[{"article_number":"e93581","language":[{"iso":"eng"}],"_id":"27768","doi":"10.1371/journal.pone.0093581","user_id":"61597","year":"2014","title":"Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts","status":"public","publication_identifier":{"issn":["1932-6203"]},"author":[{"full_name":"Riedel, Christina","first_name":"Christina","last_name":"Riedel"},{"full_name":"von Kries, Rüdiger","last_name":"von Kries","first_name":"Rüdiger"},{"full_name":"Buyken, Anette","first_name":"Anette","last_name":"Buyken","id":"65985"},{"full_name":"Diethelm, Katharina","last_name":"Diethelm","first_name":"Katharina"},{"full_name":"Keil, Thomas","first_name":"Thomas","last_name":"Keil"},{"full_name":"Grabenhenrich, Linus","last_name":"Grabenhenrich","first_name":"Linus"},{"full_name":"Müller, Manfred J.","first_name":"Manfred J.","last_name":"Müller"},{"full_name":"Plachta-Danielzik, Sandra","last_name":"Plachta-Danielzik","first_name":"Sandra"}],"date_updated":"2022-01-06T06:57:44Z","publication_status":"published","date_created":"2021-11-23T16:57:26Z","type":"journal_article","department":[{"_id":"17"},{"_id":"22"},{"_id":"571"}],"publication":"PLoS ONE","citation":{"ama":"Riedel C, von Kries R, Buyken A, et al. Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts. <i>PLoS ONE</i>. Published online 2014. doi:<a href=\"https://doi.org/10.1371/journal.pone.0093581\">10.1371/journal.pone.0093581</a>","short":"C. Riedel, R. von Kries, A. Buyken, K. Diethelm, T. Keil, L. Grabenhenrich, M.J. Müller, S. Plachta-Danielzik, PLoS ONE (2014).","chicago":"Riedel, Christina, Rüdiger von Kries, Anette Buyken, Katharina Diethelm, Thomas Keil, Linus Grabenhenrich, Manfred J. Müller, and Sandra Plachta-Danielzik. “Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts.” <i>PLoS ONE</i>, 2014. <a href=\"https://doi.org/10.1371/journal.pone.0093581\">https://doi.org/10.1371/journal.pone.0093581</a>.","bibtex":"@article{Riedel_von Kries_Buyken_Diethelm_Keil_Grabenhenrich_Müller_Plachta-Danielzik_2014, title={Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0093581\">10.1371/journal.pone.0093581</a>}, number={e93581}, journal={PLoS ONE}, author={Riedel, Christina and von Kries, Rüdiger and Buyken, Anette and Diethelm, Katharina and Keil, Thomas and Grabenhenrich, Linus and Müller, Manfred J. and Plachta-Danielzik, Sandra}, year={2014} }","apa":"Riedel, C., von Kries, R., Buyken, A., Diethelm, K., Keil, T., Grabenhenrich, L., Müller, M. J., &#38; Plachta-Danielzik, S. (2014). Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts. <i>PLoS ONE</i>, Article e93581. <a href=\"https://doi.org/10.1371/journal.pone.0093581\">https://doi.org/10.1371/journal.pone.0093581</a>","mla":"Riedel, Christina, et al. “Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts.” <i>PLoS ONE</i>, e93581, 2014, doi:<a href=\"https://doi.org/10.1371/journal.pone.0093581\">10.1371/journal.pone.0093581</a>.","ieee":"C. Riedel <i>et al.</i>, “Overweight in Adolescence Can Be Predicted at Age 6 Years: A CART Analysis in German Cohorts,” <i>PLoS ONE</i>, Art. no. e93581, 2014, doi: <a href=\"https://doi.org/10.1371/journal.pone.0093581\">10.1371/journal.pone.0093581</a>."}},{"date_created":"2020-06-24T13:01:10Z","type":"journal_article","department":[{"_id":"749"}],"publication":"PLoS ONE","issue":"3","citation":{"mla":"Vollmer, Anna-Lisa, et al. “Robots Show Us How to Teach Them: Feedback from Robots Shapes Tutoring Behavior during Action Learning.” <i>PLoS ONE</i>, vol. 9, no. 3, Public Library of Science (PLoS), 2014, doi:<a href=\"https://doi.org/10.1371/journal.pone.0091349\">10.1371/journal.pone.0091349</a>.","ama":"Vollmer A-L, Mühlig M, Steil JJ, et al. Robots show us how to teach them: Feedback from robots shapes tutoring behavior during action learning. <i>PLoS ONE</i>. 