@article{51121,
  abstract     = {{<jats:p>DNA origami nanostructures are a powerful tool in biomedicine and can be used to combat drug‐resistant bacterial infections. However, the effect of unmodified DNA origami nanostructures on bacteria is yet to be elucidated. With the aim to obtain a better understanding of this phenomenon, the effect of three DNA origami shapes, i.e., DNA origami triangles, six‐helix bundles (6HBs), and 24‐helix bundles (24HBs), on the growth of Gram‐negative Escherichia coli and Gram‐positive Bacillus subtilis is investigated. These results reveal that while triangles and 24HBs can be used as a source of nutrients by E. coli and thereby promote population growth, their effect is much smaller than that of genomic single‐ and double‐stranded DNA. However, no effect on E. coli population growth is observed for the 6HBs. On the other hand, B. subtilis does not show any significant changes in population growth when cultured with the different DNA origami shapes or genomic DNA. The detailed effect of DNA origami nanostructures on bacterial growth thus depends on the competence signals and uptake mechanism of each bacterial species, as well as the DNA origami shape. This should be considered in the development of antimicrobial DNA origami nanostructures.</jats:p>}},
  author       = {{Garcia-Diosa, Jaime Andres and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1439-4227}},
  journal      = {{ChemBioChem}},
  keywords     = {{Organic Chemistry, Molecular Biology, Molecular Medicine, Biochemistry}},
  publisher    = {{Wiley}},
  title        = {{{Effect of DNA Origami Nanostructures on Bacterial Growth}}},
  doi          = {{10.1002/cbic.202400091}},
  year         = {{2024}},
}

@article{52541,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>We conducted an investigation into the palladium‐catalyzed carbon‐sulfur cross‐coupling reaction involving a 2‐bromothiophene derivative and potassium thioacetate as a substitute for hydrogen sulfide. This investigation utilized kinetic and computational methods. We synthesized two palladium complexes supported by the bisphosphane ligands bis(diphenylphosphino)ferrocene (DPPF) and bis(diisopropylphosphino)ferrocene (D<jats:italic>i</jats:italic>PPF), as well as their tentative intermediates in the catalytic cycle. Reaction rates were measured and then compared to computational predictions.</jats:p>}},
  author       = {{Peschtrich, Sebastian and Schoch, Roland and Kuckling, Dirk and Paradies, Jan}},
  issn         = {{1434-193X}},
  journal      = {{European Journal of Organic Chemistry}},
  keywords     = {{Organic Chemistry, Physical and Theoretical Chemistry}},
  number       = {{8}},
  publisher    = {{Wiley}},
  title        = {{{A Comparative Kinetic and Computational Investigation of the Carbon‐Sulfur Cross Coupling of Potassium Thioacetate and 2‐Bromo Thiophene Using Palladium/Bisphosphine Complexes}}},
  doi          = {{10.1002/ejoc.202301207}},
  volume       = {{27}},
  year         = {{2024}},
}

@article{53163,
  abstract     = {{<jats:p>An SPR-based dually crosslinked gel sensor for adiponitrile bearing pillar[5]arene responsive sites with a low limit of detection was developed.</jats:p>}},
  author       = {{Rodin, Maksim and Helle, David and Kuckling, Dirk}},
  issn         = {{1759-9954}},
  journal      = {{Polymer Chemistry}},
  keywords     = {{Organic Chemistry, Polymers and Plastics, Biochemistry, Bioengineering}},
  number       = {{7}},
  pages        = {{661--679}},
  publisher    = {{Royal Society of Chemistry (RSC)}},
  title        = {{{Pillar[5]arene-based dually crosslinked supramolecular gel as a sensor for the detection of adiponitrile}}},
  doi          = {{10.1039/d3py01354e}},
  volume       = {{15}},
  year         = {{2024}},
}

