“Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and
Gram‐Negative Bacteria,” ChemBioChem, vol. 27, no. 13, Art. no. e70436,
2026, doi: 10.1002/cbic.70436.'
mla: Coşkuner Leineweber, Özge, et al. “Vancomycin‐Mediated Binding of DNA Origami
Nanostructures to Gram‐Positive and Gram‐Negative Bacteria.” ChemBioChem,
vol. 27, no. 13, e70436, Wiley, 2026, doi:10.1002/cbic.70436.
short: Ö. Coşkuner Leineweber, U. Hofmann, G. Grundmeier, Y. Zhang, A.C. Keller,
ChemBioChem 27 (2026).
date_created: 2026-06-30T12:44:05Z
date_updated: 2026-06-30T13:01:42Z
department:
- _id: '302'
doi: 10.1002/cbic.70436
intvolume: ' 27'
issue: '13'
language:
- iso: eng
publication: ChemBioChem
publication_identifier:
issn:
- 1439-4227
- 1439-7633
publication_status: published
publisher: Wiley
status: public
title: Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive
and Gram‐Negative Bacteria
type: journal_article
user_id: '48864'
volume: 27
year: '2026'
...
---
_id: '51121'
abstract:
- lang: eng
text: DNA origami nanostructures are a powerful tool in biomedicine and
can be used to combat drug‐resistant bacterial infections. However, the effect
of unmodified DNA origami nanostructures on bacteria is yet to be elucidated.
With the aim to obtain a better understanding of this phenomenon, the effect of
three DNA origami shapes, i.e., DNA origami triangles, six‐helix bundles (6HBs),
and 24‐helix bundles (24HBs), on the growth of Gram‐negative Escherichia coli
and Gram‐positive Bacillus subtilis is investigated. These results reveal that
while triangles and 24HBs can be used as a source of nutrients by E. coli and
thereby promote population growth, their effect is much smaller than that of genomic
single‐ and double‐stranded DNA. However, no effect on E. coli population growth
is observed for the 6HBs. On the other hand, B. subtilis does not show any significant
changes in population growth when cultured with the different DNA origami shapes
or genomic DNA. The detailed effect of DNA origami nanostructures on bacterial
growth thus depends on the competence signals and uptake mechanism of each bacterial
species, as well as the DNA origami shape. This should be considered in the development
of antimicrobial DNA origami nanostructures.
author:
- first_name: Jaime Andres
full_name: Garcia-Diosa, Jaime Andres
last_name: Garcia-Diosa
- first_name: Guido
full_name: Grundmeier, Guido
id: '194'
last_name: Grundmeier
- first_name: Adrian
full_name: Keller, Adrian
id: '48864'
last_name: Keller
orcid: 0000-0001-7139-3110
citation:
ama: Garcia-Diosa JA, Grundmeier G, Keller A. Effect of DNA Origami Nanostructures
on Bacterial Growth. ChemBioChem. Published online 2024. doi:10.1002/cbic.202400091
apa: Garcia-Diosa, J. A., Grundmeier, G., & Keller, A. (2024). Effect of DNA
Origami Nanostructures on Bacterial Growth. ChemBioChem. https://doi.org/10.1002/cbic.202400091
bibtex: '@article{Garcia-Diosa_Grundmeier_Keller_2024, title={Effect of DNA Origami
Nanostructures on Bacterial Growth}, DOI={10.1002/cbic.202400091},
journal={ChemBioChem}, publisher={Wiley}, author={Garcia-Diosa, Jaime Andres and
Grundmeier, Guido and Keller, Adrian}, year={2024} }'
chicago: Garcia-Diosa, Jaime Andres, Guido Grundmeier, and Adrian Keller. “Effect
of DNA Origami Nanostructures on Bacterial Growth.” ChemBioChem, 2024.
https://doi.org/10.1002/cbic.202400091.
