[{"language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"<jats:title>Abstract</jats:title><jats:p>Cellular stress and ageing involve an increase in crowding and aggregation of amylogenic proteins. We here investigate if crowding is the intrinsic cause of aggregation and utilise a previously established non-protein aggregation sensor, namely pseudoisocyanine chloride (PIC). PIC shows fibrillization in cells into a highly fluorescent J-aggregated state and is sensitive to crowding. Surprisingly, cell stress conditions stabilise the monomeric rather than the aggregated state of PIC both in the cytoplasm and in stress granules. Regarding the different physiochemical changes of the cytoplasm occurring upon cell stress, involving volume reduction, phase separation and solidification, the intrinsic crowding effect is not the key factor to drive associated self-assembly processes.</jats:p>"}],"publication":"Communications Chemistry","title":"Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor","date_created":"2025-11-19T09:51:55Z","publisher":"Springer Science and Business Media LLC","year":"2024","issue":"1","quality_controlled":"1","article_number":"230","department":[{"_id":"314"}],"user_id":"237","_id":"62255","status":"public","type":"journal_article","doi":"10.1038/s42004-024-01315-y","volume":7,"author":[{"last_name":"Pollak","full_name":"Pollak, Roland","first_name":"Roland"},{"first_name":"Leon","full_name":"Koch, Leon","last_name":"Koch"},{"full_name":"König, Benedikt","last_name":"König","first_name":"Benedikt"},{"first_name":"Sara S.","full_name":"Ribeiro, Sara S.","last_name":"Ribeiro"},{"last_name":"Samanta","full_name":"Samanta, Nirnay","first_name":"Nirnay"},{"id":"237","full_name":"Huber, Klaus","last_name":"Huber","first_name":"Klaus"},{"first_name":"Simon","last_name":"Ebbinghaus","full_name":"Ebbinghaus, Simon"}],"date_updated":"2025-11-19T10:06:01Z","intvolume":"         7","citation":{"apa":"Pollak, R., Koch, L., König, B., Ribeiro, S. S., Samanta, N., Huber, K., &#38; Ebbinghaus, S. (2024). Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor. <i>Communications Chemistry</i>, <i>7</i>(1), Article 230. <a href=\"https://doi.org/10.1038/s42004-024-01315-y\">https://doi.org/10.1038/s42004-024-01315-y</a>","mla":"Pollak, Roland, et al. “Cell Stress and Phase Separation Stabilize the Monomeric State of Pseudoisocyanine Chloride Employed as a Self-Assembly Crowding Sensor.” <i>Communications Chemistry</i>, vol. 7, no. 1, 230, Springer Science and Business Media LLC, 2024, doi:<a href=\"https://doi.org/10.1038/s42004-024-01315-y\">10.1038/s42004-024-01315-y</a>.","short":"R. Pollak, L. Koch, B. König, S.S. Ribeiro, N. Samanta, K. Huber, S. Ebbinghaus, Communications Chemistry 7 (2024).","bibtex":"@article{Pollak_Koch_König_Ribeiro_Samanta_Huber_Ebbinghaus_2024, title={Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor}, volume={7}, DOI={<a href=\"https://doi.org/10.1038/s42004-024-01315-y\">10.1038/s42004-024-01315-y</a>}, number={1230}, journal={Communications Chemistry}, publisher={Springer Science and Business Media LLC}, author={Pollak, Roland and Koch, Leon and König, Benedikt and Ribeiro, Sara S. and Samanta, Nirnay and Huber, Klaus and Ebbinghaus, Simon}, year={2024} }","ama":"Pollak R, Koch L, König B, et al. Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor. <i>Communications Chemistry</i>. 2024;7(1). doi:<a href=\"https://doi.org/10.1038/s42004-024-01315-y\">10.1038/s42004-024-01315-y</a>","ieee":"R. Pollak <i>et al.