{"status":"public","doi":"10.1159/000257516","title":"Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1","volume":24,"year":"2009","type":"journal_article","_id":"54938","author":[{"last_name":"Krueger","orcid":"0000-0001-5351-1785","full_name":"Krueger, Bettina","first_name":"Bettina","id":"49428"},{"last_name":"Haerteis","full_name":"Haerteis, Silke","first_name":"Silke"},{"last_name":"Yang","first_name":"Limin","full_name":"Yang, Limin"},{"last_name":"Hartner","first_name":"Andrea","full_name":"Hartner, Andrea"},{"last_name":"Rauh","full_name":"Rauh, Robert","first_name":"Robert"},{"last_name":"Korbmacher","first_name":"Christoph","full_name":"Korbmacher, Christoph"},{"full_name":"Diakov, Alexei","first_name":"Alexei","last_name":"Diakov"}],"date_created":"2024-06-30T13:52:19Z","department":[{"_id":"35"},{"_id":"22"}],"date_updated":"2024-06-30T13:52:27Z","publisher":"S. Karger AG","issue":"5-6","language":[{"iso":"eng"}],"intvolume":"        24","publication":"Cellular Physiology and Biochemistry","citation":{"bibtex":"@article{Krueger_Haerteis_Yang_Hartner_Rauh_Korbmacher_Diakov_2009, title={Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1}, volume={24}, DOI={<a href=\"https://doi.org/10.1159/000257516\">10.1159/000257516</a>}, number={5–6}, journal={Cellular Physiology and Biochemistry}, publisher={S. Karger AG}, author={Krueger, Bettina and Haerteis, Silke and Yang, Limin and Hartner, Andrea and Rauh, Robert and Korbmacher, Christoph and Diakov, Alexei}, year={2009}, pages={605–618} }","mla":"Krueger, Bettina, et al. “Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1.” <i>Cellular Physiology and Biochemistry</i>, vol. 24, no. 5–6, S. Karger AG, 2009, pp. 605–618, doi:<a href=\"https://doi.org/10.1159/000257516\">10.1159/000257516</a>.","ama":"Krueger B, Haerteis S, Yang L, et al. Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1. <i>Cellular Physiology and Biochemistry</i>. 2009;24(5-6):605–618. doi:<a href=\"https://doi.org/10.1159/000257516\">10.1159/000257516</a>","short":"B. Krueger, S. Haerteis, L. Yang, A. Hartner, R. Rauh, C. Korbmacher, A. Diakov, Cellular Physiology and Biochemistry 24 (2009) 605–618.","ieee":"B. Krueger <i>et al.</i>, “Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1,” <i>Cellular Physiology and Biochemistry</i>, vol. 24, no. 5–6, pp. 605–618, 2009, doi: <a href=\"https://doi.org/10.1159/000257516\">10.1159/000257516</a>.","chicago":"Krueger, Bettina, Silke Haerteis, Limin Yang, Andrea Hartner, Robert Rauh, Christoph Korbmacher, and Alexei Diakov. “Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1.” <i>Cellular Physiology and Biochemistry</i> 24, no. 5–6 (2009): 605–618. <a href=\"https://doi.org/10.1159/000257516\">https://doi.org/10.1159/000257516</a>.","apa":"Krueger, B., Haerteis, S., Yang, L., Hartner, A., Rauh, R., Korbmacher, C., &#38; Diakov, A. (2009). Cholesterol Depletion of the Plasma Membrane Prevents Activation of the Epithelial Sodium Channel (ENaC) by SGK1. <i>Cellular Physiology and Biochemistry</i>, <i>24</i>(5–6), 605–618. <a href=\"https://doi.org/10.1159/000257516\">https://doi.org/10.1159/000257516</a>"},"page":"605–618","user_id":"49428","abstract":[{"lang":"eng","text":"The lipid environment of the epithelial sodium channel (ENaC) and its possible association with so-called lipid rafts may be relevant to its function. The aim of our study was to confirm the association of ENaC with lipid rafts and to analyze the effect of cholesterol depletion of the plasma membrane by methyl-beta-cyclodextrin (MbetaCD) on channel function and regulation. Using sucrose density gradient centrifugation we demonstrated that a significant portion of ENaC protein distributes to low density fractions thought to be typical lipid raft fractions. Importantly, cholesterol depletion of cell lysate by MbetaCD shifted ENaC to non-raft fractions of higher density. Live cell imaging demonstrated that treatment with MbetaCD largely reduced filipin staining over time, confirming cholesterol depletion of the plasma membrane. For electrophysiological studies intact oocytes were exposed to 20 mM MbetaCD for three hours. MbetaCD treatment had no consistent effect on baseline whole-cell ENaC currents. In addition to the typical single channel conductance of about 5 pS, subconductance states of ENaC were occasionally observed in patches from MbetaCD treated but not from control oocytes. Importantly, in outside-out patch clamp recordings the stimulatory effect of recombinant SGK1 in the pipette solution was essentially abolished in oocytes pretreated with MbetaCD. These results indicate that ENaC activation by cytosolic SGK1 is compromised by removing cholesterol from the plasma membrane. Thus, ENaC activation by SGK1 may require the presence of an intact lipid environment and/or of lipid rafts as signalling platform."}]}