ieee: 'J. A. Garcia-Diosa, G. Grundmeier, and A. Keller, “Effect of DNA Origami
Nanostructures on Bacterial Growth,” ChemBioChem, 2024, doi: 10.1002/cbic.202400091.'
mla: Garcia-Diosa, Jaime Andres, et al. “Effect of DNA Origami Nanostructures on
Bacterial Growth.” ChemBioChem, Wiley, 2024, doi:10.1002/cbic.202400091.
short: J.A. Garcia-Diosa, G. Grundmeier, A. Keller, ChemBioChem (2024).
date_created: 2024-02-03T12:41:16Z
date_updated: 2024-02-03T12:42:48Z
department:
- _id: '302'
doi: 10.1002/cbic.202400091
keyword:
- Organic Chemistry
- Molecular Biology
- Molecular Medicine
- Biochemistry
language:
- iso: eng
publication: ChemBioChem
publication_identifier:
issn:
- 1439-4227
- 1439-7633
publication_status: published
publisher: Wiley
status: public
title: Effect of DNA Origami Nanostructures on Bacterial Growth
type: journal_article
user_id: '48864'
year: '2024'
...
---
_id: '44503'
author:
- first_name: Marcel
full_name: Hanke, Marcel
last_name: Hanke
- first_name: Emilia
full_name: Tomm, Emilia
last_name: Tomm
- first_name: Guido
full_name: Grundmeier, Guido
id: '194'
last_name: Grundmeier
- first_name: Adrian
full_name: Keller, Adrian
id: '48864'
last_name: Keller
orcid: 0000-0001-7139-3110
citation:
ama: Hanke M, Tomm E, Grundmeier G, Keller A. Effect of Ionic Strength on the Thermal
Stability of DNA Origami Nanostructures. ChemBioChem. Published online
2023. doi:10.1002/cbic.202300338
apa: Hanke, M., Tomm, E., Grundmeier, G., & Keller, A. (2023). Effect of Ionic
Strength on the Thermal Stability of DNA Origami Nanostructures. ChemBioChem.
https://doi.org/10.1002/cbic.202300338
bibtex: '@article{Hanke_Tomm_Grundmeier_Keller_2023, title={Effect of Ionic Strength
on the Thermal Stability of DNA Origami Nanostructures}, DOI={10.1002/cbic.202300338},
journal={ChemBioChem}, publisher={Wiley}, author={Hanke, Marcel and Tomm, Emilia
and Grundmeier, Guido and Keller, Adrian}, year={2023} }'
chicago: Hanke, Marcel, Emilia Tomm, Guido Grundmeier, and Adrian Keller. “Effect
of Ionic Strength on the Thermal Stability of DNA Origami Nanostructures.” ChemBioChem,
2023. https://doi.org/10.1002/cbic.202300338.
ieee: 'M. Hanke, E. Tomm, G. Grundmeier, and A. Keller, “Effect of Ionic Strength
on the Thermal Stability of DNA Origami Nanostructures,” ChemBioChem, 2023,
doi: 10.1002/cbic.202300338.'
mla: Hanke, Marcel, et al. “Effect of Ionic Strength on the Thermal Stability of
DNA Origami Nanostructures.” ChemBioChem, Wiley, 2023, doi:10.1002/cbic.202300338.
short: M. Hanke, E. Tomm, G. Grundmeier, A. Keller, ChemBioChem (2023).
date_created: 2023-05-05T10:47:29Z
date_updated: 2023-05-05T10:48:00Z
department:
- _id: '302'
doi: 10.1002/cbic.202300338
keyword:
- Organic Chemistry
- Molecular Biology
- Molecular Medicine
- Biochemistry
language:
- iso: eng
publication: ChemBioChem
publication_identifier:
issn:
- 1439-4227
- 1439-7633
publication_status: published
publisher: Wiley
status: public
title: Effect of Ionic Strength on the Thermal Stability of DNA Origami Nanostructures
type: journal_article
user_id: '48864'
year: '2023'
...
