{"publication_status":"published","issue":"5","doi":"10.3390/ijms23052817","page":"2817","abstract":[{"lang":"eng","text":"<jats:p>DNA origami technology enables the folding of DNA strands into complex nanoscale shapes whose properties and interactions with molecular species often deviate significantly from that of genomic DNA. Here, we investigate the salting-out of different DNA origami shapes by the kosmotropic salt ammonium sulfate that is routinely employed in protein precipitation. We find that centrifugation in the presence of 3 M ammonium sulfate results in notable precipitation of DNA origami nanostructures but not of double-stranded genomic DNA. The precipitated DNA origami nanostructures can be resuspended in ammonium sulfate-free buffer without apparent formation of aggregates or loss of structural integrity. Even though quasi-1D six-helix bundle DNA origami are slightly less susceptible toward salting-out than more compact DNA origami triangles and 24-helix bundles, precipitation and recovery yields appear to be mostly independent of DNA origami shape and superstructure. Exploiting the specificity of ammonium sulfate salting-out for DNA origami nanostructures, we further apply this method to separate DNA origami triangles from genomic DNA fragments in a complex mixture. Our results thus demonstrate the possibility of concentrating and purifying DNA origami nanostructures by ammonium sulfate-induced salting-out.</jats:p>"}],"keyword":["Inorganic Chemistry","Organic Chemistry","Physical and Theoretical Chemistry","Computer Science Applications","Spectroscopy","Molecular Biology","General Medicine","Catalysis"],"date_created":"2022-03-07T07:28:02Z","language":[{"iso":"eng"}],"date_updated":"2022-03-07T07:29:27Z","type":"journal_article","publication":"International Journal of Molecular Sciences","status":"public","intvolume":"        23","year":"2022","author":[{"full_name":"Hanke, Marcel","last_name":"Hanke","first_name":"Marcel"},{"first_name":"Niklas","full_name":"Hansen, Niklas","last_name":"Hansen"},{"first_name":"Ruiping","full_name":"Chen, Ruiping","last_name":"Chen"},{"first_name":"Guido","last_name":"Grundmeier","full_name":"Grundmeier, Guido"},{"first_name":"Karim","full_name":"Fahmy, Karim","last_name":"Fahmy"},{"first_name":"Adrian","last_name":"Keller","full_name":"Keller, Adrian"}],"publisher":"MDPI AG","_id":"30209","title":"Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate","publication_identifier":{"issn":["1422-0067"]},"department":[{"_id":"302"}],"volume":23,"user_id":"48864","citation":{"chicago":"Hanke, Marcel, Niklas Hansen, Ruiping Chen, Guido Grundmeier, Karim Fahmy, and Adrian Keller. “Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate.” <i>International Journal of Molecular Sciences</i> 23, no. 5 (2022): 2817. <a href=\"https://doi.org/10.3390/ijms23052817\">https://doi.org/10.3390/ijms23052817</a>.","short":"M. Hanke, N. Hansen, R. Chen, G. Grundmeier, K. Fahmy, A. Keller, International Journal of Molecular Sciences 23 (2022) 2817.","apa":"Hanke, M., Hansen, N., Chen, R., Grundmeier, G., Fahmy, K., &#38; Keller, A. (2022). Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate. <i>International Journal of Molecular Sciences</i>, <i>23</i>(5), 2817. <a href=\"https://doi.org/10.3390/ijms23052817\">https://doi.org/10.3390/ijms23052817</a>","mla":"Hanke, Marcel, et al. “Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate.” <i>International Journal of Molecular Sciences</i>, vol. 23, no. 5, MDPI AG, 2022, p. 2817, doi:<a href=\"https://doi.org/10.3390/ijms23052817\">10.3390/ijms23052817</a>.","bibtex":"@article{Hanke_Hansen_Chen_Grundmeier_Fahmy_Keller_2022, title={Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate}, volume={23}, DOI={<a href=\"https://doi.org/10.3390/ijms23052817\">10.3390/ijms23052817</a>}, number={5}, journal={International Journal of Molecular Sciences}, publisher={MDPI AG}, author={Hanke, Marcel and Hansen, Niklas and Chen, Ruiping and Grundmeier, Guido and Fahmy, Karim and Keller, Adrian}, year={2022}, pages={2817} }","ama":"Hanke M, Hansen N, Chen R, Grundmeier G, Fahmy K, Keller A. Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate. <i>International Journal of Molecular Sciences</i>. 2022;23(5):2817. doi:<a href=\"https://doi.org/10.3390/ijms23052817\">10.3390/ijms23052817</a>","ieee":"M. Hanke, N. Hansen, R. Chen, G. Grundmeier, K. Fahmy, and A. Keller, “Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate,” <i>International Journal of Molecular Sciences</i>, vol. 23, no. 5, p. 2817, 2022, doi: <a href=\"https://doi.org/10.3390/ijms23052817\">10.3390/ijms23052817</a>."}}