---
_id: '30209'
abstract:
- lang: eng
  text: <jats:p>DNA origami technology enables the folding of DNA strands into complex
    nanoscale shapes whose properties and interactions with molecular species often
    deviate significantly from that of genomic DNA. Here, we investigate the salting-out
    of different DNA origami shapes by the kosmotropic salt ammonium sulfate that
    is routinely employed in protein precipitation. We find that centrifugation in
    the presence of 3 M ammonium sulfate results in notable precipitation of DNA origami
    nanostructures but not of double-stranded genomic DNA. The precipitated DNA origami
    nanostructures can be resuspended in ammonium sulfate-free buffer without apparent
    formation of aggregates or loss of structural integrity. Even though quasi-1D
    six-helix bundle DNA origami are slightly less susceptible toward salting-out
    than more compact DNA origami triangles and 24-helix bundles, precipitation and
    recovery yields appear to be mostly independent of DNA origami shape and superstructure.
    Exploiting the specificity of ammonium sulfate salting-out for DNA origami nanostructures,
    we further apply this method to separate DNA origami triangles from genomic DNA
    fragments in a complex mixture. Our results thus demonstrate the possibility of
    concentrating and purifying DNA origami nanostructures by ammonium sulfate-induced
    salting-out.</jats:p>
author:
- first_name: Marcel
  full_name: Hanke, Marcel
  last_name: Hanke
- first_name: Niklas
  full_name: Hansen, Niklas
  last_name: Hansen
- first_name: Ruiping
  full_name: Chen, Ruiping
  last_name: Chen
- first_name: Guido
  full_name: Grundmeier, Guido
  last_name: Grundmeier
- first_name: Karim
  full_name: Fahmy, Karim
  last_name: Fahmy
- first_name: Adrian
  full_name: Keller, Adrian
  last_name: Keller
citation:
  ama: Hanke M, Hansen N, Chen R, Grundmeier G, Fahmy K, Keller A. Salting-Out of
    DNA Origami Nanostructures by Ammonium Sulfate. <i>International Journal of Molecular
    Sciences</i>. 2022;23(5):2817. doi:<a href="https://doi.org/10.3390/ijms23052817">10.3390/ijms23052817</a>
  apa: Hanke, M., Hansen, N., Chen, R., Grundmeier, G., Fahmy, K., &#38; Keller, A.
    (2022). Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate. <i>International
    Journal of Molecular Sciences</i>, <i>23</i>(5), 2817. <a href="https://doi.org/10.3390/ijms23052817">https://doi.org/10.3390/ijms23052817</a>
  bibtex: '@article{Hanke_Hansen_Chen_Grundmeier_Fahmy_Keller_2022, title={Salting-Out
    of DNA Origami Nanostructures by Ammonium Sulfate}, volume={23}, DOI={<a href="https://doi.org/10.3390/ijms23052817">10.3390/ijms23052817</a>},
    number={5}, journal={International Journal of Molecular Sciences}, publisher={MDPI
    AG}, author={Hanke, Marcel and Hansen, Niklas and Chen, Ruiping and Grundmeier,
    Guido and Fahmy, Karim and Keller, Adrian}, year={2022}, pages={2817} }'
  chicago: 'Hanke, Marcel, Niklas Hansen, Ruiping Chen, Guido Grundmeier, Karim Fahmy,
    and Adrian Keller. “Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate.”
    <i>International Journal of Molecular Sciences</i> 23, no. 5 (2022): 2817. <a
    href="https://doi.org/10.3390/ijms23052817">https://doi.org/10.3390/ijms23052817</a>.'
  ieee: 'M. Hanke, N. Hansen, R. Chen, G. Grundmeier, K. Fahmy, and A. Keller, “Salting-Out
    of DNA Origami Nanostructures by Ammonium Sulfate,” <i>International Journal of
    Molecular Sciences</i>, vol. 23, no. 5, p. 2817, 2022, doi: <a href="https://doi.org/10.3390/ijms23052817">10.3390/ijms23052817</a>.'
  mla: Hanke, Marcel, et al. “Salting-Out of DNA Origami Nanostructures by Ammonium
    Sulfate.” <i>International Journal of Molecular Sciences</i>, vol. 23, no. 5,
    MDPI AG, 2022, p. 2817, doi:<a href="https://doi.org/10.3390/ijms23052817">10.3390/ijms23052817</a>.
  short: M. Hanke, N. Hansen, R. Chen, G. Grundmeier, K. Fahmy, A. Keller, International
    Journal of Molecular Sciences 23 (2022) 2817.
date_created: 2022-03-07T07:28:02Z
date_updated: 2022-03-07T07:29:27Z
department:
- _id: '302'
doi: 10.3390/ijms23052817
intvolume: '        23'
issue: '5'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Computer Science Applications
- Spectroscopy
- Molecular Biology
- General Medicine
- Catalysis
language:
- iso: eng
page: '2817'
publication: International Journal of Molecular Sciences
publication_identifier:
  issn:
  - 1422-0067
publication_status: published
publisher: MDPI AG
status: public
title: Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate
type: journal_article
user_id: '48864'
volume: 23
year: '2022'
...