@article{48588,
  abstract     = {{<jats:p>Bacterial colonization and biofilm formation on abiotic surfaces are initiated by the adhesion of peptides and proteins. Understanding the adhesion of such peptides and proteins at a molecular level thus represents an important step toward controlling and suppressing biofilm formation on technological and medical materials. This study investigates the molecular adhesion of a pilus‐derived peptide that facilitates biofilm formation of Pseudomonas aeruginosa, a multidrug‐resistant opportunistic pathogen frequently encountered in healthcare settings. Single‐molecule force spectroscopy (SMFS) was performed on chemically etched ZnO surfaces to gather insights about peptide adsorption force and its kinetics. Metal‐free click chemistry for the fabrication of peptide‐terminated SMFS cantilevers was performed on amine‐terminated gold cantilevers and verified by X‐ray photoelectron spectroscopy (XPS) and polarization‐modulated infrared reflection absorption spectroscopy (PM‐IRRAS). Atomic force microscopy (AFM) and XPS analyses reveal stable topographies and surface chemistries of the substrates that are not affected by SMFS. Rupture events described by the worm‐like chain model (WLC) up to 600 pN were detected for the non‐polar ZnO(11‐20) surfaces. The dissociation barrier energy at zero force ΔG(0), the transition state distance xb and bound‐unbound dissociation rate at zero force koff(0) for the single crystalline substrate indicate that coordination and hydrogen bonds dominate the peptide/surface interaction.</jats:p>}},
  author       = {{Prüßner, Tim and Meinderink, Dennis and Zhu, Siqi and Orive, Alejandro G. and Kielar, Charlotte and Huck, Marten and Steinrück, Hans-Georg and Keller, Adrian and Grundmeier, Guido}},
  issn         = {{0947-6539}},
  journal      = {{Chemistry – A European Journal}},
  keywords     = {{General Chemistry, Catalysis, Organic Chemistry}},
  publisher    = {{Wiley}},
  title        = {{{Molecular Adhesion of a Pilus‐derived Peptide Involved in Pseudomonas aeruginosa Biofilm Formation on non‐polar ZnO Surfaces}}},
  doi          = {{10.1002/chem.202302464}},
  year         = {{2023}},
}

@article{50150,
  abstract     = {{<jats:p>Covalent peptidomimetic protease inhibitors have gained a lot of attention in drug development in recent years. They are designed to covalently bind the catalytically active amino acids through electrophilic groups called warheads. Covalent inhibition has an advantage in terms of pharmacodynamic properties but can also bear toxicity risks due to non-selective off-target protein binding. Therefore, the right combination of a reactive warhead with a well-suited peptidomimetic sequence is of great importance. Herein, the selectivities of well-known warheads combined with peptidomimetic sequences suited for five different proteases were investigated, highlighting the impact of both structure parts (warhead and peptidomimetic sequence) for affinity and selectivity. Molecular docking gave insights into the predicted binding modes of the inhibitors inside the binding pockets of the different enzymes. Moreover, the warheads were investigated by NMR and LC-MS reactivity assays against serine/threonine and cysteine nucleophile models, as well as by quantum mechanics simulations.</jats:p>}},
  author       = {{Müller, Patrick and Meta, Mergim and Meidner, Jan Laurenz and Schwickert, Marvin and Meyr, Jessica and Schwickert, Kevin and Kersten, Christian and Zimmer, Collin and Hammerschmidt, Stefan Josef and Frey, Ariane and Lahu, Albin and de la Hoz-Rodríguez, Sergio and Agost-Beltrán, Laura and Rodríguez, Santiago and Diemer, Kira and Neumann, Wilhelm and Gonzàlez, Florenci V. and Engels, Bernd and Schirmeister, Tanja}},
  issn         = {{1422-0067}},
  journal      = {{International Journal of Molecular Sciences}},
  keywords     = {{Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis}},
  number       = {{8}},
  publisher    = {{MDPI AG}},
  title        = {{{Investigation of the Compatibility between Warheads and Peptidomimetic Sequences of Protease Inhibitors—A Comprehensive Reactivity and Selectivity Study}}},
  doi          = {{10.3390/ijms24087226}},
  volume       = {{24}},
  year         = {{2023}},
}

