---
_id: '65545'
abstract:
- lang: eng
  text: "<jats:title>ABSTRACT</jats:title>\r\n                  <jats:p>Ligation of
    staple strands in DNA origami nanostructures (DONs) can yield enhanced structural
    stability in critical environments. This process can be viewed as performing hundreds
    of parallel reactions programmed on a self‐assembled nanoscale platform. While
    previous studies have focused on investigating the collective results of the chemical
    or enzymatic ligation reactions, herein, the global quantitative analysis of individual
    ligation reactions is achieved using quantitative PCR (qPCR). By mapping enzymatic
    ligation efficiency on a trapezoidal substructure representing one‐third of a
    triangular DON, ligation is shown to preferentially occur at the trapezoid edges
    rather than at inner sites. Excellent agreement between the experimental ligation
    yields and docking simulations suggests that this is a result of variations in
    the ligase docking probability. Ligation products involving more than two consecutive
    sequences can be generated with each enzyme‐catalyzed reaction as an independent
    event. Interestingly, the sharp contrast between the edges vs. the inner sites
    has been abolished by changing the reaction conditions and performing the ligation
    in a DMSO co‐solvent system. This analytic method provides unprecedented insight
    into the multiple ligation reactions occurring in parallel within complex DONs
    and will be an invaluable tool in the translation of DONs from the lab to real‐world
    applications.</jats:p>"
article_number: e08136
author:
- first_name: Konrad
  full_name: Hacker, Konrad
  last_name: Hacker
- first_name: Emilia
  full_name: Juricke, Emilia
  id: '68157'
  last_name: Juricke
- first_name: Carolin
  full_name: Münch, Carolin
  last_name: Münch
- first_name: Antonio
  full_name: Suma, Antonio
  last_name: Suma
- first_name: Adrian Clemens
  full_name: Keller, Adrian Clemens
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
- first_name: Yixin
  full_name: Zhang, Yixin
  last_name: Zhang
citation:
  ama: Hacker K, Juricke E, Münch C, Suma A, Keller AC, Zhang Y. Global Quantitative
    Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures at the Single‐Nick
    Level. <i>Small</i>. Published online 2026. doi:<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>
  apa: Hacker, K., Juricke, E., Münch, C., Suma, A., Keller, A. C., &#38; Zhang, Y.
    (2026). Global Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA
    Nanostructures at the Single‐Nick Level. <i>Small</i>, Article e08136. <a href="https://doi.org/10.1002/smll.202508136">https://doi.org/10.1002/smll.202508136</a>
  bibtex: '@article{Hacker_Juricke_Münch_Suma_Keller_Zhang_2026, title={Global Quantitative
    Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures at the Single‐Nick
    Level}, DOI={<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>},
    number={e08136}, journal={Small}, publisher={Wiley}, author={Hacker, Konrad and
    Juricke, Emilia and Münch, Carolin and Suma, Antonio and Keller, Adrian Clemens
    and Zhang, Yixin}, year={2026} }'
  chicago: Hacker, Konrad, Emilia Juricke, Carolin Münch, Antonio Suma, Adrian Clemens
    Keller, and Yixin Zhang. “Global Quantitative Analysis of Ligation Reactions in
    Self‐Assembled DNA Nanostructures at the Single‐Nick Level.” <i>Small</i>, 2026.
