On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays

K. Brassat, S. Ramakrishnan, J. Bürger, M. Hanke, M. Doostdar, J. Lindner, G. Grundmeier, A. Keller, Langmuir (2018).

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Abstract
DNA origami nanostructures are versatile substrates for the controlled arrangement of molecular capture sites with nanometer precision and thus have many promising applications in singlemolecule bioanalysis. Here, we investigate the adsorption of DNA origami nanostructures in nanohole arrays which represent an important class of biosensors and may benefit from the incorporation of DNA origami-based molecular probes. Nanoholes with well-defined diameter that enable the adsorption of single DNA origami triangles are fabricated in Au films on Siwafers by nanosphere lithography. The efficiency of directed DNA origami adsorption on the exposed SiO2 areas at the bottoms of the nanoholes is evaluated in dependence of various parameters, i.e., Mg2+ and DNA origami concentrations, buffer strength, adsorption time, and nanohole diameter. We observe that the buffer strength has a surprisingly strong effect on DNA origami adsorption in the nanoholes and that multiple DNA origami triangles with 120 nm edge length can adsorb in nanoholes as small as 120 nm in diameter. We attribute the latter observation to the low lateral mobility of once adsorbed DNA origami on the SiO2 surface, in combination with parasitic adsorption to the Au film. While parasitic adsorption can be suppressed by modifying the Au film with a hydrophobic self-assembled monolayer, the limited surface mobility of the adsorbed DNA origami still leads to poor localization accuracy in the nanoholes and results in many DNA origami crossing the boundary to the Au film even under optimized conditions. We discuss possible ways to minimize this effect by varying the composition of the adsorption buffer, employing different fabrication conditions, or using other substrate materials for nanohole array fabrication.
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Langmuir
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Brassat K, Ramakrishnan S, Bürger J, et al. On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays. Langmuir. 2018. doi:10.1021/acs.langmuir.8b00793
Brassat, K., Ramakrishnan, S., Bürger, J., Hanke, M., Doostdar, M., Lindner, J., … Keller, A. (2018). On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays. Langmuir. https://doi.org/10.1021/acs.langmuir.8b00793
@article{Brassat_Ramakrishnan_Bürger_Hanke_Doostdar_Lindner_Grundmeier_Keller_2018, title={On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays}, DOI={10.1021/acs.langmuir.8b00793}, journal={Langmuir}, publisher={American Chemical Society (ACS)}, author={Brassat, Katharina and Ramakrishnan, Saminathan and Bürger, Julius and Hanke, Marcel and Doostdar, Mahnaz and Lindner, Jörg and Grundmeier, Guido and Keller, Adrian}, year={2018} }
Brassat, Katharina, Saminathan Ramakrishnan, Julius Bürger, Marcel Hanke, Mahnaz Doostdar, Jörg Lindner, Guido Grundmeier, and Adrian Keller. “On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays.” Langmuir, 2018. https://doi.org/10.1021/acs.langmuir.8b00793.
K. Brassat et al., “On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays,” Langmuir, 2018.
Brassat, Katharina, et al. “On the Adsorption of DNA Origami Nanostructures in Nanohole Arrays.” Langmuir, American Chemical Society (ACS), 2018, doi:10.1021/acs.langmuir.8b00793.
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