---
_id: '46023'
abstract:
- lang: eng
  text: <jats:p>This article presents the potential-dependent adsorption of two proteins,
    bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and
    37 °C. The adsorption process was studied on an electropolished alloy under cathodic
    and anodic overpotentials, compared to the open circuit potential (OCP). To analyze
    the adsorption process, various complementary interface analytical techniques
    were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption
    spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy),
    and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization
    experiments were conducted within a potential range where charging of the electric
    double layer dominates, and Faradaic currents can be disregarded. The findings
    highlight the significant influence of the interfacial charge distribution on
    the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical
    analysis of the protein layers formed under applied overpotentials demonstrated
    improved corrosion protection properties. These studies provide valuable insights
    into protein adsorption on titanium alloys under physiological conditions, characterized
    by varying potentials of the passive alloy.</jats:p>
article_number: '5109'
author:
- first_name: Belma
  full_name: Duderija, Belma
  last_name: Duderija
- first_name: Alejandro
  full_name: González-Orive, Alejandro
  last_name: González-Orive
- first_name: Christoph
  full_name: Ebbert, Christoph
  last_name: Ebbert
- first_name: Vanessa
  full_name: Neßlinger, Vanessa
  last_name: Neßlinger
- first_name: Adrian
  full_name: Keller, Adrian
  last_name: Keller
- first_name: Guido
  full_name: Grundmeier, Guido
  last_name: Grundmeier
citation:
  ama: Duderija B, González-Orive A, Ebbert C, Neßlinger V, Keller A, Grundmeier G.
    Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.
    <i>Molecules</i>. 2023;28(13). doi:<a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>
  apa: Duderija, B., González-Orive, A., Ebbert, C., Neßlinger, V., Keller, A., &#38;
    Grundmeier, G. (2023). Electrode Potential-Dependent Studies of Protein Adsorption
    on Ti6Al4V Alloy. <i>Molecules</i>, <i>28</i>(13), Article 5109. <a href="https://doi.org/10.3390/molecules28135109">https://doi.org/10.3390/molecules28135109</a>
  bibtex: '@article{Duderija_González-Orive_Ebbert_Neßlinger_Keller_Grundmeier_2023,
    title={Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V
    Alloy}, volume={28}, DOI={<a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>},
    number={135109}, journal={Molecules}, publisher={MDPI AG}, author={Duderija, Belma
    and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and
    Keller, Adrian and Grundmeier, Guido}, year={2023} }'
  chicago: Duderija, Belma, Alejandro González-Orive, Christoph Ebbert, Vanessa Neßlinger,
    Adrian Keller, and Guido Grundmeier. “Electrode Potential-Dependent Studies of
    Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i> 28, no. 13 (2023). <a href="https://doi.org/10.3390/molecules28135109">https://doi.org/10.3390/molecules28135109</a>.
  ieee: 'B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, and G.
    Grundmeier, “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V
    Alloy,” <i>Molecules</i>, vol. 28, no. 13, Art. no. 5109, 2023, doi: <a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>.'
  mla: Duderija, Belma, et al. “Electrode Potential-Dependent Studies of Protein Adsorption
    on Ti6Al4V Alloy.” <i>Molecules</i>, vol. 28, no. 13, 5109, MDPI AG, 2023, doi:<a
    href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>.
  short: B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, G. Grundmeier,
    Molecules 28 (2023).
date_created: 2023-07-12T07:55:40Z
date_updated: 2024-02-06T12:33:55Z
department:
- _id: '321'
- _id: '302'
doi: 10.3390/molecules28135109
intvolume: '        28'
issue: '13'
keyword:
- Chemistry (miscellaneous)
- Analytical Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Molecular Medicine
- Drug Discovery
- Pharmaceutical Science
language:
- iso: eng
publication: Molecules
publication_identifier:
  issn:
  - 1420-3049
publication_status: published
publisher: MDPI AG
status: public
title: Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy
type: journal_article
user_id: '54863'
volume: 28
year: '2023'
...