    <a href="https://doi.org/10.1002/smll.202508136">https://doi.org/10.1002/smll.202508136</a>.
  ieee: 'K. Hacker, E. Juricke, C. Münch, A. Suma, A. C. Keller, and Y. Zhang, “Global
    Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures
    at the Single‐Nick Level,” <i>Small</i>, Art. no. e08136, 2026, doi: <a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>.'
  mla: Hacker, Konrad, et al. “Global Quantitative Analysis of Ligation Reactions
    in Self‐Assembled DNA Nanostructures at the Single‐Nick Level.” <i>Small</i>,
    e08136, Wiley, 2026, doi:<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>.
  short: K. Hacker, E. Juricke, C. Münch, A. Suma, A.C. Keller, Y. Zhang, Small (2026).
date_created: 2026-05-02T10:20:10Z
date_updated: 2026-05-02T10:20:35Z
department:
- _id: '302'
doi: 10.1002/smll.202508136
language:
- iso: eng
publication: Small
publication_identifier:
  issn:
  - 1613-6810
  - 1613-6829
publication_status: published
publisher: Wiley
status: public
title: Global Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures
  at the Single‐Nick Level
type: journal_article
user_id: '48864'
year: '2026'
...
---
_id: '60507'
abstract:
- lang: eng
  text: <jats:p>DNA origami nanostructures are powerful molecular tools for the controlled
    arrangement of functional molecules and thus have important applications in biomedicine,
    sensing, and materials science. The fabrication of DNA origami...</jats:p>
author:
- first_name: Emilia
  full_name: Tomm, Emilia
  id: '68157'
  last_name: Tomm
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
citation:
  ama: Tomm E, Grundmeier G, Keller A. Cost-efficient folding of functionalized DNA
    origami nanostructures via staple recycling. <i>Nanoscale</i>. Published online
    2025. doi:<a href="https://doi.org/10.1039/d5nr01435b">10.1039/d5nr01435b</a>
  apa: Tomm, E., Grundmeier, G., &#38; Keller, A. (2025). Cost-efficient folding of
    functionalized DNA origami nanostructures via staple recycling. <i>Nanoscale</i>.
    <a href="https://doi.org/10.1039/d5nr01435b">https://doi.org/10.1039/d5nr01435b</a>
  bibtex: '@article{Tomm_Grundmeier_Keller_2025, title={Cost-efficient folding of
    functionalized DNA origami nanostructures via staple recycling}, DOI={<a href="https://doi.org/10.1039/d5nr01435b">10.1039/d5nr01435b</a>},
    journal={Nanoscale}, publisher={Royal Society of Chemistry (RSC)}, author={Tomm,
    Emilia and Grundmeier, Guido and Keller, Adrian}, year={2025} }'
  chicago: Tomm, Emilia, Guido Grundmeier, and Adrian Keller. “Cost-Efficient Folding
    of Functionalized DNA Origami Nanostructures via Staple Recycling.” <i>Nanoscale</i>,
    2025. <a href="https://doi.org/10.1039/d5nr01435b">https://doi.org/10.1039/d5nr01435b</a>.
  ieee: 'E. Tomm, G. Grundmeier, and A. Keller, “Cost-efficient folding of functionalized
    DNA origami nanostructures via staple recycling,” <i>Nanoscale</i>, 2025, doi:
    <a href="https://doi.org/10.1039/d5nr01435b">10.1039/d5nr01435b</a>.'
  mla: Tomm, Emilia, et al. “Cost-Efficient Folding of Functionalized DNA Origami
    Nanostructures via Staple Recycling.” <i>Nanoscale</i>, Royal Society of Chemistry
    (RSC), 2025, doi:<a href="https://doi.org/10.1039/d5nr01435b">10.1039/d5nr01435b</a>.
  short: E. Tomm, G. Grundmeier, A. Keller, Nanoscale (2025).
date_created: 2025-07-03T11:26:30Z
date_updated: 2025-07-03T11:27:19Z
department:
- _id: '302'
doi: 10.1039/d5nr01435b
language:
- iso: eng
publication: Nanoscale
publication_identifier:
  issn:
  - 2040-3364
  - 2040-3372
publication_status: published
publisher: Royal Society of Chemistry (RSC)
status: public
title: Cost-efficient folding of functionalized DNA origami nanostructures via staple
  recycling
type: journal_article
user_id: '48864'
year: '2025'
...
