---
res:
  bibo_abstract:
  - "<jats:title>ABSTRACT</jats:title>\r\n                  <jats:p>Ligation of staple
    strands in DNA origami nanostructures (DONs) can yield enhanced structural stability
    in critical environments. This process can be viewed as performing hundreds of
    parallel reactions programmed on a self‐assembled nanoscale platform. While previous
    studies have focused on investigating the collective results of the chemical or
    enzymatic ligation reactions, herein, the global quantitative analysis of individual
    ligation reactions is achieved using quantitative PCR (qPCR). By mapping enzymatic
    ligation efficiency on a trapezoidal substructure representing one‐third of a
    triangular DON, ligation is shown to preferentially occur at the trapezoid edges
    rather than at inner sites. Excellent agreement between the experimental ligation
    yields and docking simulations suggests that this is a result of variations in
    the ligase docking probability. Ligation products involving more than two consecutive
    sequences can be generated with each enzyme‐catalyzed reaction as an independent
    event. Interestingly, the sharp contrast between the edges vs. the inner sites
    has been abolished by changing the reaction conditions and performing the ligation
    in a DMSO co‐solvent system. This analytic method provides unprecedented insight
    into the multiple ligation reactions occurring in parallel within complex DONs
    and will be an invaluable tool in the translation of DONs from the lab to real‐world
    applications.</jats:p>@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Konrad
      foaf_name: Hacker, Konrad
      foaf_surname: Hacker
  - foaf_Person:
      foaf_givenName: Emilia
      foaf_name: Juricke, Emilia
      foaf_surname: Juricke
      foaf_workInfoHomepage: http://www.librecat.org/personId=68157
  - foaf_Person:
      foaf_givenName: Carolin
      foaf_name: Münch, Carolin
      foaf_surname: Münch
  - foaf_Person:
      foaf_givenName: Antonio
      foaf_name: Suma, Antonio
      foaf_surname: Suma
  - foaf_Person:
      foaf_givenName: Adrian Clemens
      foaf_name: Keller, Adrian Clemens
      foaf_surname: Keller
      foaf_workInfoHomepage: http://www.librecat.org/personId=48864
    orcid: 0000-0001-7139-3110
  - foaf_Person:
      foaf_givenName: Yixin
      foaf_name: Zhang, Yixin
      foaf_surname: Zhang
  bibo_doi: 10.1002/smll.202508136
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1613-6810
  - http://id.crossref.org/issn/1613-6829
  dct_language: eng
  dct_publisher: Wiley@
  dct_title: Global Quantitative Analysis of Ligation Reactions in Self‐Assembled
    DNA Nanostructures at the Single‐Nick Level@
...