---
_id: '65545'
abstract:
- lang: eng
  text: "<jats:title>ABSTRACT</jats:title>\r\n                  <jats:p>Ligation of
    staple strands in DNA origami nanostructures (DONs) can yield enhanced structural
    stability in critical environments. This process can be viewed as performing hundreds
    of parallel reactions programmed on a self‐assembled nanoscale platform. While
    previous studies have focused on investigating the collective results of the chemical
    or enzymatic ligation reactions, herein, the global quantitative analysis of individual
    ligation reactions is achieved using quantitative PCR (qPCR). By mapping enzymatic
    ligation efficiency on a trapezoidal substructure representing one‐third of a
    triangular DON, ligation is shown to preferentially occur at the trapezoid edges
    rather than at inner sites. Excellent agreement between the experimental ligation
    yields and docking simulations suggests that this is a result of variations in
    the ligase docking probability. Ligation products involving more than two consecutive
    sequences can be generated with each enzyme‐catalyzed reaction as an independent
    event. Interestingly, the sharp contrast between the edges vs. the inner sites
    has been abolished by changing the reaction conditions and performing the ligation
    in a DMSO co‐solvent system. This analytic method provides unprecedented insight
    into the multiple ligation reactions occurring in parallel within complex DONs
    and will be an invaluable tool in the translation of DONs from the lab to real‐world
    applications.</jats:p>"
article_number: e08136
author:
- first_name: Konrad
  full_name: Hacker, Konrad
  last_name: Hacker
- first_name: Emilia
  full_name: Juricke, Emilia
  id: '68157'
  last_name: Juricke
- first_name: Carolin
  full_name: Münch, Carolin
  last_name: Münch
- first_name: Antonio
  full_name: Suma, Antonio
  last_name: Suma
- first_name: Adrian Clemens
  full_name: Keller, Adrian Clemens
  id: '48864'
  last_name: Keller
  orcid: 0000-0001-7139-3110
- first_name: Yixin
  full_name: Zhang, Yixin
  last_name: Zhang
citation:
  ama: Hacker K, Juricke E, Münch C, Suma A, Keller AC, Zhang Y. Global Quantitative
    Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures at the Single‐Nick
    Level. <i>Small</i>. Published online 2026. doi:<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>
  apa: Hacker, K., Juricke, E., Münch, C., Suma, A., Keller, A. C., &#38; Zhang, Y.
    (2026). Global Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA
    Nanostructures at the Single‐Nick Level. <i>Small</i>, Article e08136. <a href="https://doi.org/10.1002/smll.202508136">https://doi.org/10.1002/smll.202508136</a>
  bibtex: '@article{Hacker_Juricke_Münch_Suma_Keller_Zhang_2026, title={Global Quantitative
    Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures at the Single‐Nick
    Level}, DOI={<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>},
    number={e08136}, journal={Small}, publisher={Wiley}, author={Hacker, Konrad and
    Juricke, Emilia and Münch, Carolin and Suma, Antonio and Keller, Adrian Clemens
    and Zhang, Yixin}, year={2026} }'
  chicago: Hacker, Konrad, Emilia Juricke, Carolin Münch, Antonio Suma, Adrian Clemens
    Keller, and Yixin Zhang. “Global Quantitative Analysis of Ligation Reactions in
    Self‐Assembled DNA Nanostructures at the Single‐Nick Level.” <i>Small</i>, 2026.
    <a href="https://doi.org/10.1002/smll.202508136">https://doi.org/10.1002/smll.202508136</a>.
  ieee: 'K. Hacker, E. Juricke, C. Münch, A. Suma, A. C. Keller, and Y. Zhang, “Global
    Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures
    at the Single‐Nick Level,” <i>Small</i>, Art. no. e08136, 2026, doi: <a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>.'
  mla: Hacker, Konrad, et al. “Global Quantitative Analysis of Ligation Reactions
    in Self‐Assembled DNA Nanostructures at the Single‐Nick Level.” <i>Small</i>,
    e08136, Wiley, 2026, doi:<a href="https://doi.org/10.1002/smll.202508136">10.1002/smll.202508136</a>.
  short: K. Hacker, E. Juricke, C. Münch, A. Suma, A.C. Keller, Y. Zhang, Small (2026).
date_created: 2026-05-02T10:20:10Z
date_updated: 2026-05-02T10:20:35Z
department:
- _id: '302'
doi: 10.1002/smll.202508136
language:
- iso: eng
publication: Small
publication_identifier:
  issn:
  - 1613-6810
  - 1613-6829
publication_status: published
publisher: Wiley
status: public
title: Global Quantitative Analysis of Ligation Reactions in Self‐Assembled DNA Nanostructures
  at the Single‐Nick Level
type: journal_article
user_id: '48864'
year: '2026'
...