---
_id: '60606'
abstract:
- lang: eng
  text: <jats:p>Streptavidin binding to DNA origami-supported high-density biotin
    arrays is investigated for selected experimental parameters. While bidentate binding
    and steric hindrance can be minimized, molecular crowding limits the binding yields
    in 2D arrays.</jats:p>
author:
- first_name: Lukas
  full_name: Rabbe, Lukas
  last_name: Rabbe
- first_name: Emilia
  full_name: Tomm, Emilia
  id: '68157'
  last_name: Tomm
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
citation:
  ama: Rabbe L, Tomm E, Grundmeier G, Keller A. Toward high-density streptavidin arrays
    on DNA origami nanostructures. <i>RSC Advances</i>. 2025;15(30):24536-24543. doi:<a
    href="https://doi.org/10.1039/d5ra03393d">10.1039/d5ra03393d</a>
  apa: Rabbe, L., Tomm, E., Grundmeier, G., &#38; Keller, A. (2025). Toward high-density
    streptavidin arrays on DNA origami nanostructures. <i>RSC Advances</i>, <i>15</i>(30),
    24536–24543. <a href="https://doi.org/10.1039/d5ra03393d">https://doi.org/10.1039/d5ra03393d</a>
  bibtex: '@article{Rabbe_Tomm_Grundmeier_Keller_2025, title={Toward high-density
    streptavidin arrays on DNA origami nanostructures}, volume={15}, DOI={<a href="https://doi.org/10.1039/d5ra03393d">10.1039/d5ra03393d</a>},
    number={30}, journal={RSC Advances}, publisher={Royal Society of Chemistry (RSC)},
    author={Rabbe, Lukas and Tomm, Emilia and Grundmeier, Guido and Keller, Adrian},
    year={2025}, pages={24536–24543} }'
  chicago: 'Rabbe, Lukas, Emilia Tomm, Guido Grundmeier, and Adrian Keller. “Toward
    High-Density Streptavidin Arrays on DNA Origami Nanostructures.” <i>RSC Advances</i>
    15, no. 30 (2025): 24536–43. <a href="https://doi.org/10.1039/d5ra03393d">https://doi.org/10.1039/d5ra03393d</a>.'
  ieee: 'L. Rabbe, E. Tomm, G. Grundmeier, and A. Keller, “Toward high-density streptavidin
    arrays on DNA origami nanostructures,” <i>RSC Advances</i>, vol. 15, no. 30, pp.
    24536–24543, 2025, doi: <a href="https://doi.org/10.1039/d5ra03393d">10.1039/d5ra03393d</a>.'
  mla: Rabbe, Lukas, et al. “Toward High-Density Streptavidin Arrays on DNA Origami
    Nanostructures.” <i>RSC Advances</i>, vol. 15, no. 30, Royal Society of Chemistry
    (RSC), 2025, pp. 24536–43, doi:<a href="https://doi.org/10.1039/d5ra03393d">10.1039/d5ra03393d</a>.
  short: L. Rabbe, E. Tomm, G. Grundmeier, A. Keller, RSC Advances 15 (2025) 24536–24543.
date_created: 2025-07-15T06:06:48Z
date_updated: 2025-07-15T06:07:16Z
department:
- _id: '302'
doi: 10.1039/d5ra03393d
intvolume: '        15'
issue: '30'
language:
- iso: eng
page: 24536-24543
publication: RSC Advances
publication_identifier:
  issn:
  - 2046-2069
publication_status: published
publisher: Royal Society of Chemistry (RSC)
status: public
title: Toward high-density streptavidin arrays on DNA origami nanostructures
type: journal_article
user_id: '48864'
volume: 15
year: '2025'
...
---
_id: '53621'
abstract:
- lang: eng
  text: <jats:p>The coupling of structural transitions to heat capacity changes leads
    to destabilization of macromolecules at both, elevated and lowered temperatures.