2014;9(3). doi:<a href=\"https://doi.org/10.1371/journal.pone.0091349\">10.1371/journal.pone.0091349</a>","bibtex":"@article{Vollmer_Mühlig_Steil_Pitsch_Fritsch_Rohlfing_Wrede_2014, title={Robots show us how to teach them: Feedback from robots shapes tutoring behavior during action learning}, volume={9}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0091349\">10.1371/journal.pone.0091349</a>}, number={3}, journal={PLoS ONE}, publisher={Public Library of Science (PLoS)}, author={Vollmer, Anna-Lisa and Mühlig, Manuel and Steil, Jochen J. and Pitsch, Karola and Fritsch, Jannik and Rohlfing, Katharina and Wrede, Britta}, year={2014} }","apa":"Vollmer, A.-L., Mühlig, M., Steil, J. J., Pitsch, K., Fritsch, J., Rohlfing, K., &#38; Wrede, B. (2014). Robots show us how to teach them: Feedback from robots shapes tutoring behavior during action learning. <i>PLoS ONE</i>, <i>9</i>(3). <a href=\"https://doi.org/10.1371/journal.pone.0091349\">https://doi.org/10.1371/journal.pone.0091349</a>","ieee":"A.-L. Vollmer <i>et al.</i>, “Robots show us how to teach them: Feedback from robots shapes tutoring behavior during action learning,” <i>PLoS ONE</i>, vol. 9, no. 3, 2014, doi: <a href=\"https://doi.org/10.1371/journal.pone.0091349\">10.1371/journal.pone.0091349</a>.","short":"A.-L. Vollmer, M. Mühlig, J.J. Steil, K. Pitsch, J. Fritsch, K. Rohlfing, B. Wrede, PLoS ONE 9 (2014).","chicago":"Vollmer, Anna-Lisa, Manuel Mühlig, Jochen J. Steil, Karola Pitsch, Jannik Fritsch, Katharina Rohlfing, and Britta Wrede. “Robots Show Us How to Teach Them: Feedback from Robots Shapes Tutoring Behavior during Action Learning.” <i>PLoS ONE</i> 9, no. 3 (2014). <a href=\"https://doi.org/10.1371/journal.pone.0091349\">https://doi.org/10.1371/journal.pone.0091349</a>."},"abstract":[{"text":"Robot learning by imitation requires the detection of a tutor's action demonstration and its relevant parts. Current approaches implicitly assume a unidirectional transfer of knowledge from tutor to learner. The presented work challenges this predominant assumption based on an extensive user study with an autonomously interacting robot. We show that by providing feedback, a robot learner influences the human tutor's movement demonstrations in the process of action learning. We argue that the robot's feedback strongly shapes how tutors signal what is relevant to an action and thus advocate a paradigm shift in robot action learning research toward truly interactive systems learning in and benefiting from interaction.","lang":"eng"}],"_id":"17193","publisher":"Public Library of Science (PLoS)","language":[{"iso":"eng"}],"doi":"10.1371/journal.pone.0091349","user_id":"14931","volume":9,"title":"Robots show us how to teach them: Feedback from robots shapes tutoring behavior during action learning","year":"2014","status":"public","author":[{"last_name":"Vollmer","first_name":"Anna-Lisa","full_name":"Vollmer, Anna-Lisa"},{"first_name":"Manuel","last_name":"Mühlig","full_name":"Mühlig, Manuel"},{"full_name":"Steil, Jochen J.","last_name":"Steil","first_name":"Jochen J."