---
_id: '59847'
abstract:
- lang: eng
  text: "<jats:title>Abstract</jats:title>\r\n          <jats:p>The surface-assisted
    assembly of DNA origami lattices is a potent method for creating molecular lithography
    masks. Lattice quality and assembly kinetics are controlled by various environmental
    parameters, including the employed surface, the assembly temperature, and the
    ionic composition of the buffer, with optimized parameter combinations resulting
    in highly ordered lattices that can span surface areas of several cm<jats:sup>2</jats:sup>.
    Established assembly protocols, however, employ assembly times ranging from hours
    to days. Here, the assembly of highly ordered hexagonal DNA origami lattices at
    mica surfaces is observed within few minutes using high-speed atomic force microscopy
    (HS-AFM). A moderate increase in the DNA origami concentration enables this rapid
    assembly. While forming a regular lattice takes 10 min at a DNA origami concentration
    of 4 nM, this time is shortened to about 2 min at a concentration of 6 nM. Increasing
    the DNA origami concentration any further does not result in shorter assembly
    times, presumably because DNA origami arrival at the mica surface is diffusion-limited.
    Over short length scales up to 1 µm, lattice order is independent of the DNA origami
    concentration. However, at larger length scales of a few microns, a DNA origami
    concentration of 10 nM yields slightly better order than lower and higher concentrations.
    Therefore, 10 nM can be considered the optimum concentration for the rapid assembly
    of highly ordered DNA origami lattices. These results thus represent an important
    step toward the industrial-scale application of DNA origami-based lithography
    masks.</jats:p>"
article_number: '77'
author:
- first_name: Bhanu Kiran
  full_name: Pothineni, Bhanu Kiran
  last_name: Pothineni
- first_name: Jörg
  full_name: Barner, Jörg
  last_name: Barner
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
- first_name: David
  full_name: Contreras, David
  last_name: Contreras
- first_name: Mario
  full_name: Castro, Mario
  last_name: Castro
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
citation:
  ama: Pothineni BK, Barner J, Grundmeier G, Contreras D, Castro M, Keller A. Rapid
    assembly of highly ordered DNA origami lattices at mica surfaces. <i>Discover
    Nano</i>. 2025;20(1). doi:<a href="https://doi.org/10.1186/s11671-025-04254-2">10.1186/s11671-025-04254-2</a>
  apa: Pothineni, B. K., Barner, J., Grundmeier, G., Contreras, D., Castro, M., &#38;
    Keller, A. (2025). Rapid assembly of highly ordered DNA origami lattices at mica
    surfaces. <i>Discover Nano</i>, <i>20</i>(1), Article 77. <a href="https://doi.org/10.1186/s11671-025-04254-2">https://doi.org/10.1186/s11671-025-04254-2</a>
  bibtex: '@article{Pothineni_Barner_Grundmeier_Contreras_Castro_Keller_2025, title={Rapid
    assembly of highly ordered DNA origami lattices at mica surfaces}, volume={20},
    DOI={<a href="https://doi.org/10.1186/s11671-025-04254-2">10.1186/s11671-025-04254-2</a>},
    number={177}, journal={Discover Nano}, publisher={Springer Science and Business
    Media LLC}, author={Pothineni, Bhanu Kiran and Barner, Jörg and Grundmeier, Guido
    and Contreras, David and Castro, Mario and Keller, Adrian}, year={2025} }'
  chicago: Pothineni, Bhanu Kiran, Jörg Barner, Guido Grundmeier, David Contreras,
    Mario Castro, and Adrian Keller. “Rapid Assembly of Highly Ordered DNA Origami
    Lattices at Mica Surfaces.” <i>Discover Nano</i> 20, no. 1 (2025). <a href="https://doi.org/10.1186/s11671-025-04254-2">https://doi.org/10.1186/s11671-025-04254-2</a>.
  ieee: 'B. K. Pothineni, J. Barner, G. Grundmeier, D. Contreras, M. Castro, and A.
    Keller, “Rapid assembly of highly ordered DNA origami lattices at mica surfaces,”
    <i>Discover Nano</i>, vol. 20, no. 1, Art. no. 77, 2025, doi: <a href="https://doi.org/10.1186/s11671-025-04254-2">10.1186/s11671-025-04254-2</a>.'
  mla: Pothineni, Bhanu Kiran, et al. “Rapid Assembly of Highly Ordered DNA Origami
    Lattices at Mica Surfaces.” <i>Discover Nano</i>, vol. 20, no. 1, 77, Springer
    Science and Business Media LLC, 2025, doi:<a href="https://doi.org/10.1186/s11671-025-04254-2">10.1186/s11671-025-04254-2</a>.
  short: B.K. Pothineni, J. Barner, G. Grundmeier, D. Contreras, M. Castro, A. Keller,
    Discover Nano 20 (2025).
date_created: 2025-05-08T07:17:29Z
date_updated: 2025-05-08T07:17:54Z
department:
- _id: '302'
doi: 10.1186/s11671-025-04254-2
intvolume: '        20'
issue: '1'
language:
- iso: eng
publication: Discover Nano
publication_identifier:
  issn:
  - 2731-9229
publication_status: published
publisher: Springer Science and Business Media LLC
status: public
title: Rapid assembly of highly ordered DNA origami lattices at mica surfaces
type: journal_article
user_id: '48864'
volume: 20
year: '2025'
...