[{"citation":{"ama":"Duderija B, González-Orive A, Ebbert C, Neßlinger V, Keller A, Grundmeier G. Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy. <i>Molecules</i>. 2023;28(13). doi:<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>","chicago":"Duderija, Belma, Alejandro González-Orive, Christoph Ebbert, Vanessa Neßlinger, Adrian Keller, and Guido Grundmeier. “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i> 28, no. 13 (2023). <a href=\"https://doi.org/10.3390/molecules28135109\">https://doi.org/10.3390/molecules28135109</a>.","ieee":"B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, and G. Grundmeier, “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy,” <i>Molecules</i>, vol. 28, no. 13, Art. no. 5109, 2023, doi: <a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>.","bibtex":"@article{Duderija_González-Orive_Ebbert_Neßlinger_Keller_Grundmeier_2023, title={Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy}, volume={28}, DOI={<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>}, number={135109}, journal={Molecules}, publisher={MDPI AG}, author={Duderija, Belma and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and Keller, Adrian and Grundmeier, Guido}, year={2023} }","short":"B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, G. Grundmeier, Molecules 28 (2023).","mla":"Duderija, Belma, et al. “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i>, vol. 28, no. 13, 5109, MDPI AG, 2023, doi:<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>.","apa":"Duderija, B., González-Orive, A., Ebbert, C., Neßlinger, V., Keller, A., &#38; Grundmeier, G. (2023). Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy. <i>Molecules</i>, <i>28</i>(13), Article 5109. <a href=\"https://doi.org/10.3390/molecules28135109\">https://doi.org/10.3390/molecules28135109</a>"},"intvolume":"        28","year":"2023","issue":"13","publication_status":"published","publication_identifier":{"issn":["1420-3049"]},"doi":"10.3390/molecules28135109","title":"Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy","date_created":"2023-07-12T07:55:40Z","author":[{"first_name":"Belma","last_name":"Duderija","full_name":"Duderija, Belma"},{"full_name":"González-Orive, Alejandro","last_name":"González-Orive","first_name":"Alejandro"},{"first_name":"Christoph","full_name":"Ebbert, Christoph","last_name":"Ebbert"},{"full_name":"Neßlinger, Vanessa","last_name":"Neßlinger","first_name":"Vanessa"},{"first_name":"Adrian","full_name":"Keller, Adrian","last_name":"Keller"},{"full_name":"Grundmeier, Guido","last_name":"Grundmeier","first_name":"Guido"}],"volume":28,"publisher":"MDPI AG","date_updated":"2024-02-06T12:33:55Z","status":"public","abstract":[{"text":"<jats:p>This article presents the potential-dependent adsorption of two proteins, bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and 37 °C. The adsorption process was studied on an electropolished alloy under cathodic and anodic overpotentials, compared to the open circuit potential (OCP). To analyze the adsorption process, various complementary interface analytical techniques were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy), and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization experiments were conducted within a potential range where charging of the electric double layer dominates, and Faradaic currents can be disregarded. The findings highlight the significant influence of the interfacial charge distribution on the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical analysis of the protein layers formed under applied overpotentials demonstrated improved corrosion protection properties. These studies provide valuable insights into protein adsorption on titanium alloys under physiological conditions, characterized by varying potentials of the passive alloy.