@article{46023,
  abstract     = {{<jats:p>This article presents the potential-dependent adsorption of two proteins, bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and 37 °C. The adsorption process was studied on an electropolished alloy under cathodic and anodic overpotentials, compared to the open circuit potential (OCP). To analyze the adsorption process, various complementary interface analytical techniques were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy), and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization experiments were conducted within a potential range where charging of the electric double layer dominates, and Faradaic currents can be disregarded. The findings highlight the significant influence of the interfacial charge distribution on the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical analysis of the protein layers formed under applied overpotentials demonstrated improved corrosion protection properties. These studies provide valuable insights into protein adsorption on titanium alloys under physiological conditions, characterized by varying potentials of the passive alloy.</jats:p>}},
  author       = {{Duderija, Belma and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and Keller, Adrian and Grundmeier, Guido}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  keywords     = {{Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science}},
  number       = {{13}},
  publisher    = {{MDPI AG}},
  title        = {{{Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy}}},
  doi          = {{10.3390/molecules28135109}},
  volume       = {{28}},
  year         = {{2023}},
}

@article{48277,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Currently, the fused deposition modeling (FDM) process is the most common additive manufacturing technology. The principle of the FDM process is the strand wise deposition of molten thermoplastic polymers, by feeding a filament trough a heated nozzle. Due to the strand and layer wise deposition the cooling of the manufactured component is not uniform. This leads to dimensional deviations which may cause the component to be unusable for the desired application. In this paper, a method is described which is based on the shrinkage compensation through the adaption of every single raster line in components manufactured with the FDM process. The shrinkage compensation is based on a model resulting from a DOE which considers the main influencing factors on the shrinkage behavior of raster lines in the FDM process. An in‐house developed software analyzes the component and locally applies the shrinkage compensation with consideration of the boundary conditions, e.g., the position of the raster line in the component and the process parameters. Following, a validation using a simple geometry is conducted to show the effect of the presented adaptive scaling method.</jats:p>}},
  author       = {{Moritzer, Elmar and Hecker, Felix}},
  issn         = {{1022-1360}},
  journal      = {{Macromolecular Symposia}},
  keywords     = {{Materials Chemistry, Polymers and Plastics, Organic Chemistry, Condensed Matter Physics}},
  location     = {{Bukarest}},
  number       = {{1}},
  publisher    = {{Wiley}},
  title        = {{{Adaptive Scaling of Components in the Fused Deposition Modeling Process}}},
  doi          = {{10.1002/masy.202200181}},
  volume       = {{411}},
  year         = {{2023}},
}

@article{52542,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>We report on so‐called “hidden FLPs” (FLP: frustrated Lewis pair) consisting of a phosphorus ylide featuring a group 13 fragment in the <jats:italic>ortho</jats:italic> position of a phenyl ring scaffold to form five‐membered ring structures. Although the formation of the Lewis acid/base adducts was observed in the solid state, most of the title compounds readily react with carbon dioxide to provide stable insertion products. Strikingly, 0.3–3.0 mol% of the reported aluminum and gallium/carbon‐based ambiphiles catalyze the reduction of CO<jats:sub>2</jats:sub> to methanol with satisfactory high selectivity and yields using pinacol borane as stoichiometric reduction equivalent. Comprehensive computational studies provided valuable mechanistic insights and shed more light on activity differences.</jats:p>}},
  author       = {{Krämer, Felix and Paradies, Jan and Fernández, Israel and Breher, Frank}},
  issn         = {{0947-6539}},
  journal      = {{Chemistry – A European Journal}},
  keywords     = {{General Chemistry, Catalysis, Organic Chemistry}},
  number       = {{5}},
  publisher    = {{Wiley}},
  title        = {{{Quo Vadis CO<sub>2</sub> Activation: Catalytic Reduction of CO<sub>2</sub> to Methanol Using Aluminum and Gallium/Carbon‐based Ambiphiles}}},
  doi          = {{10.1002/chem.202303380}},
  volume       = {{30}},
  year         = {{2023}},
}

