---
_id: '33394'
abstract:
- lang: eng
text: The effectiveness of whey protein plus potassium bicarbonate-enriched
diet (WP+KHCO3) in mitigating disuse-induced changes in muscle
fiber oxidative capacity and capillarization was investigated in a 21-day crossover
design bed rest study. Ten healthy men (31 ± 6 yr) once received WP+KHCO3
and once received a standardized isocaloric diet. Muscle biopsies were taken 2
days before and during the 19th day of bed rest (BR) from the soleus (SOL) and
vastus lateralis (VL) muscle. Whole-body aerobic power (V̇o2 max),
muscle fatigue, and isometric strength of knee extensor and plantar flexor muscles
were monitored. Muscle fiber types and capillaries were identified by immunohistochemistry.
Fiber oxidative capacity was determined as the optical density (OD) at 660 nm
of succinate dehydrogenase (SDH)-stained sections. The product of fiber cross-sectional
area and SDH-OD (integrated SDH) indicated the maximal oxygen consumption of that
fiber. The maximal oxygen consumption supported by a capillary was calculated
as the integrated SDH in its supply area. BR reduced isometric strength of knee
extensor muscles ( P < 0.05), and the fiber oxidative capacity ( P < 0.001)
and V̇o2 max ( P = 0.042), but had no significant impact
on muscle capillarization or fatigue resistance of thigh muscles. The maximal
oxygen consumption supported by a capillary was reduced by 24% in SOL and 16%
in VL ( P < 0.001). WP+KHCO3 attenuated the disuse-induced
reduction in fiber oxidative capacity in both muscles ( P < 0.01). In conclusion,
following 19 days of bed rest, the decrement in fiber oxidative capacity is proportionally
larger than the loss of capillaries. WP+KHCO3 appears to
attenuate disuse-induced reductions in fiber oxidative capacity.
author:
- first_name: Alessandra
full_name: Bosutti, Alessandra
last_name: Bosutti
- first_name: Michele
full_name: Salanova, Michele
last_name: Salanova
- first_name: Dieter
full_name: Blottner, Dieter
last_name: Blottner
- first_name: Judith
full_name: Bühlmeier, Judith
id: '89838'
last_name: Bühlmeier
- first_name: Edwin
full_name: Mulder, Edwin
last_name: Mulder
- first_name: Jörn
full_name: Rittweger, Jörn
last_name: Rittweger
- first_name: Moi Hoon
full_name: Yap, Moi Hoon
last_name: Yap
- first_name: Bergita
full_name: Ganse, Bergita
last_name: Ganse
- first_name: Hans
full_name: Degens, Hans
last_name: Degens
citation:
ama: Bosutti A, Salanova M, Blottner D, et al. Whey protein with potassium bicarbonate
supplement attenuates the reduction in muscle oxidative capacity during 19 days
of bed rest. Journal of Applied Physiology. 2016;121(4):838-848. doi:10.1152/japplphysiol.00936.2015
apa: Bosutti, A., Salanova, M., Blottner, D., Bühlmeier, J., Mulder, E., Rittweger,
J., Yap, M. H., Ganse, B., & Degens, H. (2016). Whey protein with potassium
bicarbonate supplement attenuates the reduction in muscle oxidative capacity during
19 days of bed rest. Journal of Applied Physiology, 121(4), 838–848.