</jats:p>","lang":"eng"}],"type":"journal_article","publication":"Molecules","language":[{"iso":"eng"}],"article_number":"5109","keyword":["Chemistry (miscellaneous)","Analytical Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Molecular Medicine","Drug Discovery","Pharmaceutical Science"],"user_id":"54863","department":[{"_id":"321"},{"_id":"302"}],"_id":"46023"},{"_id":"45828","department":[{"_id":"302"}],"user_id":"48864","status":"public","type":"journal_article","doi":"10.3390/molecules28135109","date_updated":"2023-07-03T08:07:55Z","volume":28,"author":[{"last_name":"Duderija","id":"54863","full_name":"Duderija, Belma","first_name":"Belma"},{"full_name":"González-Orive, Alejandro","last_name":"González-Orive","first_name":"Alejandro"},{"first_name":"Christoph","full_name":"Ebbert, Christoph","id":"7266","last_name":"Ebbert"},{"first_name":"Vanessa","full_name":"Neßlinger, Vanessa","last_name":"Neßlinger"},{"id":"48864","full_name":"Keller, Adrian","orcid":"0000-0001-7139-3110","last_name":"Keller","first_name":"Adrian"},{"full_name":"Grundmeier, Guido","id":"194","last_name":"Grundmeier","first_name":"Guido"}],"intvolume":"        28","page":"5109","citation":{"apa":"Duderija, B., González-Orive, A., Ebbert, C., Neßlinger, V., Keller, A., &#38; Grundmeier, G. (2023). Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy. <i>Molecules</i>, <i>28</i>(13), 5109. <a href=\"https://doi.org/10.3390/molecules28135109\">https://doi.org/10.3390/molecules28135109</a>","mla":"Duderija, Belma, et al. “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i>, vol. 28, no. 13, MDPI AG, 2023, p. 5109, doi:<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>.","bibtex":"@article{Duderija_González-Orive_Ebbert_Neßlinger_Keller_Grundmeier_2023, title={Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy}, volume={28}, DOI={<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>}, number={13}, journal={Molecules}, publisher={MDPI AG}, author={Duderija, Belma and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and Keller, Adrian and Grundmeier, Guido}, year={2023}, pages={5109} }","short":"B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, G. Grundmeier, Molecules 28 (2023) 5109.","chicago":"Duderija, Belma, Alejandro González-Orive, Christoph Ebbert, Vanessa Neßlinger, Adrian Keller, and Guido Grundmeier. “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i> 28, no. 13 (2023): 5109. <a href=\"https://doi.org/10.3390/molecules28135109\">https://doi.org/10.3390/molecules28135109</a>.","ieee":"B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, and G. Grundmeier, “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy,” <i>Molecules</i>, vol. 28, no. 13, p. 5109, 2023, doi: <a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>.","ama":"Duderija B, González-Orive A, Ebbert C, Neßlinger V, Keller A, Grundmeier G. Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy. <i>Molecules</i>. 2023;28(13):5109. doi:<a href=\"https://doi.org/10.3390/molecules28135109\">10.3390/molecules28135109</a>"},"publication_identifier":{"issn":["1420-3049"]},"publication_status":"published","keyword":["Chemistry (miscellaneous)","Analytical Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Molecular Medicine","Drug Discovery","Pharmaceutical Science"],"language":[{"iso":"eng"}],"abstract":[{"text":"<jats:p>This article presents the potential-dependent adsorption of two proteins, bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and 37 °C. The adsorption process was studied on an electropolished alloy under cathodic and anodic overpotentials, compared to the open circuit potential (OCP). To analyze the adsorption process, various complementary interface analytical techniques were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy), and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization experiments were conducted within a potential range where charging of the electric double layer dominates, and Faradaic currents can be disregarded. The findings highlight the significant influence of the interfacial charge distribution on the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical analysis of the protein layers formed under applied overpotentials demonstrated improved corrosion protection properties. These studies provide valuable insights into protein adsorption on titanium alloys under physiological conditions, characterized by varying potentials of the passive alloy.