article published Özge Coşkuner Leineweber author Ulrike Hofmann author Guido Grundmeier author 194 Yixin Zhang author Adrian Clemens Keller author 488640000-0001-7139-3110 302 department <jats:p> DNA origami nanostructures (DONs) have promising applications in biomedicine and biosensing, which often require their efficient binding to target cells. By immobilizing the glycopeptide antibiotic vancomycin on DONs, DON binding to Gram‐positive and Gram‐negative bacteria can be facilitated. Here, we investigate how this multivalent binding is affected by the number and arrangement of the vancomycin modifications on two‐dimensional DONs. We find that for both Gram‐positive <jats:italic>Bacillus subtilis</jats:italic> and Gram‐negative <jats:italic>Escherichia coli</jats:italic> , binding increases with the number of vancomycin modifications per DON. In general, binding to <jats:italic>E. coli</jats:italic> is stronger than to <jats:italic>B. subtilis</jats:italic> , which may be attributed to differences in the architectures of the cell envelopes. Interestingly, for both bacteria, the total number of vancomycin modifications appears to be more important than their arrangement, as DONs with 18 vancomycin molecules on one side show similar binding as DONs with 18 vancomycin molecules distributed over both sides. This enables the attachment of multiple probe molecules to the vancomycin‐free side of the DONs for enhancing detection efficiency without compromising binding affinity. These results may thus provide guidelines for the design and synthesis of vancomycin‐modified DONs for antimicrobial drug delivery and pathogen detection. </jats:p> Wiley2026 eng 1439-4227 1439-763310.1002/cbic.70436 2713 Coşkuner Leineweber, Özge, Ulrike Hofmann, Guido Grundmeier, Yixin Zhang, and Adrian Clemens Keller. “Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria.” <i>ChemBioChem</i> 27, no. 13 (2026). <a href="https://doi.org/10.1002/cbic.70436">https://doi.org/10.1002/cbic.70436</a>. Ö. Coşkuner Leineweber, U. Hofmann, G. Grundmeier, Y. Zhang, A.C. Keller, ChemBioChem 27 (2026). Ö. Coşkuner Leineweber, U. Hofmann, G. Grundmeier, Y. Zhang, and A. C. Keller, “Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria,” <i>ChemBioChem</i>, vol. 27, no. 13, Art. no. e70436, 2026, doi: <a href="https://doi.org/10.1002/cbic.70436">10.1002/cbic.70436</a>. Coşkuner Leineweber, Ö., Hofmann, U., Grundmeier, G., Zhang, Y., &#38; Keller, A. C. (2026). Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria. <i>ChemBioChem</i>, <i>27</i>(13), Article e70436. <a href="https://doi.org/10.1002/cbic.70436">https://doi.org/10.1002/cbic.70436</a> @article{Coşkuner Leineweber_Hofmann_Grundmeier_Zhang_Keller_2026, title={Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria}, volume={27}, DOI={<a href="https://doi.org/10.1002/cbic.70436">10.1002/cbic.70436</a>}, number={13e70436}, journal={ChemBioChem}, publisher={Wiley}, author={Coşkuner Leineweber, Özge and Hofmann, Ulrike and Grundmeier, Guido and Zhang, Yixin and Keller, Adrian Clemens}, year={2026} } Coşkuner Leineweber Ö, Hofmann U, Grundmeier G, Zhang Y, Keller AC. Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria. <i>ChemBioChem</i>. 2026;27(13). doi:<a href="https://doi.org/10.1002/cbic.70436">10.1002/cbic.70436</a> Coşkuner Leineweber, Özge, et al. “Vancomycin‐Mediated Binding of DNA Origami Nanostructures to Gram‐Positive and Gram‐Negative Bacteria.” <i>ChemBioChem</i>, vol. 27, no. 13, e70436, Wiley, 2026, doi:<a href="https://doi.org/10.1002/cbic.70436">10.1002/cbic.70436</a>. 660922026-06-30T12:44:05Z2026-06-30T13:01:42Z