},{"full_name":"Pitsch, Karola","first_name":"Karola","last_name":"Pitsch"},{"last_name":"Fritsch","first_name":"Jannik","full_name":"Fritsch, Jannik"},{"first_name":"Katharina","last_name":"Rohlfing","full_name":"Rohlfing, Katharina","id":"50352"},{"first_name":"Britta","last_name":"Wrede","full_name":"Wrede, Britta"}],"publication_identifier":{"issn":["1932-6203"]},"date_updated":"2023-02-01T16:08:53Z","intvolume":"         9"},{"publication":"PLoS ONE","citation":{"bibtex":"@article{Günther_Walz_Kroke_Wudy_Riedel_von Kries_Joslowski_Remer_Cheng_Buyken_2013, title={Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0079436\">10.1371/journal.pone.0079436</a>}, number={e79436}, journal={PLoS ONE}, author={Günther, Anke L. B. and Walz, Helena and Kroke, Anja and Wudy, Stefan A. and Riedel, Christina and von Kries, Rüdiger and Joslowski, Gesa and Remer, Thomas and Cheng, Guo and Buyken, Anette}, year={2013} }","ama":"Günther ALB, Walz H, Kroke A, et al. Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis. <i>PLoS ONE</i>. Published online 2013. doi:<a href=\"https://doi.org/10.1371/journal.pone.0079436\">10.1371/journal.pone.0079436</a>","mla":"Günther, Anke L. B., et al. “Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis.” <i>PLoS ONE</i>, e79436, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0079436\">10.1371/journal.pone.0079436</a>.","short":"A.L.B. Günther, H. Walz, A. Kroke, S.A. Wudy, C. Riedel, R. von Kries, G. Joslowski, T. Remer, G. Cheng, A. Buyken, PLoS ONE (2013).","chicago":"Günther, Anke L. B., Helena Walz, Anja Kroke, Stefan A. Wudy, Christina Riedel, Rüdiger von Kries, Gesa Joslowski, Thomas Remer, Guo Cheng, and Anette Buyken. “Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis.” <i>PLoS ONE</i>, 2013. <a href=\"https://doi.org/10.1371/journal.pone.0079436\">https://doi.org/10.1371/journal.pone.0079436</a>.","ieee":"A. L. B. Günther <i>et al.</i>, “Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis,” <i>PLoS ONE</i>, Art. no. e79436, 2013, doi: <a href=\"https://doi.org/10.1371/journal.pone.0079436\">10.1371/journal.pone.0079436</a>.","apa":"Günther, A. L. B., Walz, H., Kroke, A., Wudy, S. A., Riedel, C., von Kries, R., Joslowski, G., Remer, T., Cheng, G., &#38; Buyken, A. (2013). Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis. <i>PLoS ONE</i>, Article e79436. <a href=\"https://doi.org/10.1371/journal.pone.0079436\">https://doi.org/10.1371/journal.pone.0079436</a>"},"type":"journal_article","department":[{"_id":"17"},{"_id":"22"},{"_id":"571"}],"date_created":"2021-11-23T16:02:57Z","date_updated":"2022-01-06T06:57:44Z","publication_status":"published","title":"Breastfeeding and Its Prospective Association with Components of the GH-IGF-Axis, Insulin Resistance and Body Adiposity Measures in Young Adulthood – Insights from Linear and Quantile Regression Analysis","year":"2013","status":"public","publication_identifier":{"issn":["1932-6203"]},"author":[{"full_name":"Günther, Anke L. B.","first_name":"Anke L. B.","last_name":"Günther"},{"full_name":"Walz, Helena","first_name":"Helena","last_name":"Walz"},{"full_name":"Kroke, Anja","first_name":"Anja","last_name":"Kroke"},{"full_name":"Wudy, Stefan A.","first_name":"Stefan A.","last_name":"Wudy"},{"last_name":"Riedel","first_name":"Christina","full_name":"Riedel, Christina"},{"full_name":"von Kries, Rüdiger","first_name":"Rüdiger","last_name":"von