</jats:p>","lang":"eng"}],"publication":"Molecules","title":"Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy","publisher":"MDPI AG","date_created":"2023-07-03T08:06:28Z","year":"2023","issue":"13"},{"issue":"16","year":"2023","date_created":"2023-08-16T10:51:48Z","publisher":"MDPI AG","title":"Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy","publication":"Molecules","abstract":[{"text":"<jats:p>Multiprotein adsorption from complex body fluids represents a highly important and complicated phenomenon in medicine. In this work, multiprotein adsorption from diluted human serum at gold and oxidized iron surfaces is investigated at different serum concentrations and pH values. Adsorption-induced changes in surface topography and the total amount of adsorbed proteins are quantified by atomic force microscopy (AFM) and polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS), respectively. For both surfaces, stronger protein adsorption is observed at pH 6 compared to pH 7 and pH 8. PM-IRRAS furthermore provides some qualitative insights into the pH-dependent alterations in the composition of the adsorbed multiprotein films. Changes in the amide II/amide I band area ratio and in particular side-chain IR absorption suggest that the increased adsorption at pH 6 is accompanied by a change in protein film composition. Presumably, this is mostly driven by the adsorption of human serum albumin, which at pH 6 adsorbs more readily and thereby replaces other proteins with lower surface affinities in the resulting multiprotein film.</jats:p>","lang":"eng"}],"language":[{"iso":"eng"}],"keyword":["Chemistry (miscellaneous)","Analytical Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Molecular Medicine","Drug Discovery","Pharmaceutical Science"],"publication_identifier":{"issn":["1420-3049"]},"publication_status":"published","intvolume":"        28","citation":{"ama":"Huang J, Qiu Y, Lücke F, Su J, Grundmeier G, Keller A. Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy. <i>Molecules</i>. 2023;28(16). doi:<a href=\"https://doi.org/10.3390/molecules28166060\">10.3390/molecules28166060</a>","ieee":"J. Huang, Y. Qiu, F. Lücke, J. Su, G. Grundmeier, and A. Keller, “Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy,” <i>Molecules</i>, vol. 28, no. 16, Art. no. 6060, 2023, doi: <a href=\"https://doi.org/10.3390/molecules28166060\">10.3390/molecules28166060</a>.","chicago":"Huang, Jingyuan, Yunshu Qiu, Felix Lücke, Jiangling Su, Guido Grundmeier, and Adrian Keller. “Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy.” <i>Molecules</i> 28, no. 16 (2023). <a href=\"https://doi.org/10.3390/molecules28166060\">https://doi.org/10.3390/molecules28166060</a>.","apa":"Huang, J., Qiu, Y., Lücke, F., Su, J., Grundmeier, G., &#38; Keller, A. (2023). Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy. <i>Molecules</i>, <i>28</i>(16), Article 6060. <a href=\"https://doi.org/10.3390/molecules28166060\">https://doi.org/10.3390/molecules28166060</a>","short":"J. Huang, Y. Qiu, F. Lücke, J. Su, G. Grundmeier, A. Keller, Molecules 28 (2023).","mla":"Huang, Jingyuan, et al. “Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy.” <i>Molecules</i>, vol. 28, no. 16, 6060, MDPI AG, 2023, doi:<a href=\"https://doi.org/10.3390/molecules28166060\">10.3390/molecules28166060</a>.","bibtex":"@article{Huang_Qiu_Lücke_Su_Grundmeier_Keller_2023, title={Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy}, volume={28}, DOI={<a href=\"https://doi.org/10.3390/molecules28166060\">10.3390/molecules28166060</a>}, number={166060}, journal={Molecules}, publisher={MDPI AG}, author={Huang, Jingyuan and Qiu, Yunshu and Lücke, Felix and Su, Jiangling and Grundmeier, Guido and Keller, Adrian}, year={2023} }"},"volume":28,"author":[{"first_name":"Jingyuan","full_name":"Huang, Jingyuan","last_name":"Huang"},{"last_name":"Qiu","full_name":"Qiu, Yunshu","first_name":"Yunshu"},{"full_name":"Lücke, Felix","last_name":"Lücke","first_name":"Felix"},{"full_name":"Su, Jiangling","last_name":"Su","first_name":"Jiangling"},{"id":"194","full_name":"Grundmeier, Guido","last_name":"Grundmeier","first_name":"Guido"},{"first_name":"Adrian","id":"48864","full_name":"Keller, Adrian","orcid":"0000-0001-7139-3110","last_name":"Keller"}],"date_updated":"2023-08-16T10:53:08Z","doi":"10.3390/molecules28166060","type":"journal_article","status":"public","department":[{"_id":"302"}],"user_id":"48864","_id":"46542","article_number":"6060"}]