TY - JOUR AB - AbstractAnorexia nervosa (AN) is a severe eating disorder and often associated with altered humoral immune responses. However, distinct B cell maturation stages in peripheral blood in adolescents with AN have not been characterized. Treatment effects and the relationship between clinical and B cell parameters are also not fully understood. Here we investigated the phenotype of circulating B cell subsets and the relationship with body composition in adolescents with AN before (T0, n = 24) and after 6 weeks (T1, n = 20) of treatment. Using multi-parameter flow cytometry, we found increased percentages of antigen-experienced B cells and plasmablasts in patients with AN compared to healthy controls (n = 20). In contrast, percentages of CD1d+CD5+ B cells and transitional B cells with immunoregulatory roles were reduced at T0 and T1. These B cell frequencies correlated positively with fat mass, fat mass index (FMI), free fat mass index, and body mass index standard deviation score. In addition, scavenger-like receptor CD5 expression levels were downregulated on transitional B cells and correlated with fat mass and FMI in AN. Our findings that regulatory B cell subgroups were reduced in AN and their strong relationship with body composition parameters point toward an impact of immunoregulatory B cells in the pathogenesis of AN. AU - Freff, Jana AU - Schwarte, Kathrin AU - Bröker, Lisa AU - Bühlmeier, Judith AU - Kraft, Isabelle AU - Öztürk, Dana AU - Hinney, Anke AU - Arolt, Volker AU - Dannlowski, Udo AU - Romer, Georg AU - Baune, Bernhard T. AU - Hebebrand, Johannes AU - Föcker, Manuel AU - Alferink, Judith ID - 26632 JF - Scientific Reports SN - 2045-2322 TI - Alterations in B cell subsets correlate with body composition parameters in female adolescents with anorexia nervosa ER -