Kries"},{"full_name":"Joslowski, Gesa","last_name":"Joslowski","first_name":"Gesa"},{"full_name":"Remer, Thomas","first_name":"Thomas","last_name":"Remer"},{"full_name":"Cheng, Guo","last_name":"Cheng","first_name":"Guo"},{"first_name":"Anette","last_name":"Buyken","full_name":"Buyken, Anette","id":"65985"}],"doi":"10.1371/journal.pone.0079436","user_id":"61597","article_number":"e79436","_id":"27733","language":[{"iso":"eng"}]},{"doi":"10.1371/journal.pone.0054257","language":[{"iso":"eng"}],"article_number":"e54257","main_file_link":[{"url":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0054257","open_access":"1"}],"intvolume":"         8","publication_status":"published","date_updated":"2022-06-07T00:13:12Z","publication_identifier":{"issn":["1932-6203"]},"author":[{"first_name":"Katharina","last_name":"Weiß","full_name":"Weiß, Katharina"},{"full_name":"Hilkenmeier, Frederic","first_name":"Frederic","last_name":"Hilkenmeier"},{"id":"451","full_name":"Scharlau, Ingrid","first_name":"Ingrid","last_name":"Scharlau","orcid":"0000-0003-2364-9489"}],"year":"2013","title":"Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli","department":[{"_id":"424"}],"type":"journal_article","date_created":"2021-12-13T10:02:05Z","abstract":[{"text":"Why are nearly simultaneous stimuli frequently perceived in reversed order? The origin of errors in temporal judgments is a question older than experimental psychology itself. One of the earliest suspects is attention. According to the concept of prior entry, attention accelerates attended stimuli; thus they have “prior entry” to perceptive processing stages, including consciousness. Although latency advantages for attended stimuli have been revealed in psychophysical studies many times, these measures (e.g. temporal order judgments, simultaneity judgments) cannot test the prior-entry hypothesis completely. Since they assess latency differences between an attended and an unattended stimulus, they cannot distinguish between faster processing of attended stimuli and slower processing of unattended stimuli. Therefore, we present a novel paradigm providing separate estimates for processing advantages respectively disadvantages of attended and unattended stimuli. We found that deceleration of unattended stimuli contributes more strongly to the prior-entry illusion than acceleration of attended stimuli. Thus, in the temporal domain, attention fulfills its selective function primarily by deceleration of unattended stimuli. That means it is actually posterior entry, not prior entry which accounts for the largest part of the effect.","lang":"eng"}],"publication":"PLoS ONE","volume":8,"user_id":"42165","funded_apc":"1","_id":"28688","status":"public","oa":"1","citation":{"ama":"Weiß K, Hilkenmeier F, Scharlau I. Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli. <i>PLoS ONE</i>. 2013;8. doi:<a href=\"https://doi.org/10.1371/journal.pone.0054257\">10.1371/journal.pone.0054257</a>","bibtex":"@article{Weiß_Hilkenmeier_Scharlau_2013, title={Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli}, volume={8}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0054257\">10.1371/journal.pone.0054257</a>}, number={e54257}, journal={PLoS ONE}, author={Weiß, Katharina and Hilkenmeier, Frederic and Scharlau, Ingrid}, year={2013} }","mla":"Weiß, Katharina, et al. “Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli.” <i>PLoS ONE</i>, vol. 8, e54257, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0054257\">10.1371/journal.pone.0054257</a>.","short":"K. Weiß, F. Hilkenmeier, I. Scharlau, PLoS ONE 8 (2013).","chicago":"Weiß, Katharina, Frederic Hilkenmeier, and Ingrid Scharlau. “Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli.” <i>PLoS ONE</i> 8 (2013). <a href=\"https://doi.org/10.1371/journal.pone.0054257\">https://doi.org/10.1371/journal.pone.0054257</a>.","apa":"Weiß, K., Hilkenmeier, F., &#38; Scharlau, I. (2013). Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli. <i>PLoS ONE</i>, <i>8</i>, Article e54257. <a href=\"https://doi.org/10.1371/journal.pone.0054257\">https://doi.org/10.1371/journal.pone.0054257</a>","ieee":"K. Weiß, F. Hilkenmeier, and I. Scharlau, “Attention and the Speed of Information Processing: Posterior Entry for Unattended Stimuli Instead of Prior Entry for Attended Stimuli,” <i>PLoS ONE</i>, vol. 8, Art. no. e54257, 2013, doi: <a href=\"https://doi.org/10.1371/journal.pone.0054257\">10.1371/journal.pone.0054257</a>."}},{"external_id":{"pmid":["22629455"]},"citation":{"bibtex":"@article{Standl_Lattka_Stach_Koletzko_Bauer_von Berg_Berdel_Krämer_Schaaf_Röder_et al._2012, title={FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies.}, volume={7}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0037780\">10.1371/journal.pone.0037780</a>}, number={5}, journal={PLoS One}, author={Standl, M and Lattka, E and Stach, B and Koletzko, S and Bauer, CP and von Berg, A and Berdel, D and Krämer, U and Schaaf, B and Röder, S and et al.}, year={2012}, pages={e37780} }","ama":"Standl M, Lattka E, Stach B, et al. FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies. <i>PLoS One</i>. 2012;7(5):e37780. doi:<a href=\"https://doi.org/10.1371/journal.pone.0037780\">10.1371/journal.pone.0037780</a>","mla":"Standl, M., et al. “FADS1 FADS2 Gene Cluster, PUFA Intake and Blood Lipids in Children: Results from the GINIplus and LISAplus Studies.” <i>PLoS One</i>, vol. 7, no. 5, 2012, p. e37780, doi:<a href=\"https://doi.org/10.1371/journal.pone.0037780\">10.1371/journal.pone.0037780</a>.","chicago":"Standl, M, E Lattka, B Stach, S Koletzko, CP Bauer, A von Berg, D Berdel, et al. “FADS1 FADS2 Gene Cluster, PUFA Intake and Blood Lipids in Children: Results from the GINIplus and LISAplus Studies.” <i>PLoS One</i> 7, no. 5 (2012): e37780. <a href=\"https://doi.org/10.1371/journal.pone.0037780\">https://doi.org/10.1371/journal.pone.0037780</a>.","short":"M. Standl, E. Lattka, B. Stach, S. Koletzko, C. Bauer, A. von Berg, D. Berdel, U. Krämer, B. Schaaf, S. Röder, O. Herbarth, A. Buyken, T. Drogies, J. Thiery, B. Koletzko, J. Heinrich, Gini. Study Group, Lisa. Study Group, PLoS One 7 (2012) e37780.","ieee":"M. Standl <i>et al.</i>, “FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies.,” <i>PLoS One</i>, vol. 7, no. 5, p. e37780, 2012, doi: <a href=\"https://doi.org/10.1371/journal.pone.0037780\">10.1371/journal.pone.0037780</a>.","apa":"Standl, M., Lattka, E., Stach, B., Koletzko, S., Bauer, C., von Berg, A., Berdel, D., Krämer, U., Schaaf, B., Röder, S., Herbarth, O., Buyken, A., Drogies, T., Thiery, J., Koletzko, B., Heinrich, J., Study Group, Gini., &#38; Study Group, Lisa. (2012). FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies. <i>PLoS One</i>, <i>7</i>(5), e37780. <a href=\"https://doi.org/10.1371/journal.pone.0037780\">https://doi.org/10.1371/journal.pone.0037780</a>"},"user_id":"61597","volume":7,"page":"e37780","_id":"27986","status":"public","type":"journal_article","department":[{"_id":"17"},{"_id":"22"},{"_id":"571"}],"date_created":"2021-11-26T13:47:09Z","publication":"PLoS One","issue":"5","pmid":"1","doi":"10.1371/journal.pone.0037780","language":[{"iso":"eng"}],"date_updated":"2022-01-06T06:57:49Z","intvolume":"         