</i>, “Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding sensor,” <i>Communications Chemistry</i>, vol. 7, no. 1, Art. no. 230, 2024, doi: <a href=\"https://doi.org/10.1038/s42004-024-01315-y\">10.1038/s42004-024-01315-y</a>.","chicago":"Pollak, Roland, Leon Koch, Benedikt König, Sara S. Ribeiro, Nirnay Samanta, Klaus Huber, and Simon Ebbinghaus. “Cell Stress and Phase Separation Stabilize the Monomeric State of Pseudoisocyanine Chloride Employed as a Self-Assembly Crowding Sensor.” <i>Communications Chemistry</i> 7, no. 1 (2024). <a href=\"https://doi.org/10.1038/s42004-024-01315-y\">https://doi.org/10.1038/s42004-024-01315-y</a>."},"publication_identifier":{"issn":["2399-3669"]},"publication_status":"published"},{"publication_status":"published","publication_identifier":{"issn":["2399-3669"]},"issue":"1","year":"2019","citation":{"chicago":"Ojha, Deepak, Naveen Kumar Kaliannan, and Thomas D. Kühne. “Time-Dependent Vibrational Sum-Frequency Generation Spectroscopy of the Air-Water Interface.” <i>Communications Chemistry</i> 2, no. 1 (2019): 116. <a href=\"https://doi.org/10.1038/s42004-019-0220-6\">https://doi.org/10.1038/s42004-019-0220-6</a>.","ieee":"D. Ojha, N. K. Kaliannan, and T. D. Kühne, “Time-dependent vibrational sum-frequency generation spectroscopy of the air-water interface,” <i>Communications Chemistry</i>, vol. 2, no. 1, p. 116, 2019.","ama":"Ojha D, Kaliannan NK, Kühne TD. Time-dependent vibrational sum-frequency generation spectroscopy of the air-water interface. <i>Communications Chemistry</i>. 2019;2(1):116. doi:<a href=\"https://doi.org/10.1038/s42004-019-0220-6\">10.1038/s42004-019-0220-6</a>","apa":"Ojha, D., Kaliannan, N. K., &#38; Kühne, T. D. (2019). Time-dependent vibrational sum-frequency generation spectroscopy of the air-water interface. <i>Communications Chemistry</i>, <i>2</i>(1), 116. <a href=\"https://doi.org/10.1038/s42004-019-0220-6\">https://doi.org/10.1038/s42004-019-0220-6</a>","bibtex":"@article{Ojha_Kaliannan_Kühne_2019, title={Time-dependent vibrational sum-frequency generation spectroscopy of the air-water interface}, volume={2}, DOI={<a href=\"https://doi.org/10.1038/s42004-019-0220-6\">10.1038/s42004-019-0220-6</a>}, number={1}, journal={Communications Chemistry}, author={Ojha, Deepak and Kaliannan, Naveen Kumar and Kühne, Thomas D.}, year={2019}, pages={116} }","short":"D. Ojha, N.K. Kaliannan, T.D. Kühne, Communications Chemistry 2 (2019) 116.","mla":"Ojha, Deepak, et al. “Time-Dependent Vibrational Sum-Frequency Generation Spectroscopy of the Air-Water Interface.” <i>Communications Chemistry</i>, vol. 2, no. 1, 2019, p. 116, doi:<a href=\"https://doi.org/10.1038/s42004-019-0220-6\">10.1038/s42004-019-0220-6</a>."},"page":"116","intvolume":"         2","date_updated":"2022-01-06T06:52:32Z","author":[{"first_name":"Deepak","full_name":"Ojha, Deepak","last_name":"Ojha"},{"first_name":"Naveen Kumar","last_name":"Kaliannan","full_name":"Kaliannan, Naveen Kumar"},{"first_name":"Thomas D.","last_name":"Kühne","full_name":"Kühne, Thomas D."}],"date_created":"2020-01-30T13:06:44Z","volume":2,"title":"Time-dependent vibrational sum-frequency generation spectroscopy of the air-water interface","doi":"10.1038/s42004-019-0220-6","type":"journal_article","publication":"Communications Chemistry","status":"public","project":[{"name":"Computing Resources Provided by the Paderborn Center for Parallel Computing","_id":"52"}],"_id":"15724","user_id":"71692","language":[{"iso":"eng"}]}]