---
_id: '62255'
abstract:
- lang: eng
  text: <jats:title>Abstract</jats:title><jats:p>Cellular stress and ageing involve
    an increase in crowding and aggregation of amylogenic proteins. We here investigate
    if crowding is the intrinsic cause of aggregation and utilise a previously established
    non-protein aggregation sensor, namely pseudoisocyanine chloride (PIC). PIC shows
    fibrillization in cells into a highly fluorescent J-aggregated state and is sensitive
    to crowding. Surprisingly, cell stress conditions stabilise the monomeric rather
    than the aggregated state of PIC both in the cytoplasm and in stress granules.
    Regarding the different physiochemical changes of the cytoplasm occurring upon
    cell stress, involving volume reduction, phase separation and solidification,
    the intrinsic crowding effect is not the key factor to drive associated self-assembly
    processes.</jats:p>
article_number: '230'
author:
- first_name: Roland
  full_name: Pollak, Roland
  last_name: Pollak
- first_name: Leon
  full_name: Koch, Leon
  last_name: Koch
- first_name: Benedikt
  full_name: König, Benedikt
  last_name: König
- first_name: Sara S.
  full_name: Ribeiro, Sara S.
  last_name: Ribeiro
- first_name: Nirnay
  full_name: Samanta, Nirnay
  last_name: Samanta
- first_name: Klaus
  full_name: Huber, Klaus
  id: '237'
  last_name: Huber
- first_name: Simon
  full_name: Ebbinghaus, Simon
  last_name: Ebbinghaus
citation:
  ama: Pollak R, Koch L, König B, et al. Cell stress and phase separation stabilize
    the monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding
    sensor. <i>Communications Chemistry</i>. 2024;7(1). doi:<a href="https://doi.org/10.1038/s42004-024-01315-y">10.1038/s42004-024-01315-y</a>
  apa: Pollak, R., Koch, L., König, B., Ribeiro, S. S., Samanta, N., Huber, K., &#38;
    Ebbinghaus, S. (2024). Cell stress and phase separation stabilize the monomeric
    state of pseudoisocyanine chloride employed as a self-assembly crowding sensor.
    <i>Communications Chemistry</i>, <i>7</i>(1), Article 230. <a href="https://doi.org/10.1038/s42004-024-01315-y">https://doi.org/10.1038/s42004-024-01315-y</a>
  bibtex: '@article{Pollak_Koch_König_Ribeiro_Samanta_Huber_Ebbinghaus_2024, title={Cell
    stress and phase separation stabilize the monomeric state of pseudoisocyanine
    chloride employed as a self-assembly crowding sensor}, volume={7}, DOI={<a href="https://doi.org/10.1038/s42004-024-01315-y">10.1038/s42004-024-01315-y</a>},
    number={1230}, journal={Communications Chemistry}, publisher={Springer Science
    and Business Media LLC}, author={Pollak, Roland and Koch, Leon and König, Benedikt
    and Ribeiro, Sara S. and Samanta, Nirnay and Huber, Klaus and Ebbinghaus, Simon},
    year={2024} }'
  chicago: Pollak, Roland, Leon Koch, Benedikt König, Sara S. Ribeiro, Nirnay Samanta,
    Klaus Huber, and Simon Ebbinghaus. “Cell Stress and Phase Separation Stabilize
    the Monomeric State of Pseudoisocyanine Chloride Employed as a Self-Assembly Crowding
    Sensor.” <i>Communications Chemistry</i> 7, no. 1 (2024). <a href="https://doi.org/10.1038/s42004-024-01315-y">https://doi.org/10.1038/s42004-024-01315-y</a>.
  ieee: 'R. Pollak <i>et al.</i>, “Cell stress and phase separation stabilize the
    monomeric state of pseudoisocyanine chloride employed as a self-assembly crowding
    sensor,” <i>Communications Chemistry</i>, vol. 7, no. 1, Art. no. 230, 2024, doi:
    <a href="https://doi.org/10.1038/s42004-024-01315-y">10.1038/s42004-024-01315-y</a>.'
  mla: Pollak, Roland, et al. “Cell Stress and Phase Separation Stabilize the Monomeric
    State of Pseudoisocyanine Chloride Employed as a Self-Assembly Crowding Sensor.”
