{"date_created":"2025-12-15T09:54:41Z","type":"journal_article","volume":338,"department":[{"_id":"35"},{"_id":"2"},{"_id":"307"}],"status":"public","author":[{"first_name":"Benjamin W.","full_name":"Moorlach, Benjamin W.","last_name":"Moorlach"},{"first_name":"Robert","full_name":"Epkenhans, Robert","last_name":"Epkenhans"},{"last_name":"Ju","full_name":"Ju, Di","first_name":"Di"},{"last_name":"Ravidas","full_name":"Ravidas, Banuja","first_name":"Banuja"},{"last_name":"Weinberger","full_name":"Weinberger, Christian","id":"11848","first_name":"Christian"},{"last_name":"Tiemann","orcid":"0000-0003-1711-2722","full_name":"Tiemann, Michael","first_name":"Michael","id":"23547"},{"first_name":"Judith","last_name":"Buente","full_name":"Buente, Judith"},{"full_name":"Gaerner, Maik","last_name":"Gaerner","first_name":"Maik"},{"first_name":"Martin","full_name":"Wortmann, Martin","last_name":"Wortmann"},{"full_name":"Scholten, Stefan","last_name":"Scholten","first_name":"Stefan"},{"last_name":"Rostas","full_name":"Rostas, Michael","first_name":"Michael"},{"first_name":"Waldemar","last_name":"Keil","full_name":"Keil, Waldemar"},{"last_name":"Patel","full_name":"Patel, Anant V.","first_name":"Anant V."}],"oa":"1","language":[{"iso":"eng"}],"doi":"10.1016/j.ijbiomac.2025.149697","main_file_link":[{"open_access":"1"}],"publication":"International Journal of Biological Macromolecules","_id":"63099","citation":{"ieee":"B. W. Moorlach et al., “DsRNA-based carriers with pH-tuneable release kinetics for effective control of Psylliodes chrysocephala,” International Journal of Biological Macromolecules, vol. 338, Art. no. 149697, 2026, doi: 10.1016/j.ijbiomac.2025.149697.","mla":"Moorlach, Benjamin W., et al. “DsRNA-Based Carriers with PH-Tuneable Release Kinetics for Effective Control of Psylliodes Chrysocephala.” International Journal of Biological Macromolecules, vol. 338, 149697, Elsevier BV, 2026, doi:10.1016/j.ijbiomac.2025.149697.","bibtex":"@article{Moorlach_Epkenhans_Ju_Ravidas_Weinberger_Tiemann_Buente_Gaerner_Wortmann_Scholten_et al._2026, title={DsRNA-based carriers with pH-tuneable release kinetics for effective control of Psylliodes chrysocephala}, volume={338}, DOI={10.1016/j.ijbiomac.2025.149697}, number={149697}, journal={International Journal of Biological Macromolecules}, publisher={Elsevier BV}, author={Moorlach, Benjamin W. and Epkenhans, Robert and Ju, Di and Ravidas, Banuja and Weinberger, Christian and Tiemann, Michael and Buente, Judith and Gaerner, Maik and Wortmann, Martin and Scholten, Stefan and et al.}, year={2026} }","ama":"Moorlach BW, Epkenhans R, Ju D, et al. DsRNA-based carriers with pH-tuneable release kinetics for effective control of Psylliodes chrysocephala. International Journal of Biological Macromolecules. 2026;338. doi:10.1016/j.ijbiomac.2025.149697","short":"B.W. Moorlach, R. Epkenhans, D. Ju, B. Ravidas, C. Weinberger, M. Tiemann, J. Buente, M. Gaerner, M. Wortmann, S. Scholten, M. Rostas, W. Keil, A.V. Patel, International Journal of Biological Macromolecules 338 (2026).","apa":"Moorlach, B. W., Epkenhans, R., Ju, D., Ravidas, B., Weinberger, C., Tiemann, M., Buente, J., Gaerner, M., Wortmann, M., Scholten, S., Rostas, M., Keil, W., & Patel, A. V. (2026). DsRNA-based carriers with pH-tuneable release kinetics for effective control of Psylliodes chrysocephala. International Journal of Biological Macromolecules, 338, Article 149697. https://doi.org/10.1016/j.ijbiomac.2025.149697","chicago":"Moorlach, Benjamin W., Robert Epkenhans, Di Ju, Banuja Ravidas, Christian Weinberger, Michael Tiemann, Judith Buente, et al. “DsRNA-Based Carriers with PH-Tuneable Release Kinetics for Effective Control of Psylliodes Chrysocephala.” International Journal of Biological Macromolecules 338 (2026). https://doi.org/10.1016/j.ijbiomac.2025.149697."},"year":"2026","date_updated":"2025-12-17T07:27:57Z","intvolume":" 338","publication_identifier":{"issn":["0141-8130"]},"article_type":"original","abstract":[{"lang":"eng","text":"Spray-induced gene silencing (SIGS) employing double-stranded RNA (dsRNA) offers a promising, species-specific approach for protecting crops from insect pests such as the cabbage stem flea beetle (Psylliodes chrysocephala). However, the environmental instability of dsRNA presents a major limitation to its field application. In this study, we evaluate two distinct dsRNA formulation strategies for improved stability and delivery: a bottom-up approach using chitosan-based interpolyelectrolyte complexes (IPEC) and a top-down approach employing functionalized mesoporous silica carriers (SBA-15). Both systems were comprehensively characterized in terms of size, surface potential, porosity, and release behavior. The results revealed that IPECs exhibited release kinetics that were approximately one order of magnitude faster than those of SBA-15 across all tested conditions. The two formulations significantly improved dsRNA stability against UV and heat exposure compared to free dsRNA. In feeding assays with P. chrysocephala, both carriers achieved comparable gene silencing efficacy, though dsRNA@IPEC induced more immediate effects, while dsRNA@SBA-15 displayed delayed but ultimately stronger reduction in consumed leaf area, consistent with its slower release kinetics. We demonstrate that despite structural and mechanistic differences, both delivery platforms effectively stabilized and delivered dsRNA, and offered distinct advantages depending on application needs. This work highlights how formulation strategies are key to successful SIGS and supports the development of robust, field-adaptable formulation technologies for sustainable pest management."}],"quality_controlled":"1","publication_status":"published","article_number":"149697","publisher":"Elsevier BV","user_id":"23547","title":"DsRNA-based carriers with pH-tuneable release kinetics for effective control of Psylliodes chrysocephala"}