---
_id: '22655'
author:
- first_name: S
full_name: Ramakrishnan, S
last_name: Ramakrishnan
- first_name: B
full_name: Shen, B
last_name: Shen
- first_name: MA
full_name: Kostiainen, MA
last_name: Kostiainen
- first_name: Guido
full_name: Grundmeier, Guido
id: '194'
last_name: Grundmeier
- first_name: Adrian
full_name: Keller, Adrian
id: '48864'
last_name: Keller
orcid: 0000-0001-7139-3110
- first_name: V
full_name: Linko, V
last_name: Linko
citation:
ama: Ramakrishnan S, Shen B, Kostiainen M, Grundmeier G, Keller A, Linko V. Real-Time
Observation of Superstructure-Dependent DNA Origami Digestion by DNase I Using
High-Speed Atomic Force Microscopy. ChemBioChem. 2019;20(22):2818-2823.
doi:10.1002/cbic.201900369
apa: Ramakrishnan, S., Shen, B., Kostiainen, M., Grundmeier, G., Keller, A., &
Linko, V. (2019). Real-Time Observation of Superstructure-Dependent DNA Origami
Digestion by DNase I Using High-Speed Atomic Force Microscopy. ChemBioChem,
20(22), 2818–2823. https://doi.org/10.1002/cbic.201900369
bibtex: '@article{Ramakrishnan_Shen_Kostiainen_Grundmeier_Keller_Linko_2019, title={Real-Time
Observation of Superstructure-Dependent DNA Origami Digestion by DNase I Using
High-Speed Atomic Force Microscopy.}, volume={20}, DOI={10.1002/cbic.201900369},
number={22}, journal={ChemBioChem}, author={Ramakrishnan, S and Shen, B and Kostiainen,
MA and Grundmeier, Guido and Keller, Adrian and Linko, V}, year={2019}, pages={2818–2823}
}'
chicago: 'Ramakrishnan, S, B Shen, MA Kostiainen, Guido Grundmeier, Adrian Keller,
and V Linko. “Real-Time Observation of Superstructure-Dependent DNA Origami Digestion
by DNase I Using High-Speed Atomic Force Microscopy.” ChemBioChem 20, no.
22 (2019): 2818–23. https://doi.org/10.1002/cbic.201900369.'
ieee: S. Ramakrishnan, B. Shen, M. Kostiainen, G. Grundmeier, A. Keller, and V.
Linko, “Real-Time Observation of Superstructure-Dependent DNA Origami Digestion
by DNase I Using High-Speed Atomic Force Microscopy.,” ChemBioChem, vol.
20, no. 22, pp. 2818–2823, 2019.
mla: Ramakrishnan, S., et al. “Real-Time Observation of Superstructure-Dependent
DNA Origami Digestion by DNase I Using High-Speed Atomic Force Microscopy.” ChemBioChem,
vol. 20, no. 22, 2019, pp. 2818–23, doi:10.1002/cbic.201900369.
short: S. Ramakrishnan, B. Shen, M. Kostiainen, G. Grundmeier, A. Keller, V. Linko,
ChemBioChem 20 (2019) 2818–2823.
date_created: 2021-07-08T12:14:23Z
date_updated: 2022-01-06T06:55:38Z
department:
- _id: '302'
doi: 10.1002/cbic.201900369
external_id:
pmid:
- '31163091'
intvolume: ' 20'
issue: '22'
language:
- iso: eng
page: 2818-2823
pmid: '1'
publication: ChemBioChem
publication_identifier:
issn:
- 1439-4227
- 1439-7633
status: public
title: Real-Time Observation of Superstructure-Dependent DNA Origami Digestion by
DNase I Using High-Speed Atomic Force Microscopy.
type: journal_article
user_id: '48864'
volume: 20
year: '2019'
...