@article{65490,
  abstract     = {{<jats:p>In recent years, nanostructures assembled by DNA have found promising applications in optics, medicine, and sensing. DNA origami in particular provides unique self‐assembly properties, not only enabling a vast variety of functionalization schemes but also presenting a promising route to fabricate large‐scale, bottom‐up nanostructured arrays. This approach has comparable precision to electron beam lithography but avoids slow and expensive patterning steps. However, self‐assembly of lattices with high order and well‐defined periodicity requires careful tuning of the deposition parameters and interactions involved, which has been done mostly on mica so far. As mica is not compatible with standard microfabrication processes, we investigate here the assembly of DNA origami lattices on the most general microfabrication material, that is, silicon wafers, which has turned out to be rather challenging. We study how the forming of polycrystalline 2D‐fishnet‐type lattices is influenced by different incubation conditions and strengths of the origami–origami and origami‐surface interactions, with the aim to create large‐scale single‐crystalline lattices. The lattices are characterized by atomic force microscopy and analyzed for precision of formation, achievable domain size, and surface coverage of well‐formed lattices. Thanks to the silicon substrate, these DNA origami lattices can be further combined with traditional microfabrication processes to turn them, for example, into metamaterials with novel optical properties.</jats:p>}},
  author       = {{Järvinen, Heini and Parikka, Johannes M. and Rajapaksha, R. P. Thiwangi N. and Keller, Adrian Clemens and Toppari, J. Jussi}},
  issn         = {{2688-4062}},
  journal      = {{Small Structures}},
  number       = {{4}},
  publisher    = {{Wiley}},
  title        = {{{Towards Single‐Crystalline DNA Origami Lattices on Silicon Wafers for Bottom‐Up Nanofabrication}}},
  doi          = {{10.1002/sstr.202500813}},
  volume       = {{7}},
  year         = {{2026}},
}

@article{60973,
  abstract     = {{<jats:p>The specific binding of DNA origami nanostructures (DONs) to bacteria is an important prerequisite for their application in pathogen targeting and antimicrobial drug delivery. So far, targeting bacteria with DONs has been achieved exclusively via aptamers, which suffer from drawbacks such as sensitivity toward environmental conditions and reduced binding after immobilization or conjugation. Here, an alternative approach is presented based on the modification of DONs with the cell wall‐binding glycopeptide antibiotic vancomycin. Using strain‐promoted azide‐alkyne cycloaddition, azide‐modified vancomycin is conjugated to selected staple strands and subsequently incorporated into 2D DON triangles. The resulting constructs show specific binding to the Gram‐positive species <jats:italic>Bacillus subtilis</jats:italic> (<jats:italic>B. subtilis</jats:italic>) and <jats:italic>Staphylococcus capitis</jats:italic> (<jats:italic>S. capitis</jats:italic>), and remarkably, to Gram‐negative <jats:italic>Escherichia coli</jats:italic> (<jats:italic>E. coli</jats:italic>), but no antimicrobial activity at vancomycin concentrations up to at least 2.91 μM. For <jats:italic>B. subtilis</jats:italic> and <jats:italic>E. coli</jats:italic>, DONs with vancomycin modifications on both sides exhibit better binding than DONs modified on only one side. However, both variants bind equally well to <jats:italic>S. capitis</jats:italic>. These results demonstrate the great potential of small molecule drug compounds for the robust, broad‐spectrum targeting of bacteria with DONs. Targeting a ubiquitous cell wall component of most pathogenic bacteria, vancomycin‐modified DONs have many potential applications in the prevention and treatment of nosocomial infections.</jats:p>}},
  author       = {{Coşkuner Leineweber, Özge and Pothineni, Bhanu K. and Schumann, Nils and Hofmann, Ulrike and Möser, Christin and Smith, David M. and Grundmeier, Guido and Zhang, Yixin and Keller, Adrian}},
  issn         = {{2688-4062}},
  journal      = {{Small Structures}},
  publisher    = {{Wiley}},
  title        = {{{Vancomycin‐Modified DNA Origami Nanostructures for Targeting Bacterial Pathogens}}},
  doi          = {{10.1002/sstr.202500246}},
  year         = {{2025}},
}

@article{55310,
  abstract     = {{<jats:p>DNA origami nanostructures are promising carries for drug delivery applications. However, their limited stability under relevant conditions often presents a challenge. Herein, the structural stability of DNA origami nanostructures is investigated in a setting compatible with their application in photodynamic therapy (PDT). To this end, DNA origami triangles and six‐helix bundles (6HBs) are loaded with the clinically tested photosensitizer methylene blue, which upon irradiation with red light generates reactive oxygen species (ROS) that attack the DNA origami nanostructures. ROS‐induced structural damage is observed to depend on the ionic composition of the surrounding medium and becomes more severe at low ionic strength. Mg<jats:sup>2+</jats:sup> ions can efficiently protect the DNA origami nanostructures from ROS‐induced damage and may even heal some of the damage obtained under Mg<jats:sup>2+</jats:sup>‐free conditions when added after irradiation. Finally, the employed DNA origami 6HBs are more resistant toward ROS‐induced structural damage than the triangles, which is attributed to their markedly different mechanical properties. These results thus provide some fundamental insights into the stabilizing role of DNA origami superstructure that may guide the selection or design of DNA origami nanocarriers with optimized stability for their application in PDT.</jats:p>}},
  author       = {{Rabbe, Lukas and Garcia‐Diosa, Jaime Andres and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{2688-4062}},
  journal      = {{Small Structures}},
  publisher    = {{Wiley}},
  title        = {{{Ion‐Dependent Stability of DNA Origami Nanostructures in the Presence of Photo‐Generated Reactive Oxygen Species}}},
  doi          = {{10.1002/sstr.202400094}},
  year         = {{2024}},
}

@article{22684,
  author       = {{Huang, Jingyuan and Suma, Antonio and Cui, Meiying and Grundmeier, Guido and Carnevale, Vincenzo and Zhang, Yixin and Kielar, Charlotte and Keller, Adrian}},
  issn         = {{2688-4062}},
  journal      = {{Small Structures}},
  pages        = {{2000038}},
  title        = {{{Arranging Small Molecules with Subnanometer Precision on DNA Origami Substrates for the Single‐Molecule Investigation of Protein–Ligand Interactions}}},
  doi          = {{10.1002/sstr.202000038}},
  volume       = {{1}},
  year         = {{2020}},
}