@article{52802,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Currently, the fused deposition modeling (FDM) process is the most common additive manufacturing technology. The principle of the FDM process is the strand wise deposition of molten thermoplastic polymers, by feeding a filament trough a heated nozzle. Due to the strand and layer wise deposition the cooling of the manufactured component is not uniform. This leads to dimensional deviations which may cause the component to be unusable for the desired application. In this paper, a method is described which is based on the shrinkage compensation through the adaption of every single raster line in components manufactured with the FDM process. The shrinkage compensation is based on a model resulting from a DOE which considers the main influencing factors on the shrinkage behavior of raster lines in the FDM process. An in‐house developed software analyzes the component and locally applies the shrinkage compensation with consideration of the boundary conditions, e.g., the position of the raster line in the component and the process parameters. Following, a validation using a simple geometry is conducted to show the effect of the presented adaptive scaling method.</jats:p>}},
  author       = {{Moritzer, Elmar and Hecker, Felix}},
  issn         = {{1022-1360}},
  journal      = {{Macromolecular Symposia}},
  keywords     = {{Materials Chemistry, Polymers and Plastics, Organic Chemistry, Condensed Matter Physics}},
  number       = {{1}},
  publisher    = {{Wiley}},
  title        = {{{Adaptive Scaling of Components in the Fused Deposition Modeling Process}}},
  doi          = {{10.1002/masy.202200181}},
  volume       = {{411}},
  year         = {{2023}},
}

@article{53170,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Coating medical implants with antibacterial polymers may prevent postoperative infections which are a common issue for conventional titanium implants and can even lead to implant failure. Easily applicable diblock copolymers are presented that form polymer brushes via “grafting to” mechanism on titanium and equip the modified material with antibacterial properties. The polymers carry quaternized pyridinium units to combat bacteria and phosphonic acid groups which allow the linear chains to be anchored to metal surfaces in a convenient coating process. The polymers are synthesized via reversible‐addition‐fragmentation‐chain‐transfer (RAFT) polymerization and postmodifications and are characterized using NMR spectroscopy and SEC. Low grafting densities are a major drawback of the “grafting to” approach compared to “grafting from”. Thus, the number of phosphonic acid groups in the anchor block are varied to investigate and optimize the surface binding. Modified titanium surfaces are examined regarding their composition, wetting behavior, streaming potential, and coating stability. Evaluation of the antimicrobial properties revealed reduced bacterial adhesion and biofilm formation for certain polymers, albeit the cell biocompatibility against human gingival fibroblasts is also impaired. The presented findings show the potential of easy‐to‐apply polymer coatings and aid in designing next‐generation implant surface modifications.</jats:p>}},
  author       = {{Methling, Rafael and Dückmann, Oliver and Simon, Frank and Wolf‐Brandstetter, Cornelia and Kuckling, Dirk}},
  issn         = {{1438-7492}},
  journal      = {{Macromolecular Materials and Engineering}},
  keywords     = {{Materials Chemistry, Polymers and Plastics, Organic Chemistry, General Chemical Engineering}},
  number       = {{8}},
  publisher    = {{Wiley}},
  title        = {{{Antimicrobial Brushes on Titanium via “Grafting to” Using Phosphonic Acid/Pyridinium Containing Block Copolymers}}},
  doi          = {{10.1002/mame.202200665}},
  volume       = {{308}},
  year         = {{2023}},
}