    DNA origami not only exhibit this property but also provide...</jats:p>
author:
- first_name: Daniel
  full_name: Dornbusch, Daniel
  last_name: Dornbusch
- first_name: Marcel
  full_name: Hanke, Marcel
  last_name: Hanke
- first_name: Emilia
  full_name: Tomm, Emilia
  id: '68157'
  last_name: Tomm
- first_name: Charlotte
  full_name: Kielar, Charlotte
  last_name: Kielar
- first_name: Guido
  full_name: Grundmeier, Guido
  id: '194'
  last_name: Grundmeier
- first_name: Adrian
  full_name: Keller, Adrian
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
- first_name: Karim
  full_name: Fahmy, Karim
  last_name: Fahmy
citation:
  ama: Dornbusch D, Hanke M, Tomm E, et al. Cold denaturation of DNA origami nanostructures.
    <i>Chemical Communications</i>. Published online 2024. doi:<a href="https://doi.org/10.1039/d3cc05985e">10.1039/d3cc05985e</a>
  apa: Dornbusch, D., Hanke, M., Tomm, E., Kielar, C., Grundmeier, G., Keller, A.,
    &#38; Fahmy, K. (2024). Cold denaturation of DNA origami nanostructures. <i>Chemical
    Communications</i>. <a href="https://doi.org/10.1039/d3cc05985e">https://doi.org/10.1039/d3cc05985e</a>
  bibtex: '@article{Dornbusch_Hanke_Tomm_Kielar_Grundmeier_Keller_Fahmy_2024, title={Cold
    denaturation of DNA origami nanostructures}, DOI={<a href="https://doi.org/10.1039/d3cc05985e">10.1039/d3cc05985e</a>},
    journal={Chemical Communications}, publisher={Royal Society of Chemistry (RSC)},
    author={Dornbusch, Daniel and Hanke, Marcel and Tomm, Emilia and Kielar, Charlotte
    and Grundmeier, Guido and Keller, Adrian and Fahmy, Karim}, year={2024} }'
  chicago: Dornbusch, Daniel, Marcel Hanke, Emilia Tomm, Charlotte Kielar, Guido Grundmeier,
    Adrian Keller, and Karim Fahmy. “Cold Denaturation of DNA Origami Nanostructures.”
    <i>Chemical Communications</i>, 2024. <a href="https://doi.org/10.1039/d3cc05985e">https://doi.org/10.1039/d3cc05985e</a>.
  ieee: 'D. Dornbusch <i>et al.</i>, “Cold denaturation of DNA origami nanostructures,”
    <i>Chemical Communications</i>, 2024, doi: <a href="https://doi.org/10.1039/d3cc05985e">10.1039/d3cc05985e</a>.'
  mla: Dornbusch, Daniel, et al. “Cold Denaturation of DNA Origami Nanostructures.”
    <i>Chemical Communications</i>, Royal Society of Chemistry (RSC), 2024, doi:<a
    href="https://doi.org/10.1039/d3cc05985e">10.1039/d3cc05985e</a>.
  short: D. Dornbusch, M. Hanke, E. Tomm, C. Kielar, G. Grundmeier, A. Keller, K.
    Fahmy, Chemical Communications (2024).
date_created: 2024-04-23T08:20:05Z
date_updated: 2024-04-23T08:21:05Z
department:
- _id: '302'
doi: 10.1039/d3cc05985e
keyword:
- Materials Chemistry
- Metals and Alloys
- Surfaces
- Coatings and Films
- General Chemistry
- Ceramics and Composites
- Electronic
- Optical and Magnetic Materials
- Catalysis
language:
- iso: eng
publication: Chemical Communications
publication_identifier:
  issn:
  - 1359-7345
  - 1364-548X
publication_status: published
publisher: Royal Society of Chemistry (RSC)
status: public
title: Cold denaturation of DNA origami nanostructures
type: journal_article
user_id: '48864'
year: '2024'
...