https://doi.org/10.1152/japplphysiol.00936.2015
bibtex: '@article{Bosutti_Salanova_Blottner_Bühlmeier_Mulder_Rittweger_Yap_Ganse_Degens_2016,
title={Whey protein with potassium bicarbonate supplement attenuates the reduction
in muscle oxidative capacity during 19 days of bed rest}, volume={121}, DOI={10.1152/japplphysiol.00936.2015},
number={4}, journal={Journal of Applied Physiology}, publisher={American Physiological
Society}, author={Bosutti, Alessandra and Salanova, Michele and Blottner, Dieter
and Bühlmeier, Judith and Mulder, Edwin and Rittweger, Jörn and Yap, Moi Hoon
and Ganse, Bergita and Degens, Hans}, year={2016}, pages={838–848} }'
chicago: 'Bosutti, Alessandra, Michele Salanova, Dieter Blottner, Judith Bühlmeier,
Edwin Mulder, Jörn Rittweger, Moi Hoon Yap, Bergita Ganse, and Hans Degens. “Whey
Protein with Potassium Bicarbonate Supplement Attenuates the Reduction in Muscle
Oxidative Capacity during 19 Days of Bed Rest.” Journal of Applied Physiology
121, no. 4 (2016): 838–48. https://doi.org/10.1152/japplphysiol.00936.2015.'
ieee: 'A. Bosutti et al., “Whey protein with potassium bicarbonate supplement
attenuates the reduction in muscle oxidative capacity during 19 days of bed rest,”
Journal of Applied Physiology, vol. 121, no. 4, pp. 838–848, 2016, doi:
10.1152/japplphysiol.00936.2015.'
mla: Bosutti, Alessandra, et al. “Whey Protein with Potassium Bicarbonate Supplement
Attenuates the Reduction in Muscle Oxidative Capacity during 19 Days of Bed Rest.”
Journal of Applied Physiology, vol. 121, no. 4, American Physiological
Society, 2016, pp. 838–48, doi:10.1152/japplphysiol.00936.2015.
short: A. Bosutti, M. Salanova, D. Blottner, J. Bühlmeier, E. Mulder, J. Rittweger,
M.H. Yap, B. Ganse, H. Degens, Journal of Applied Physiology 121 (2016) 838–848.
date_created: 2022-09-15T09:34:29Z
date_updated: 2022-09-15T09:46:06Z
doi: 10.1152/japplphysiol.00936.2015
extern: '1'
intvolume: ' 121'
issue: '4'
keyword:
- Physiology (medical)
- Physiology
language:
- iso: eng
page: 838-848
publication: Journal of Applied Physiology
publication_identifier:
issn:
- 8750-7587
- 1522-1601
publication_status: published
publisher: American Physiological Society
status: public
title: Whey protein with potassium bicarbonate supplement attenuates the reduction
in muscle oxidative capacity during 19 days of bed rest
type: journal_article
user_id: '89838'
volume: 121
year: '2016'
...
---
_id: '33400'
abstract:
- lang: eng
text: 'We examined, in immobilization, the effect of a diet high in sodium
chloride (NaCl) on bone markers, nitrogen balance, and acid-base status. Eight
healthy male test subjects participated in a 14-day head-down-tilt bed rest (HDBR)
study. During the bed rest period they received, in a randomized crossover design,
a high (7.7 meq Na+/kg body wt per day) and a low (0.7 meq
Na+/kg body wt per day) NaCl diet. As expected, 24-h excretion
of urinary calcium was significantly greater in the high-NaCl-intake HDBR phase
than in the low-NaCl-intake HDBR phase ( P < 0.001). High NaCl intake caused
a 43–50% greater excretion of the bone resorption markers COOH- (CTX) and NH2-
(NTX) terminal telopeptide of type I collagen in HDBR than low NaCl in HDBR (CTX/NTX:
P < 0.001). Serum concentrations of the bone formation markers bone-specific
alkaline phosphatase (bAP) and NH2-terminal propeptide of
type I procollagen (PINP) were identical in both NaCl intake phases. High NaCl
intake led to a more negative nitrogen balance in HDBR ( P < 0.001). Changes
were accompanied by increased serum chloride concentration ( P = 0.008), reduced
blood bicarbonate ( P = 0.017), and base excess ( P = 0.009) whereas net acid
excretion was lower during high than during low NaCl intake in immobilization
( P < 0.001). High NaCl intake during immobilization exacerbates disuse-induced
bone and muscle loss by causing further protein wasting and an increase in bone
resorption. Changes in the acid-base status, mainly caused by disturbances in
electrolyte metabolism, seem to determine NaCl-induced degradation processes.'