@article{52344,
  abstract     = {{Macrocyclization reactions are still challenging due to competing oligomerization, which requires the use of small substrate concentrations. Here, the cationic tungsten imido and tungsten oxo alkylidene N-heterocyclic carbene complexes [[W(N-2,6-Cl2-C6H3)(CHCMe2Ph(OC6F5)(pivalonitrile)(IMes)+ B(ArF)4−] (W1) and [W(O)(CHCMe2Ph(OCMe(CF3)2)(IMes)(CH3CN)+ B(ArF)4−] (W2) (IMes=1,3-dimesitylimidazol-2-ylidene; B(ArF)4−=tetrakis(3,5-bis(trifluoromethyl)phenyl borate) have been immobilized inside the pores of ordered mesoporous silica (OMS) with pore diameters of 3.3 and 6.8 nm, respectively, using a pore-selective immobilization protocol. X-ray absorption spectroscopy of W1@OMS showed that even though the catalyst structure is contracted due to confinement by the mesopores, both the oxidation state and structure of the catalyst stayed intact upon immobilization. Catalytic testing with four differently sized α,ω-dienes revealed a dramatically increased macrocyclization (MC) and Z-selectivity of the supported catalysts compared to the homogenous progenitors, allowing high substrate concentrations of 25 mM. With the supported complexes, a maximum increase in MC-selectivity from 27 to 81 % and in Z-selectivity from 17 to 34 % was achieved. In general, smaller mesopores exhibited a stronger confinement effect. A comparison of the two supported tungsten-based catalysts showed that W1@OMS possesses a higher MC-selectivity, while W2@OMS exhibits a higher Z-selectivity which can be rationalized by the structures of the catalysts.}},
  author       = {{Ziegler, Felix and Bruckner, Johanna R. and Nowakowski, Michał and Bauer, Matthias and Probst, Patrick and Atwi, Boshra and Buchmeiser, Michael R.}},
  issn         = {{1867-3880}},
  journal      = {{ChemCatChem}},
  keywords     = {{Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Catalysis}},
  number       = {{21}},
  publisher    = {{Wiley}},
  title        = {{{Macrocyclization of Dienes under Confinement with Cationic Tungsten Imido/Oxo Alkylidene <i>N</i>‐Heterocyclic Carbene Complexes}}},
  doi          = {{10.1002/cctc.202300871}},
  volume       = {{15}},
  year         = {{2023}},
}

@article{33447,
  author       = {{Julin, Sofia and Keller, Adrian and Linko, Veikko}},
  issn         = {{1043-1802}},
  journal      = {{Bioconjugate Chemistry}},
  keywords     = {{Organic Chemistry, Pharmaceutical Science, Pharmacology, Biomedical Engineering, Bioengineering, Biotechnology}},
  pages        = {{18--29}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Dynamics of DNA Origami Lattices}}},
  doi          = {{10.1021/acs.bioconjchem.2c00359}},
  volume       = {{34}},
  year         = {{2023}},
}

@article{35693,
  abstract     = {{<jats:p>In recent years, frustrated Lewis pairs have been widely used in small molecules activation and catalytic transformations. This graphic review is aimed to provide the fundamental understanding of frustrated Lewis pair reactivity and the exploitation thereof in catalytic reactions.</jats:p>}},
  author       = {{Zhou, Rundong and Tavandashti, Zoleykha and Paradies, Jan}},
  issn         = {{2509-9396}},
  journal      = {{SynOpen}},
  keywords     = {{Organic Chemistry, Materials Science (miscellaneous), Biomaterials, Catalysis}},
  publisher    = {{Georg Thieme Verlag KG}},
  title        = {{{Frustrated Lewis Pair Catalysed Reactions}}},
  doi          = {{10.1055/a-2005-5443}},
  year         = {{2023}},
}

@article{42953,
  author       = {{Cara, Eleonora and Hönicke, Philipp and Kayser, Yves and Lindner, Jörg K. N. and Castellino, Micaela and Murataj, Irdi and Porro, Samuele and Angelini, Angelo and De Leo, Natascia and Pirri, Candido Fabrizio and Beckhoff, Burkhard and Boarino, Luca and Ferrarese Lupi, Federico}},
  issn         = {{2637-6105}},
  journal      = {{ACS Applied Polymer Materials}},
  keywords     = {{Organic Chemistry, Polymers and Plastics, Process Chemistry and Technology}},
  number       = {{3}},
  pages        = {{2079--2087}},
  publisher    = {{American Chemical Society (ACS)}},
  title        = {{{Developing Quantitative Nondestructive Characterization of Nanomaterials: A Case Study on Sequential Infiltration Synthesis of Block Copolymers}}},
  doi          = {{10.1021/acsapm.2c02094}},
  volume       = {{5}},
  year         = {{2023}},
}