    <i>Communications Chemistry</i>, vol. 7, no. 1, 230, Springer Science and Business
    Media LLC, 2024, doi:<a href="https://doi.org/10.1038/s42004-024-01315-y">10.1038/s42004-024-01315-y</a>.
  short: R. Pollak, L. Koch, B. König, S.S. Ribeiro, N. Samanta, K. Huber, S. Ebbinghaus,
    Communications Chemistry 7 (2024).
date_created: 2025-11-19T09:51:55Z
date_updated: 2025-11-19T10:06:01Z
department:
- _id: '314'
doi: 10.1038/s42004-024-01315-y
intvolume: '         7'
issue: '1'
language:
- iso: eng
publication: Communications Chemistry
publication_identifier:
  issn:
  - 2399-3669
publication_status: published
publisher: Springer Science and Business Media LLC
quality_controlled: '1'
status: public
title: Cell stress and phase separation stabilize the monomeric state of pseudoisocyanine
  chloride employed as a self-assembly crowding sensor
type: journal_article
user_id: '237'
volume: 7
year: '2024'
...
---
_id: '15724'
author:
- first_name: Deepak
  full_name: Ojha, Deepak
  last_name: Ojha
- first_name: Naveen Kumar
  full_name: Kaliannan, Naveen Kumar
  last_name: Kaliannan
- first_name: Thomas D.
  full_name: Kühne, Thomas D.
  last_name: Kühne
citation:
  ama: Ojha D, Kaliannan NK, Kühne TD. Time-dependent vibrational sum-frequency generation
    spectroscopy of the air-water interface. <i>Communications Chemistry</i>. 2019;2(1):116.
    doi:<a href="https://doi.org/10.1038/s42004-019-0220-6">10.1038/s42004-019-0220-6</a>
  apa: Ojha, D., Kaliannan, N. K., &#38; Kühne, T. D. (2019). Time-dependent vibrational
    sum-frequency generation spectroscopy of the air-water interface. <i>Communications
    Chemistry</i>, <i>2</i>(1), 116. <a href="https://doi.org/10.1038/s42004-019-0220-6">https://doi.org/10.1038/s42004-019-0220-6</a>
  bibtex: '@article{Ojha_Kaliannan_Kühne_2019, title={Time-dependent vibrational sum-frequency
    generation spectroscopy of the air-water interface}, volume={2}, DOI={<a href="https://doi.org/10.1038/s42004-019-0220-6">10.1038/s42004-019-0220-6</a>},
    number={1}, journal={Communications Chemistry}, author={Ojha, Deepak and Kaliannan,
    Naveen Kumar and Kühne, Thomas D.}, year={2019}, pages={116} }'
  chicago: 'Ojha, Deepak, Naveen Kumar Kaliannan, and Thomas D. Kühne. “Time-Dependent
    Vibrational Sum-Frequency Generation Spectroscopy of the Air-Water Interface.”
    <i>Communications Chemistry</i> 2, no. 1 (2019): 116. <a href="https://doi.org/10.1038/s42004-019-0220-6">https://doi.org/10.1038/s42004-019-0220-6</a>.'
  ieee: D. Ojha, N. K. Kaliannan, and T. D. Kühne, “Time-dependent vibrational sum-frequency
    generation spectroscopy of the air-water interface,” <i>Communications Chemistry</i>,
    vol. 2, no. 1, p. 116, 2019.
  mla: Ojha, Deepak, et al. “Time-Dependent Vibrational Sum-Frequency Generation Spectroscopy
    of the Air-Water Interface.” <i>Communications Chemistry</i>, vol. 2, no. 1, 2019,
    p. 116, doi:<a href="https://doi.org/10.1038/s42004-019-0220-6">10.1038/s42004-019-0220-6</a>.
  short: D. Ojha, N.K. Kaliannan, T.D. Kühne, Communications Chemistry 2 (2019) 116.
date_created: 2020-01-30T13:06:44Z
date_updated: 2022-01-06T06:52:32Z
doi: 10.1038/s42004-019-0220-6
intvolume: '         2'
issue: '1'
language:
- iso: eng
page: '116'
project:
- _id: '52'
  name: Computing Resources Provided by the Paderborn Center for Parallel Computing
publication: Communications Chemistry
publication_identifier:
  issn:
  - 2399-3669
publication_status: published
status: public
title: Time-dependent vibrational sum-frequency generation spectroscopy of the air-water
  interface
type: journal_article
user_id: '71692'
volume: 2
year: '2019'
...