author:
- first_name: Petra
full_name: Frings-Meuthen, Petra
last_name: Frings-Meuthen
- first_name: Judith
full_name: Bühlmeier, Judith
id: '89838'
last_name: Bühlmeier
- first_name: Natalie
full_name: Baecker, Natalie
last_name: Baecker
- first_name: Peter
full_name: Stehle, Peter
last_name: Stehle
- first_name: Rolf
full_name: Fimmers, Rolf
last_name: Fimmers
- first_name: Francisca
full_name: May, Francisca
last_name: May
- first_name: Goetz
full_name: Kluge, Goetz
last_name: Kluge
- first_name: Martina
full_name: Heer, Martina
last_name: Heer
citation:
ama: Frings-Meuthen P, Bühlmeier J, Baecker N, et al. High sodium chloride intake
exacerbates immobilization-induced bone resorption and protein losses. Journal
of Applied Physiology. 2011;111(2):537-542. doi:10.1152/japplphysiol.00454.2011
apa: Frings-Meuthen, P., Bühlmeier, J., Baecker, N., Stehle, P., Fimmers, R., May,
F., Kluge, G., & Heer, M. (2011). High sodium chloride intake exacerbates
immobilization-induced bone resorption and protein losses. Journal of Applied
Physiology, 111(2), 537–542. https://doi.org/10.1152/japplphysiol.00454.2011
bibtex: '@article{Frings-Meuthen_Bühlmeier_Baecker_Stehle_Fimmers_May_Kluge_Heer_2011,
title={High sodium chloride intake exacerbates immobilization-induced bone resorption
and protein losses}, volume={111}, DOI={10.1152/japplphysiol.00454.2011},
number={2}, journal={Journal of Applied Physiology}, publisher={American Physiological
Society}, author={Frings-Meuthen, Petra and Bühlmeier, Judith and Baecker, Natalie
and Stehle, Peter and Fimmers, Rolf and May, Francisca and Kluge, Goetz and Heer,
Martina}, year={2011}, pages={537–542} }'
chicago: 'Frings-Meuthen, Petra, Judith Bühlmeier, Natalie Baecker, Peter Stehle,
Rolf Fimmers, Francisca May, Goetz Kluge, and Martina Heer. “High Sodium Chloride
Intake Exacerbates Immobilization-Induced Bone Resorption and Protein Losses.”
Journal of Applied Physiology 111, no. 2 (2011): 537–42. https://doi.org/10.1152/japplphysiol.00454.2011.'
ieee: 'P. Frings-Meuthen et al., “High sodium chloride intake exacerbates
immobilization-induced bone resorption and protein losses,” Journal of Applied
Physiology, vol. 111, no. 2, pp. 537–542, 2011, doi: 10.1152/japplphysiol.00454.2011.'
mla: Frings-Meuthen, Petra, et al. “High Sodium Chloride Intake Exacerbates Immobilization-Induced
Bone Resorption and Protein Losses.” Journal of Applied Physiology, vol.
111, no. 2, American Physiological Society, 2011, pp. 537–42, doi:10.1152/japplphysiol.00454.2011.
short: P. Frings-Meuthen, J. Bühlmeier, N. Baecker, P. Stehle, R. Fimmers, F. May,
G. Kluge, M. Heer, Journal of Applied Physiology 111 (2011) 537–542.
date_created: 2022-09-15T09:37:29Z
date_updated: 2022-09-15T09:43:45Z
doi: 10.1152/japplphysiol.00454.2011
extern: '1'
intvolume: ' 111'
issue: '2'
keyword:
- Physiology (medical)
- Physiology
language:
- iso: eng
page: 537-542
publication: Journal of Applied Physiology
publication_identifier:
issn:
- 8750-7587
- 1522-1601
publication_status: published
publisher: American Physiological Society
status: public
title: High sodium chloride intake exacerbates immobilization-induced bone resorption
and protein losses
type: journal_article
user_id: '89838'
volume: 111
year: '2011'
...