7","year":"2012","title":"FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children: results from the GINIplus and LISAplus studies.","author":[{"full_name":"Standl, M","first_name":"M","last_name":"Standl"},{"last_name":"Lattka","first_name":"E","full_name":"Lattka, E"},{"last_name":"Stach","first_name":"B","full_name":"Stach, B"},{"last_name":"Koletzko","first_name":"S","full_name":"Koletzko, S"},{"first_name":"CP","last_name":"Bauer","full_name":"Bauer, CP"},{"full_name":"von Berg, A","first_name":"A","last_name":"von Berg"},{"full_name":"Berdel, D","last_name":"Berdel","first_name":"D"},{"last_name":"Krämer","first_name":"U","full_name":"Krämer, U"},{"first_name":"B","last_name":"Schaaf","full_name":"Schaaf, B"},{"full_name":"Röder, S","last_name":"Röder","first_name":"S"},{"first_name":"O","last_name":"Herbarth","full_name":"Herbarth, O"},{"full_name":"Buyken, Anette","first_name":"Anette","last_name":"Buyken","id":"65985"},{"full_name":"Drogies, T","first_name":"T","last_name":"Drogies"},{"first_name":"J","last_name":"Thiery","full_name":"Thiery, J"},{"last_name":"Koletzko","first_name":"B","full_name":"Koletzko, B"},{"full_name":"Heinrich, J","first_name":"J","last_name":"Heinrich"},{"full_name":"Study Group, GINIplus","first_name":"GINIplus","last_name":"Study Group"},{"full_name":"Study Group, LISAplus","last_name":"Study Group","first_name":"LISAplus"}],"publication_identifier":{"issn":["1932-6203"]}},{"date_created":"2024-06-30T13:53:18Z","department":[{"_id":"35"},{"_id":"22"}],"type":"journal_article","publication":"PLOS ONE","issue":"8","abstract":[{"text":"Background: Renal tubular epithelial cells of proximal and distal origin differ markedly in their physiological functions. Therefore, we hypothesized that they also differ in their capacity to undergo epithelial to mesenchymal alterations. Results: We used cultures of freshly isolated primary human tubular cells. To distinguish cells of different tubular origin we took advantage of the fact that human proximal epithelial cells uniquely express N-cadherin instead of E-cadherin as major cell-cell adhesion molecule. To provoke mesenchymal alteration we treated these cocultures with TGF-$\\beta$ for up to 6 days. Within this time period, the morphology of distal tubular cells was barely altered. In contrast to tubular cell lines, E-cadherin was not down-regulated by TGF-$\\beta$, even though TGF-$\\beta$ signal transduction was initiated as demonstrated by nuclear localization of Smad2/3. Analysis of transcription factors and miRNAs possibly involved in E-cadherin regulation revealed high levels of miRNAs of the miR200-family, which may contribute to the stability of E-cadherin expression in human distal tubular epithelial cells. By contrast, proximal tubular epithelial cells altered their phenotype when treated with TGF-$\\beta$. They became elongated and formed three-dimensional structures. Rho-kinases were identified as modulators of TGF-$\\beta$-induced morphological alterations. Non-specific inhibition of Rho-kinases resulted in stabilization of the epithelial phenotype, while partial effects were observed upon downregulation of Rho-kinase isoforms ROCK1 and ROCK2. The distinct reactivity of proximal and distal cells was retained when the cells were cultured as polarized cells. Conclusions: Interference with Rho-kinase signaling provides a target to counteract TGF-$\\beta$-mediated mesenchymal