---
_id: '33447'
author:
- first_name: Sofia
  full_name: Julin, Sofia
  last_name: Julin
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
- first_name: Veikko
  full_name: Linko, Veikko
  last_name: Linko
citation:
  ama: Julin S, Keller A, Linko V. Dynamics of DNA Origami Lattices. <i>Bioconjugate
    Chemistry</i>. 2023;34:18-29. doi:<a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">10.1021/acs.bioconjchem.2c00359</a>
  apa: Julin, S., Keller, A., &#38; Linko, V. (2023). Dynamics of DNA Origami Lattices.
    <i>Bioconjugate Chemistry</i>, <i>34</i>, 18–29. <a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">https://doi.org/10.1021/acs.bioconjchem.2c00359</a>
  bibtex: '@article{Julin_Keller_Linko_2023, title={Dynamics of DNA Origami Lattices},
    volume={34}, DOI={<a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">10.1021/acs.bioconjchem.2c00359</a>},
    journal={Bioconjugate Chemistry}, publisher={American Chemical Society (ACS)},
    author={Julin, Sofia and Keller, Adrian and Linko, Veikko}, year={2023}, pages={18–29}
    }'
  chicago: 'Julin, Sofia, Adrian Keller, and Veikko Linko. “Dynamics of DNA Origami
    Lattices.” <i>Bioconjugate Chemistry</i> 34 (2023): 18–29. <a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">https://doi.org/10.1021/acs.bioconjchem.2c00359</a>.'
  ieee: 'S. Julin, A. Keller, and V. Linko, “Dynamics of DNA Origami Lattices,” <i>Bioconjugate
    Chemistry</i>, vol. 34, pp. 18–29, 2023, doi: <a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">10.1021/acs.bioconjchem.2c00359</a>.'
  mla: Julin, Sofia, et al. “Dynamics of DNA Origami Lattices.” <i>Bioconjugate Chemistry</i>,
    vol. 34, American Chemical Society (ACS), 2023, pp. 18–29, doi:<a href="https://doi.org/10.1021/acs.bioconjchem.2c00359">10.1021/acs.bioconjchem.2c00359</a>.
  short: S. Julin, A. Keller, V. Linko, Bioconjugate Chemistry 34 (2023) 18–29.
date_created: 2022-09-19T07:44:24Z
date_updated: 2023-01-18T08:31:47Z
department:
- _id: '302'
doi: 10.1021/acs.bioconjchem.2c00359
intvolume: '        34'
keyword:
- Organic Chemistry
- Pharmaceutical Science
- Pharmacology
- Biomedical Engineering
- Bioengineering
- Biotechnology
language:
- iso: eng
page: 18-29
publication: Bioconjugate Chemistry
publication_identifier:
  issn:
  - 1043-1802
  - 1520-4812
publication_status: published
publisher: American Chemical Society (ACS)
status: public
title: Dynamics of DNA Origami Lattices
type: journal_article
user_id: '48864'
volume: 34
year: '2023'
...
---
_id: '45828'
abstract:
- lang: eng
  text: <jats:p>This article presents the potential-dependent adsorption of two proteins,
    bovine serum albumin (BSA) and lysozyme (LYZ), on Ti6Al4V alloy at pH 7.4 and
    37 °C. The adsorption process was studied on an electropolished alloy under cathodic
    and anodic overpotentials, compared to the open circuit potential (OCP). To analyze
    the adsorption process, various complementary interface analytical techniques
    were employed, including PM-IRRAS (polarization-modulation infrared reflection-absorption
    spectroscopy), AFM (atomic force microscopy), XPS (X-ray photoelectron spectroscopy),
    and E-QCM (electrochemical quartz crystal microbalance) measurements. The polarization
    experiments were conducted within a potential range where charging of the electric
    double layer dominates, and Faradaic currents can be disregarded. The findings
    highlight the significant influence of the interfacial charge distribution on
    the adsorption of BSA and LYZ onto the alloy surface. Furthermore, electrochemical
    analysis of the protein layers formed under applied overpotentials demonstrated
    improved corrosion protection properties. These studies provide valuable insights
    into protein adsorption on titanium alloys under physiological conditions, characterized
    by varying potentials of the passive alloy.</jats:p>
author:
- first_name: Belma
  full_name: Duderija, Belma
  id: '54863'
  last_name: Duderija
- first_name: Alejandro
  full_name: González-Orive, Alejandro
  last_name: González-Orive
- first_name: Christoph
  full_name: Ebbert, Christoph
  id: '7266'
  last_name: Ebbert
- first_name: Vanessa
  full_name: Neßlinger, Vanessa
  last_name: Neßlinger
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
citation:
  ama: Duderija B, González-Orive A, Ebbert C, Neßlinger V, Keller A, Grundmeier G.
    Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy.
    <i>Molecules</i>. 2023;28(13):5109. doi:<a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>
  apa: Duderija, B., González-Orive, A., Ebbert, C., Neßlinger, V., Keller, A., &#38;
    Grundmeier, G. (2023). Electrode Potential-Dependent Studies of Protein Adsorption
    on Ti6Al4V Alloy. <i>Molecules</i>, <i>28</i>(13), 5109. <a href="https://doi.org/10.3390/molecules28135109">https://doi.org/10.3390/molecules28135109</a>
  bibtex: '@article{Duderija_González-Orive_Ebbert_Neßlinger_Keller_Grundmeier_2023,
    title={Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V
    Alloy}, volume={28}, DOI={<a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>},
    number={13}, journal={Molecules}, publisher={MDPI AG}, author={Duderija, Belma
    and González-Orive, Alejandro and Ebbert, Christoph and Neßlinger, Vanessa and
    Keller, Adrian and Grundmeier, Guido}, year={2023}, pages={5109} }'
  chicago: 'Duderija, Belma, Alejandro González-Orive, Christoph Ebbert, Vanessa Neßlinger,
    Adrian Keller, and Guido Grundmeier. “Electrode Potential-Dependent Studies of
    Protein Adsorption on Ti6Al4V Alloy.” <i>Molecules</i> 28, no. 13 (2023): 5109.
    <a href="https://doi.org/10.3390/molecules28135109">https://doi.org/10.3390/molecules28135109</a>.'
  ieee: 'B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, and G.
    Grundmeier, “Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V
    Alloy,” <i>Molecules</i>, vol. 28, no. 13, p. 5109, 2023, doi: <a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>.'
  mla: Duderija, Belma, et al. “Electrode Potential-Dependent Studies of Protein Adsorption
    on Ti6Al4V Alloy.” <i>Molecules</i>, vol. 28, no. 13, MDPI AG, 2023, p. 5109,
    doi:<a href="https://doi.org/10.3390/molecules28135109">10.3390/molecules28135109</a>.
  short: B. Duderija, A. González-Orive, C. Ebbert, V. Neßlinger, A. Keller, G. Grundmeier,
    Molecules 28 (2023) 5109.
date_created: 2023-07-03T08:06:28Z
date_updated: 2023-07-03T08:07:55Z
department:
- _id: '302'
doi: 10.3390/molecules28135109
intvolume: '        28'
issue: '13'
keyword:
- Chemistry (miscellaneous)
- Analytical Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Molecular Medicine
- Drug Discovery
- Pharmaceutical Science
language:
- iso: eng
page: '5109'
publication: Molecules
publication_identifier:
  issn:
  - 1420-3049
publication_status: published
publisher: MDPI AG
status: public
title: Electrode Potential-Dependent Studies of Protein Adsorption on Ti6Al4V Alloy
type: journal_article
user_id: '48864'
volume: 28
year: '2023'
...
---
_id: '46542'
abstract:
- lang: eng
  text: <jats:p>Multiprotein adsorption from complex body fluids represents a highly
    important and complicated phenomenon in medicine. In this work, multiprotein adsorption
    from diluted human serum at gold and oxidized iron surfaces is investigated at
    different serum concentrations and pH values. Adsorption-induced changes in surface
    topography and the total amount of adsorbed proteins are quantified by atomic
    force microscopy (AFM) and polarization-modulation infrared reflection absorption
    spectroscopy (PM-IRRAS), respectively. For both surfaces, stronger protein adsorption
    is observed at pH 6 compared to pH 7 and pH 8. PM-IRRAS furthermore provides some
    qualitative insights into the pH-dependent alterations in the composition of the
    adsorbed multiprotein films. Changes in the amide II/amide I band area ratio and
    in particular side-chain IR absorption suggest that the increased adsorption at
    pH 6 is accompanied by a change in protein film composition. Presumably, this
    is mostly driven by the adsorption of human serum albumin, which at pH 6 adsorbs
    more readily and thereby replaces other proteins with lower surface affinities
    in the resulting multiprotein film.</jats:p>
article_number: '6060'
author:
- first_name: Jingyuan
  full_name: Huang, Jingyuan
  last_name: Huang
- first_name: Yunshu
  full_name: Qiu, Yunshu
  last_name: Qiu
- first_name: Felix
  full_name: Lücke, Felix
  last_name: Lücke
- first_name: Jiangling
  full_name: Su, Jiangling
  last_name: Su
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
citation:
  ama: Huang J, Qiu Y, Lücke F, Su J, Grundmeier G, Keller A. Multiprotein Adsorption
    from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic Force Microscopy
    and Polarization-Modulation Infrared Reflection Absorption Spectroscopy. <i>Molecules</i>.