@article{44503,
  author       = {{Hanke, Marcel and Tomm, Emilia and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1439-4227}},
  journal      = {{ChemBioChem}},
  keywords     = {{Organic Chemistry, Molecular Biology, Molecular Medicine, Biochemistry}},
  publisher    = {{Wiley}},
  title        = {{{Effect of Ionic Strength on the Thermal Stability of DNA Origami Nanostructures}}},
  doi          = {{10.1002/cbic.202300338}},
  year         = {{2023}},
}

@article{43827,
  abstract     = {{A series of new organic donor–π–acceptor dyes incorporating a diquat moiety as a novel electron-acceptor unit have been synthesized and characterized. The analytical data were supported by DFT calculations. These dyes were explored in the aerobic thiocyanation of indoles and pyrroles. Here they showed a high photocatalytic activity under visible light, giving isolated yields of up to 97 %. In addition, the photocatalytic activity of standalone diquat and methyl viologen through formation of an electron donor acceptor complex is presented.}},
  author       = {{Meier, Armin and Badalov, Sabuhi and Biktagirov, Timur and Schmidt, Wolf Gero and Wilhelm, René}},
  issn         = {{0947-6539}},
  journal      = {{Chemistry – A European Journal}},
  keywords     = {{General Chemistry, Catalysis, Organic Chemistry}},
  number       = {{22}},
  pages        = {{ e202203541}},
  publisher    = {{Wiley}},
  title        = {{{Diquat Based Dyes: A New Class of Photoredox Catalysts and Their Use in Aerobic Thiocyanation}}},
  doi          = {{10.1002/chem.202203541}},
  volume       = {{ 29}},
  year         = {{2023}},
}

@article{45828,
  abstract     = {{<jats:p>This article presents the potential-dependent adsorption of two proteins, bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and 37 °C. The adsorption process was studied on an electropolished alloy under cathodic and anodic overpotentials, compared to the open circuit potential (OCP). To analyze the adsorption process, various complementary interface analytical techniques were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy), and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization experiments were conducted within a potential range where charging of the electric double layer dominates, and Faradaic currents can be disregarded. The findings highlight the significant influence of the interfacial charge distribution on the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical analysis of the protein layers formed under applied overpotentials demonstrated improved corrosion protection properties. These studies provide valuable insights into protein adsorption on titanium alloys under physiological conditions, characterized by varying potentials of the passive alloy.</jats:p>}},
  author       = {{Duderija, Belma and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and Keller, Adrian and Grundmeier, Guido}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  keywords     = {{Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science}},
  number       = {{13}},
  pages        = {{5109}},
  publisher    = {{MDPI AG}},
  title        = {{{Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy}}},
  doi          = {{10.3390/molecules28135109}},
  volume       = {{28}},
  year         = {{2023}},
}

@article{46542,
  abstract     = {{<jats:p>Multiprotein adsorption from complex body fluids represents a highly important and complicated phenomenon in medicine. In this work, multiprotein adsorption from diluted human serum at gold and oxidized iron surfaces is investigated at different serum concentrations and pH values. Adsorption-induced changes in surface topography and the total amount of adsorbed proteins are quantified by atomic force microscopy (AFM) and polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS), respectively. For both surfaces, stronger protein adsorption is observed at pH 6 compared to pH 7 and pH 8. PM-IRRAS furthermore provides some qualitative insights into the pH-dependent alterations in the composition of the adsorbed multiprotein films. Changes in the amide II/amide I band area ratio and in particular side-chain IR absorption suggest that the increased adsorption at pH 6 is accompanied by a change in protein film composition. Presumably, this is mostly driven by the adsorption of human serum albumin, which at pH 6 adsorbs more readily and thereby replaces other proteins with lower surface affinities in the resulting multiprotein film.</jats:p>}},
  author       = {{Huang, Jingyuan and Qiu, Yunshu and Lücke, Felix and Su, Jiangling and Grundmeier, Guido and Keller, Adrian}},
  issn         = {{1420-3049}},
  journal      = {{Molecules}},
  keywords     = {{Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science}},
  number       = {{16}},
  publisher    = {{MDPI AG}},
  title        = {{{Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy}}},
  doi          = {{10.3390/molecules28166060}},
  volume       = {{28}},
  year         = {{2023}},
}