alterations of epithelial cells, particularly in proximal tubular epithelial cells. Furthermore, primary distal tubular cells differed from cell lines by their high phenotypic stability which included constant expression of E-cadherin. Our cell culture system of primary epithelial cells is thus suitable to understand and modulate cellular remodeling processes of distinct tubular cells relevant for human renal disease.","lang":"eng"}],"language":[{"iso":"eng"}],"doi":"10.1371/journal.pone.0043584","author":[{"first_name":"Christof","last_name":"Keller","full_name":"Keller, Christof"},{"last_name":"Kroening","first_name":"Sven","full_name":"Kroening, Sven"},{"first_name":"Jonathan","last_name":"Zuehlke","full_name":"Zuehlke, Jonathan"},{"last_name":"Kunath","first_name":"Frank","full_name":"Kunath, Frank"},{"id":"49428","full_name":"Krueger, Bettina","orcid":"0000-0001-5351-1785","last_name":"Krueger","first_name":"Bettina"},{"last_name":"Goppelt-Struebe","first_name":"Margarete","full_name":"Goppelt-Struebe, Margarete"}],"publication_identifier":{"issn":["1932-6203"]},"year":"2012","title":"Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells","intvolume":"         7","date_updated":"2024-06-30T13:53:30Z","citation":{"apa":"Keller, C., Kroening, S., Zuehlke, J., Kunath, F., Krueger, B., &#38; Goppelt-Struebe, M. (2012). Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells. <i>PLOS ONE</i>, <i>7</i>(8), e43584. <a href=\"https://doi.org/10.1371/journal.pone.0043584\">https://doi.org/10.1371/journal.pone.0043584</a>","ieee":"C. Keller, S. Kroening, J. Zuehlke, F. Kunath, B. Krueger, and M. Goppelt-Struebe, “Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells,” <i>PLOS ONE</i>, vol. 7, no. 8, p. e43584, 2012, doi: <a href=\"https://doi.org/10.1371/journal.pone.0043584\">10.1371/journal.pone.0043584</a>.","chicago":"Keller, Christof, Sven Kroening, Jonathan Zuehlke, Frank Kunath, Bettina Krueger, and Margarete Goppelt-Struebe. “Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells.” <i>PLOS ONE</i> 7, no. 8 (2012): e43584. <a href=\"https://doi.org/10.1371/journal.pone.0043584\">https://doi.org/10.1371/journal.pone.0043584</a>.","short":"C. Keller, S. Kroening, J. Zuehlke, F. Kunath, B. Krueger, M. Goppelt-Struebe, PLOS ONE 7 (2012) e43584.","mla":"Keller, Christof, et al. “Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells.” <i>PLOS ONE</i>, vol. 7, no. 8, Public Library of Science, 2012, p. e43584, doi:<a href=\"https://doi.org/10.1371/journal.pone.0043584\">10.1371/journal.pone.0043584</a>.","ama":"Keller C, Kroening S, Zuehlke J, Kunath F, Krueger B, Goppelt-Struebe M. Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells. <i>PLOS ONE</i>. 2012;7(8):e43584. doi:<a href=\"https://doi.org/10.1371/journal.pone.0043584\">10.1371/journal.pone.0043584</a>","bibtex":"@article{Keller_Kroening_Zuehlke_Kunath_Krueger_Goppelt-Struebe_2012, title={Distinct Mesenchymal Alterations in N-Cadherin and E-Cadherin Positive Primary Renal Epithelial Cells}, volume={7}, DOI={<a href=\"https://doi.org/10.1371/journal.pone.0043584\">10.1371/journal.pone.0043584</a>}, number={8}, journal={PLOS ONE}, publisher={Public Library of Science}, author={Keller, Christof and Kroening, Sven and Zuehlke, Jonathan and Kunath, Frank and Krueger, Bettina and Goppelt-Struebe, Margarete}, year={2012}, pages={e43584} }"},"publisher":"Public Library of Science","_id":"54939","page":"e43584","volume":7,"user_id":"49428","status":"public"}]