    2023;28(16). doi:<a href="https://doi.org/10.3390/molecules28166060">10.3390/molecules28166060</a>
  apa: Huang, J., Qiu, Y., Lücke, F., Su, J., Grundmeier, G., &#38; Keller, A. (2023).
    Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied
    by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection Absorption
    Spectroscopy. <i>Molecules</i>, <i>28</i>(16), Article 6060. <a href="https://doi.org/10.3390/molecules28166060">https://doi.org/10.3390/molecules28166060</a>
  bibtex: '@article{Huang_Qiu_Lücke_Su_Grundmeier_Keller_2023, title={Multiprotein
    Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic
    Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy},
    volume={28}, DOI={<a href="https://doi.org/10.3390/molecules28166060">10.3390/molecules28166060</a>},
    number={166060}, journal={Molecules}, publisher={MDPI AG}, author={Huang, Jingyuan
    and Qiu, Yunshu and Lücke, Felix and Su, Jiangling and Grundmeier, Guido and Keller,
    Adrian}, year={2023} }'
  chicago: Huang, Jingyuan, Yunshu Qiu, Felix Lücke, Jiangling Su, Guido Grundmeier,
    and Adrian Keller. “Multiprotein Adsorption from Human Serum at Gold and Oxidized
    Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation Infrared
    Reflection Absorption Spectroscopy.” <i>Molecules</i> 28, no. 16 (2023). <a href="https://doi.org/10.3390/molecules28166060">https://doi.org/10.3390/molecules28166060</a>.
  ieee: 'J. Huang, Y. Qiu, F. Lücke, J. Su, G. Grundmeier, and A. Keller, “Multiprotein
    Adsorption from Human Serum at Gold and Oxidized Iron Surfaces Studied by Atomic
    Force Microscopy and Polarization-Modulation Infrared Reflection Absorption Spectroscopy,”
    <i>Molecules</i>, vol. 28, no. 16, Art. no. 6060, 2023, doi: <a href="https://doi.org/10.3390/molecules28166060">10.3390/molecules28166060</a>.'
  mla: Huang, Jingyuan, et al. “Multiprotein Adsorption from Human Serum at Gold and
    Oxidized Iron Surfaces Studied by Atomic Force Microscopy and Polarization-Modulation
    Infrared Reflection Absorption Spectroscopy.” <i>Molecules</i>, vol. 28, no. 16,
    6060, MDPI AG, 2023, doi:<a href="https://doi.org/10.3390/molecules28166060">10.3390/molecules28166060</a>.
  short: J. Huang, Y. Qiu, F. Lücke, J. Su, G. Grundmeier, A. Keller, Molecules 28
    (2023).
date_created: 2023-08-16T10:51:48Z
date_updated: 2023-08-16T10:53:08Z
department:
- _id: '302'
doi: 10.3390/molecules28166060
intvolume: '        28'
issue: '16'
keyword:
- Chemistry (miscellaneous)
- Analytical Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Molecular Medicine
- Drug Discovery
- Pharmaceutical Science
language:
- iso: eng
publication: Molecules
publication_identifier:
  issn:
  - 1420-3049
publication_status: published
publisher: MDPI AG
status: public
title: Multiprotein Adsorption from Human Serum at Gold and Oxidized Iron Surfaces
  Studied by Atomic Force Microscopy and Polarization-Modulation Infrared Reflection
  Absorption Spectroscopy
type: journal_article
user_id: '48864'
volume: 28
year: '2023'
...