---
res:
bibo_abstract:
- "Abstract\r\n Doxorubicin (DOX)
is a common drug in cancer chemotherapy, and its high DNA-binding affinity can
be harnessed in preparing DOX-loaded DNA nanostructures for targeted delivery
and therapeutics. Although DOX has been widely studied, the existing literature
of DOX-loaded DNA-carriers remains limited and incoherent. Here, based on an in-depth
spectroscopic analysis, we characterize and optimize the DOX loading into different
2D and 3D scaffolded DNA origami nanostructures (DONs). In our experimental conditions,
all DONs show similar DOX binding capacities (one DOX molecule per two to three
base pairs), and the binding equilibrium is reached within seconds, remarkably
faster than previously acknowledged. To characterize drug release profiles, DON
degradation and DOX release from the complexes upon DNase I digestion was studied.
For the employed DONs, the relative doses (DOX molecules released per unit time)
may vary by two orders of magnitude depending on the DON superstructure. In addition,
we identify DOX aggregation mechanisms and spectral changes linked to pH, magnesium,
and DOX concentration. These features have been largely ignored in experimenting
with DNA nanostructures, but are probably the major sources of the incoherence
of the experimental results so far. Therefore, we believe this work can act as
a guide to tailoring the release profiles and developing better drug delivery
systems based on DNA-carriers.@eng"
bibo_authorlist:
- foaf_Person:
foaf_givenName: Heini
foaf_name: Ijäs, Heini
foaf_surname: Ijäs
- foaf_Person:
foaf_givenName: Boxuan
foaf_name: Shen, Boxuan
foaf_surname: Shen
- foaf_Person:
foaf_givenName: Amelie
foaf_name: Heuer-Jungemann, Amelie
foaf_surname: Heuer-Jungemann
- foaf_Person:
foaf_givenName: Adrian
foaf_name: Keller, Adrian
foaf_surname: Keller
foaf_workInfoHomepage: http://www.librecat.org/personId=48864
orcid: 0000-0001-7139-3110
- foaf_Person:
foaf_givenName: Mauri A
foaf_name: Kostiainen, Mauri A
foaf_surname: Kostiainen
- foaf_Person:
foaf_givenName: Tim
foaf_name: Liedl, Tim
foaf_surname: Liedl
- foaf_Person:
foaf_givenName: Janne A
foaf_name: Ihalainen, Janne A
foaf_surname: Ihalainen
- foaf_Person:
foaf_givenName: Veikko
foaf_name: Linko, Veikko
foaf_surname: Linko
bibo_doi: 10.1093/nar/gkab097
bibo_volume: 49
dct_date: 2021^xs_gYear
dct_isPartOf:
- http://id.crossref.org/issn/0305-1048
- http://id.crossref.org/issn/1362-4962
dct_language: eng
dct_title: Unraveling the interaction between doxorubicin and DNA origami nanostructures
for customizable chemotherapeutic drug release@
...