@article{46543,
  abstract     = {{<jats:p>The influence of nanoscale surface topography on protein adsorption is highly important for numerous applications in medicine and technology. Herein, ferritin adsorption at flat and nanofaceted, single-crystalline Al2O3 surfaces is investigated using atomic force microscopy and X-ray photoelectron spectroscopy. The nanofaceted surfaces are generated by the thermal annealing of Al2O3 wafers at temperatures above 1000 °C, which leads to the formation of faceted saw-tooth-like surface topographies with periodicities of about 160 nm and amplitudes of about 15 nm. Ferritin adsorption at these nanofaceted surfaces is notably suppressed compared to the flat surface at a concentration of 10 mg/mL, which is attributed to lower adsorption affinities of the newly formed facets. Consequently, adsorption is restricted mostly to the pattern grooves, where the proteins can maximize their contact area with the surface. However, this effect depends on the protein concentration, with an inverse trend being observed at 30 mg/mL. Furthermore, different ferritin adsorption behavior is observed at topographically similar nanofacet patterns fabricated at different annealing temperatures and attributed to different step and kink densities. These results demonstrate that while protein adsorption at solid surfaces can be notably affected by nanofacet patterns, fine-tuning protein adsorption in this way requires the precise control of facet properties.</jats:p>}},
  author       = {{Pothineni, Bhanu K. and Kollmann, Sabrina and Li, Xinyang and Grundmeier, Guido and Erb, Denise J. and Keller, Adrian}},
  issn         = {{1422-0067}},
  journal      = {{International Journal of Molecular Sciences}},
  keywords     = {{Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis}},
  number       = {{16}},
  publisher    = {{MDPI AG}},
  title        = {{{Adsorption of Ferritin at Nanofaceted Al2O3 Surfaces}}},
  doi          = {{10.3390/ijms241612808}},
  volume       = {{24}},
  year         = {{2023}},
}

@article{30209,
  abstract     = {{<jats:p>DNA origami technology enables the folding of DNA strands into complex nanoscale shapes whose properties and interactions with molecular species often deviate significantly from that of genomic DNA. Here, we investigate the salting-out of different DNA origami shapes by the kosmotropic salt ammonium sulfate that is routinely employed in protein precipitation. We find that centrifugation in the presence of 3 M ammonium sulfate results in notable precipitation of DNA origami nanostructures but not of double-stranded genomic DNA. The precipitated DNA origami nanostructures can be resuspended in ammonium sulfate-free buffer without apparent formation of aggregates or loss of structural integrity. Even though quasi-1D six-helix bundle DNA origami are slightly less susceptible toward salting-out than more compact DNA origami triangles and 24-helix bundles, precipitation and recovery yields appear to be mostly independent of DNA origami shape and superstructure. Exploiting the specificity of ammonium sulfate salting-out for DNA origami nanostructures, we further apply this method to separate DNA origami triangles from genomic DNA fragments in a complex mixture. Our results thus demonstrate the possibility of concentrating and purifying DNA origami nanostructures by ammonium sulfate-induced salting-out.</jats:p>}},
  author       = {{Hanke, Marcel and Hansen, Niklas and Chen, Ruiping and Grundmeier, Guido and Fahmy, Karim and Keller, Adrian}},
  issn         = {{1422-0067}},
  journal      = {{International Journal of Molecular Sciences}},
  keywords     = {{Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis}},
  number       = {{5}},
  pages        = {{2817}},
  publisher    = {{MDPI AG}},
  title        = {{{Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate}}},
  doi          = {{10.3390/ijms23052817}},
  volume       = {{23}},
  year         = {{2022}},
}